05-173 | Anti-Calmodulin Antibody

05-173
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Po, R, VrtWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-173
      Replaces04-1158
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Calmodulin Antibody
      Alternate Names
      • calmodulin 1
      • calmodulin 1 (phosphorylase kinase, delta)
      • phosphorylase kinase, delta subunit
      Background InformationCalmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.
      CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.
      References
      Product Information
      FormatPurified
      Control
      • Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. Use 20 μg per lane for minigels.
      PresentationPurified mouse IgG1 in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationDetect Calmodulin using this Anti-Calmodulin Antibody validated for use in WB & IP.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation:
      4 μg of a previous lot immunoprecipitated calmodulin from A431 RIPA cell lysate. Radioimmunoassay:
      Use 10 μg/mL to detect calmodulin in the range of 200-300 ng.
      Biological Information
      ImmunogenSynthetic peptide corresponding to the 21 carboxy-terminal amino acids (128-148) of bovine calmodulin, conjugated with tyroglobulin.
      Epitopea.a. 128-148
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificitySpecific for both Ca2+-bound and Ca2+-free calmodulin, Mr 17 kDa. Does not recognize bovine S100-alpha, S100-beta, troponin C or parvalbumin.
      IsotypeIgG1
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      • Vertebrates
      Species Reactivity NoteAlso reacts to wheat germ. Wide cross-reactivity expected.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCalmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.[supplied by OMIM]
      Gene Symbol
      • CALM1
      • CALML2
      • CALML2
      • CALM3
      • CALM
      • CaM
      • CALM2
      • CAM2
      • CAMC
      • CAM3
      • DD132
      • CAMIII
      • PHKD
      • CAMI
      • CAMB
      • CAM
      • CAM1
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: Calmodulin mediates the control of a large number of enzymes and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CEP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis.

      SUBUNIT STRUCTURE: Interacts with MYO1C By similarity. Interacts with CEP97, CEP110, TTN/titin and SRY.

      SUBCELLULAR LOCATION: Spindle. Note= Distributed throughout the cell during interphase, but during mitosis becomes dramatically localized to the spindle poles and the spindle microtubules. Ref.20

      PTM Ubiquitination results in a strongly decreased activity By similarity.

      Phosphorylation results in a decreased activity By similarity.

      MISCELLANEOUS This protein has four functional calcium-binding sites.

      SEQUENCE SIMILARITIES Belongs to the calmodulin family.

      Contains 4 EF-hand domains.

      Sequence caution The sequence CAA36839.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated on human A431 carcinoma cells, RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.

      Western Blot Analysis: 0.1-1 μg/mL of this antibody detected calmodulin in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      05-173_2829830 2829830
      Anti-Calmodulin 2464555
      Anti-Calmodulin - 2123697 2123697
      Anti-Calmodulin - 15341 15341
      Anti-Calmodulin - 18130 18130
      Anti-Calmodulin - 18823 18823
      Anti-Calmodulin - 1969113 1969113
      Anti-Calmodulin - 2014188 2014188
      Anti-Calmodulin - 2073102 2073102
      Anti-Calmodulin - 2211097 2211097

      References

      Reference overviewApplicationPub Med ID
      Serotonin 5-HT3 receptor-mediated vomiting occurs via the activation of Ca2+/CaMKII-dependent ERK1/2 signaling in the least shrew (Cryptotis parva).
      Zhong, W; Hutchinson, TE; Chebolu, S; Darmani, NA
      PloS one  9  e104718  2014

      Show Abstract
      Western Blotting25121483 25121483
      Iqcg is essential for sperm flagellum formation in mice.
      Li, RK; Tan, JL; Chen, LT; Feng, JS; Liang, WX; Guo, XJ; Liu, P; Chen, Z; Sha, JH; Wang, YF; Chen, SJ
      PloS one  9  e98053  2014

      Show Abstract
      Western Blotting24849454 24849454
      Drosophila pericentrin requires interaction with calmodulin for its function at centrosomes and neuronal basal bodies but not at sperm basal bodies.
      Galletta, BJ; Guillen, RX; Fagerstrom, CJ; Brownlee, CW; Lerit, DA; Megraw, TL; Rogers, GC; Rusan, NM
      Molecular biology of the cell  25  2682-94  2014

      Show Abstract
      Immunofluorescence25031429 25031429
      A new method for quantitative immunoblotting of endogenous α-synuclein.
      Newman, AJ; Selkoe, D; Dettmer, U
      PloS one  8  e81314  2013

      Show Abstract
      24278419 24278419
      Endothelial nitric-oxide synthase activation generates an inducible nitric-oxide synthase-like output of nitric oxide in inflamed endothelium.
      Lowry, JL; Brovkovych, V; Zhang, Y; Skidgel, RA
      The Journal of biological chemistry  288  4174-93  2013

      Show Abstract
      23255592 23255592
      Metabolic regulation of CaMKII protein and caspases in Xenopus laevis egg extracts.
      McCoy, F; Darbandi, R; Chen, SI; Eckard, L; Dodd, K; Jones, K; Baucum, AJ; Gibbons, JA; Lin, SH; Colbran, RJ; Nutt, LK
      The Journal of biological chemistry  288  8838-48  2013

      Show Abstract
      23400775 23400775
      Identification of a calmodulin-binding domain in Sema4D that regulates its exodomain shedding in platelets.
      Mou, P; Zeng, Z; Li, Q; Liu, X; Xin, X; Wannemacher, KM; Ruan, C; Li, R; Brass, LF; Zhu, L
      Blood  121  4221-30  2013

      Show Abstract
      23564909 23564909
      In vivo cross-linking reveals principally oligomeric forms of α-synuclein and β-synuclein in neurons and non-neural cells.
      Dettmer, U; Newman, AJ; Luth, ES; Bartels, T; Selkoe, D
      The Journal of biological chemistry  288  6371-85  2013

      Show Abstract
      23319586 23319586
      Molecular architecture of the chick vestibular hair bundle.
      Shin, JB; Krey, JF; Hassan, A; Metlagel, Z; Tauscher, AN; Pagana, JM; Sherman, NE; Jeffery, ED; Spinelli, KJ; Zhao, H; Wilmarth, PA; Choi, D; David, LL; Auer, M; Barr-Gillespie, PG
      Nature neuroscience  16  365-74  2013

      Show Abstract
      23334578 23334578
      Significance of calcium binding, tyrosine phosphorylation, and lysine trimethylation for the essential function of calmodulin in vertebrate cells analyzed in a novel gene replacement system.
      Panina, S; Stephan, A; la Cour, JM; Jacobsen, K; Kallerup, LK; Bumbuleviciute, R; Knudsen, KV; Sánchez-González, P; Villalobo, A; Olesen, UH; Berchtold, MW
      The Journal of biological chemistry  287  18173-81  2012

      Show Abstract
      Western Blotting22493455 22493455

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies