06-503 | Anti-Caspase 1 Antibody

06-503
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-503
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Caspase 1 Antibody
      Alternate Names
      • CASP1 nirs variant 1
      • IL-1 beta-converting enzyme
      • Interleukin-1 beta convertase
      • Interleukin-1 beta-converting enzyme
      • caspase 1
      • apoptosis-related cysteine peptidase (interleukin 1, beta convertase)
      • apoptosis-related cysteine protease (interleukin 1
      • beta, convertase)
      • interleukin 1-B converting enzyme
      • interleukin 1-beta convertase
      Background InformationCaspase-1, also known as IL-1 beta-converting enzyme (ICE), is an ezyme that cleaves the inflammatory cytokines IL-1 beta and IL-18, releasing the mature, active peptides. Caspase-1 is important for defense against pathogens and it also is involved in the induction of apoptosis. Thymocytes from caspase-1 deficient mice are resistant to Fas-mediated apoptosis, and caspase-1 is similar to the cell death gene CED-3 of C. elegans. Caspase-1 is a heterotetramer that consists of two anti-parallel heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The enzyme has a preferred cleavage site of Tyr-Val-Ala-Asp (YVAD). Caspase-1 is highly expressed in spleen and lung, and is absent from brain.
      References
      Product Information
      FormatPurified
      Control
      • Human HL-60
      PresentationPurified rabbit IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationAnti-Caspase 1 Antibody is an antibody against Caspase 1 for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      5 μg of a previous lot of this antibody immunoprecipitated p45 proenzyme form of Caspase 1 from 500 μg of human HL-60 cell lysates.

      Immunocytochemistry:
      10 μg/mL of a previous lot of this antibody gave positive immunostaining of HL60 cells.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acid residues 129-149 of human Caspase 1 (PEHKTSDSTFLVFMSHGIREG).
      Epitopea.a. 129-149
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes the p45 proenzyme, Mr 45 kDa, and the p20 subunit, Mr 20 kDa, of reduced human Caspase 1 isoforms.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat. Reactivity with other species has not been confirmed.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms.
      Gene Symbol
      • CASP1
      • IL1BCE
      • P45
      • IL-1BC
      • IL1BC
      • p45
      • IL1B-convertase
      • CASP-1
      • ICE
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P29466 # Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis.
      SIZE: 404 amino acids; 45159 Da
      SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunit. The p20 subunit can also form a heterodimer with the epsilon isoform which then has an inhibitory effect. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and NALP2 and whose function would be the activation of proinflammatory caspases.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
      PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
      SIMILARITY: SwissProt: P29466 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain. ... hide » see more »
      Molecular WeightProenzyme (45 kDa) and p20 subunit (20 kDa)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on cell lysate from human HL-60.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot of antibody detected the p45 proenzyme form of Caspase 1 in 20 μg of cell lysate from human HL-60. The immuno-reactivity can be inhibited by the immunizing peptide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Caspase 1 - 2142228 2142228
      Anti-Caspase 1 - 15672 15672
      Anti-Caspase 1 - 19084 19084
      Anti-Caspase 1 - 1957275 1957275
      Anti-Caspase 1 - 2032294 2032294
      Anti-Caspase 1 - 2073778 2073778
      Anti-Caspase 1 - 20910 20910
      Anti-Caspase 1 - 2116622 2116622
      Anti-Caspase 1 - 2166771 2166771
      Anti-Caspase 1 - 26416 26416

      References

      Reference overviewApplicationPub Med ID
      Human fetal membranes generate distinct cytokine profiles in response to bacterial Toll-like receptor and nod-like receptor agonists.
      Hoang, M; Potter, JA; Gysler, SM; Han, CS; Guller, S; Norwitz, ER; Abrahams, VM
      Biology of reproduction  90  39  2014

      Show Abstract
      24429216 24429216
      Cyclic-di-GMP and cyclic-di-AMP activate the NLRP3 inflammasome.
      Abdul-Sater, AA; Tattoli, I; Jin, L; Grajkowski, A; Levi, A; Koller, BH; Allen, IC; Beaucage, SL; Fitzgerald, KA; Ting, JP; Cambier, JC; Girardin, SE; Schindler, C
      EMBO reports  14  900-6  2013

      Show Abstract
      24008845 24008845
      Caspase-1 promotes Epstein-Barr virus replication by targeting the large tegument protein deneddylase to the nucleus of productively infected cells.
      Gastaldello, S; Chen, X; Callegari, S; Masucci, MG
      PLoS pathogens  9  e1003664  2013

      Show Abstract
      24130483 24130483
      Nod1, but not the ASC inflammasome, contributes to induction of IL-1β secretion in human trophoblasts after sensing of Chlamydia trachomatis.
      Kavathas, PB; Boeras, CM; Mulla, MJ; Abrahams, VM
      Mucosal immunology  6  235-43  2013

      Show Abstract
      22763410 22763410
      Caspase-1-mediated regulation of fibrogenesis in diet-induced steatohepatitis.
      Dixon, LJ; Berk, M; Thapaliya, S; Papouchado, BG; Feldstein, AE
      Laboratory investigation; a journal of technical methods and pathology  92  713-23  2012

      Show Abstract
      Immunohistochemistry22411067 22411067
      TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2.
      Delhase, M; Kim, SY; Lee, H; Naiki-Ito, A; Chen, Y; Ahn, ER; Murata, K; Kim, SJ; Lautsch, N; Kobayashi, KS; Shirai, T; Karin, M; Nakanishi, M
      Proceedings of the National Academy of Sciences of the United States of America  109  E177-86  2012

      Show Abstract
      22203995 22203995
      The calcium-sensing receptor regulates the NLRP3 inflammasome through Ca2+ and cAMP.
      Lee, GS; Subramanian, N; Kim, AI; Aksentijevich, I; Goldbach-Mansky, R; Sacks, DB; Germain, RN; Kastner, DL; Chae, JJ
      Nature  492  123-7  2012

      Show Abstract
      Western Blotting23143333 23143333
      Uric acid induces trophoblast IL-1β production via the inflammasome: implications for the pathogenesis of preeclampsia.
      Mulla, MJ; Myrtolli, K; Potter, J; Boeras, C; Kavathas, PB; Sfakianaki, AK; Tadesse, S; Norwitz, ER; Guller, S; Abrahams, VM
      American journal of reproductive immunology (New York, N.Y. : 1989)  65  542-8  2011

      Show Abstract
      21352397 21352397
      CONSTITUTIONAL METHYLATION OF THE BRCA1 PROMOTER IS SPECIFICALLY ASSOCIATED WITH BRCA1 MUTATION-ASSOCIATED PATHOLOGY IN EARLY-ONSET BREAST CANCER.
      Wong EM, Southey MC, Fox SB, Brown MA, Dowty JG, Jenkins MA, Giles GG, Hopper J, Dobrovic A
      Cancer Prev Res (Phila)  2010

      Show Abstract
      20978112 20978112
      Caspase-1 contributes to Chlamydia trachomatis-induced upper urogenital tract inflammatory pathologies without affecting the course of infection.
      Cheng, W; Shivshankar, P; Li, Z; Chen, L; Yeh, IT; Zhong, G
      Infection and immunity  76  515-22  2008

      Show Abstract
      18025098 18025098

      FAQ

      QuestionAnswer
      Why does caspase often exhibit different molecular weights?Full-length caspase 3 (pro-form) is 32kDa. Upon activation, caspase-3 is cleaved generating two smaller subunits of 17 kDa and 12 kDa. Other Caspases include Caspase 1 (proenzyme at 45kDa and subunit at 20kDa); Caspase 6 at 34kDa; Caspase 7 at 38kDa; Caspase 8 at 55kDa and Caspase 9 at 46-48kDa. It should be noted however that it can be difficult to detect the active fragments of many caspases because of their small size and biologically short life. The pro-forms are much more stable and readily detectable.

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      Life Science Research > Antibodies and Assays > Primary Antibodies