06-661 | Anti-Calreticulin Antibody

06-661
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, Po, R, XnICC, IHC, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-661
      ReplacesMABT145
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Calreticulin Antibody
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.0, 0.15M NaCl and 0.05% sodium azide.
      Applications
      ApplicationAnti-Calreticulin Antibody is an antibody against Calreticulin for use in IC, IH & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Western Blotting
      Biological Information
      Immunogenpeptide (QAKDEL) corresponding to the C-terminus (amino acids 412-417) of human calreticulin
      HostRabbit
      Specificitycalreticulin; does not cross react with other endoplasmic reticulum proteins
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Pig
      • Rat
      • Xenopus
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCalreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.
      Gene Symbol
      • CALR
      • calreticulin
      • cC1qR
      • CRTC
      • HACBP
      • CRP55
      • grp60
      • FLJ26680
      • Calregulin
      • ERp60
      • RO
      • SSA
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P27797 # Molecular calcium binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
      SIZE: 417 amino acids; 48142 Da
      SUBUNIT: Monomer. Component of an EIF2 complex at least composed of CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 and with NR3C1 (By similarity).
      SUBCELLULAR LOCATION: Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Note=Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
      DOMAIN: SwissProt: P27797 Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity. & The interaction with glycans occurs through a binding site in the globular lectin domain. & The zinc binding sites are localized to the N-domain. & Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
      SIMILARITY: Belongs to the calreticulin family.
      Molecular Weight60kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from murine 3T3/NIH fibroblasts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Calreticulin (rabbit polyclonal IgG) - 2390425 2390425
      Anti-Calreticulin (rabbit polyclonal IgG) 2937382
      Anti-Calreticulin (rabbit polyclonal IgG) - 1972317 1972317
      Anti-Calreticulin (rabbit polyclonal IgG) - 2283838 2283838
      Anti-Calreticulin (rabbit polyclonal IgG) - 2377369 2377369
      Anti-Calreticulin - 16344 16344
      Anti-Calreticulin - 21385 21385
      Anti-Calreticulin - 28104 28104
      Anti-Calreticulin - 29700 29700
      Anti-Calreticulin - DAM1754434 DAM1754434

      References

      Reference overviewPub Med ID
      Regulation of calreticulin gene expression by calcium.
      Waser, M, et al.
      J. Cell Biol., 138: 547-57 (1997)  1997

      Show Abstract
      9245785 9245785
      Calreticulin.
      Krause, K H and Michalak, M
      Cell, 88: 439-43 (1997)  1997

      9038335 9038335
      Calreticulin is essential for integrin-mediated calcium signalling and cell adhesion.
      Coppolino, M G, et al.
      Nature, 386: 843-7 (1997)  1997

      Show Abstract
      9126744 9126744
      Calreticulin inhibits glucocorticoid- but not cAMP-sensitive expression of tyrosine aminotransferase gene in cultured McA-RH7777 hepatocytes.
      Burns, K, et al.
      Mol. Cell. Biochem., 171: 37-43 (1997)  1997

      Show Abstract
      9201693 9201693
      Endoplasmic reticulum form of calreticulin modulates glucocorticoid-sensitive gene expression.
      Michalak, M, et al.
      J. Biol. Chem., 271: 29436-45 (1996)  1996

      Show Abstract
      8910610 8910610
      Calreticulin, and not calsequestrin, is the major calcium binding protein of smooth muscle sarcoplasmic reticulum and liver endoplasmic reticulum.
      Milner, R E, et al.
      J. Biol. Chem., 266: 7155-65 (1991)  1991

      Show Abstract
      2016321 2016321

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies