05-1431 | Anti-Casein Kinase 2α Antibody, 1AD9

05-1431
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RICC, WB, IH(P), IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1431
      Replaces04-1129
      DescriptionAnti-Casein Kinase 2α Antibody, 1AD9
      Alternate Names
      • CK2 catalytic subunit alpha
      • CKII alpha
      • Casein kinase II subunit alpha
      • Casein kinase 2, alpha 1 polypeptide
      • Casein kinase II alpha 1 subunit
      • Casein kinase II-alpha subunit
      • Protein kinase CK2
      Background InformationUnlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate, pH 7.6 with 0.25 M NaCl and 0.1% sodium azide.
      Applications
      ApplicationAnti-Casein Kinase 2α Antibody, 1AD9 detects level of Casein Kinase 2α & has been published & validated for use in IC, WB, IH(P) & IP.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunoprecipitation: Recommended

      Immunocytochemistry: A previous lot of this antibody was used at a dilution of 5 μg/mL (acetone or methanol fix only).
      Optimal working dilutions must be determined by end user.

      Immunohistochemistry(paraffin): Representative testing from a previous lot.
      Optimal Staining of Casein Kinase 2 Monoclonal Antibody: S-KB-3 Cells
      Biological Information
      ImmunogenPurified recombinant α-subunit of casein kinase 2.
      EpitopeRegion common to both alpha and alpha' subunits
      Clone1AD9
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThe antibody binds specifically to the catalytic α-subunit of casein kinase2 (CK-2α). The antibody immunoprecipitates a protein with a molecular weight of 44 kD, CK-2α subunit. A 26 kD protein band, the β-subunit of the heterotetrameric holoenzyme α2β2, is coprecipitated with the CK-2α subunit. In western blots with extracts of kidney tissue, the antibody detects various protein bands between 40 and 44 kDa. Using 1 μg/mL of antibody, the lower detection limit for the holoenzyme is 500 ng in Western blot and 100 ng in dot blot; the detection limits for the α-subunit are 300 ng and 60 ng, respectively.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryCasein kinase II is a serine/threonine protein kinase that phosphorylates acidic proteins such as casein. The kinase exists as a tetramer and is composed of an alpha, an alpha-prime, and two beta subunits. The alpha subunits contain the catalytic activity while the beta subunits undergo autophosphorylation. The protein encoded by this gene represents the alpha subunit. While this gene is found on chromosome 20, a related transcribed pseudogene is found on chromosome 11. Three transcript variants encoding two different proteins have been found for this gene.
      Gene Symbol
      • CSNK2A1
      • CK2A1
      • CKII
      • CK II 3 EC 2.7.11.1
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P68400 # Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation.
      SIZE: 391 amino acids; 45144 Da
      SUBUNIT: Tetramer composed of an alpha chain, an alpha' and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1.
      SIMILARITY: SwissProt: P68400 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. & Contains 1 protein kinase domain
      Molecular Weight44 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected Neurofilament on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Casein Kinase 2#945;, clone 1AD9 - 2137043 2137043
      Anti-Casein Kinase 2, clone 1AD9 - 2035080 2035080
      Anti-Casein Kinase 2, clone 1AD9 - NG1577356 NG1577356
      Anti-Casein Kinase 2, clone 1AD9 - NG1661010 NG1661010
      Anti-Casein Kinase 2, clone 1AD9 - NG1734966 NG1734966
      Anti-Casein Kinase 2, clone 1AD9 - NG1843413 NG1843413
      Anti-Casein Kinase 2, clone 1AD9 - NG1895546 NG1895546
      Anti-Casein Kinase 2α, clone 1AD9 - 2420444 2420444
      Anti-Casein Kinase 2α, clone 1AD9 - 2436370 2436370
      Anti-Casein Kinase 2α, clone 1AD9 -2679359 2679359

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies