06-923 | Anti-Cdk1/Cdc2 (PSTAIR) Antibody

06-923
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Am, Ch, H, M, R, Sh, Dr, Mk, CaICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-923
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cdk1/Cdc2 (PSTAIR) Antibody
      Background InformationEntry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. Activation of cdc2 is controlled at several steps including cyclin binding and phosphorylation of Thr161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14. Phosphorylation at Tyr15 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase. Expression of cdc2 plays a critical role in cell transition through the G2/M phase. The activity of cdc2 in dividing cells increases at the onset of M phase coincident with disassembly of the cell nucleus, generation of mitotic spindles and chromosome condensation. Several proteins including Histone H1 and pp60src are substrates for cdc2.
      References
      Product Information
      FormatPurified
      Control
      • A431 cell lysate
      PresentationPurified rabbit serum in Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Cdk1/Cdc2 (PSTAIR) Antibody is a high quality Rabbit Polyclonal Antibody for the detection of Cdk1/Cdc2 (PSTAIR) & has been validated in ICC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesFluorescent Immunocytochemistry Analysis:
      A previous lot of this antibody was used in the confocal Immunofluorescent microscopy of A431 cells. Cells were grown, washed, fixed with formaldahyde, and permeabilized with NP40. The cells were triple stained with with Anti-Cdk1/Cdc2 (Red, Cy3), Phalloidin (Actin, Green, AlexaFluor®488), and DAPI (Blue, Nucleus). Positive nuclear staining.

      Immunoprecipitation: 4 µg of a previous lot immunoprecipitated cdc2 from 500 µg of A431 RIPA lysate.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues 42-57 of human cdc2 kinase. The immunogen is identical in a wide range of species.
      EpitopeN-terminus
      HostRabbit
      SpecificityCdk1/cdc2 kinase
      IsotypeIgG
      Species Reactivity
      • Amphibian
      • Chicken
      • Human
      • Mouse
      • Rat
      • Sheep
      • Drosophila
      • Monkey
      • Canine
      Species Reactivity NoteHuman, mouse and rat.
      Predicted to react in drosophila, monkey, canine, sheep, chicken, frog and starfish. Wide species cross-reactivity is expected.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control.
      Gene Symbol
      • CDC2
      • CDK1
      • MGC111195
      • DKFZp686L20222
      • CDC28A
      Purification MethodAntigen Affinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06493 # Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.
      SIZE: 297 amino acids; 34095 Da
      SUBUNIT: Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with DLG7. Isoform 2 is unable to complex with cyclin B1 and also fails to bind to the CDK inhibitor p21. Interacts with catalytically active CCNB1 and RALBP1 during mitosis to form an endocytotic complex during interphase.
      SUBCELLULAR LOCATION: Nucleus (By similarity).
      SIMILARITY: SwissProt: P06493 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
      Molecular Weight~34 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on human A431, mouse 3T3 and/or rat L6 RIPA cell lysates.

      Western Blot Analysis: 0.5-2 µg/mL of this antibody detected cdc2 from human A431, mouse 3T3 or rat L6 RIPA cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cdk1/Cdc2 (PSTAIR) - 1955413 1955413
      Anti-Cdk1/Cdc2 (PSTAIR) - 2043525 2043525
      Anti-Cdk1/Cdc2 (PSTAIR) - 2087931 2087931
      Anti-Cdk1/Cdc2 (PSTAIR) - 2283847 2283847
      Anti-Cdk1/Cdc2 (PSTAIR) - DAM1718060 DAM1718060
      Anti-Cdk1/Cdc2 (PSTAIR) - DAM1764427 DAM1764427
      Anti-Cdk1/Cdc2 (PSTAIR) - JBC1922551 JBC1922551
      Anti-Cdk1/Cdc2 (PSTAIR) - JBC1946380 JBC1946380
      Anti-Cdk1/Cdc2 (PSTAIR) -2601673 2601673
      Anti-Cdk1/Cdc2 (PSTAIR) -2685470 2685470

      References

      Reference overviewApplicationSpeciesPub Med ID
      Cdk1 inhibition induces mutually inhibitory apoptosis and reactivation of Kaposi's sarcoma-associated herpesvirus.
      Li, X; Chen, S; Sun, R
      Journal of virology  86  6668-76  2012

      Show Abstract
      22496227 22496227
      Iron-independent phosphorylation of iron regulatory protein 2 regulates ferritin during the cell cycle.
      Wallander, ML; Zumbrennen, KB; Rodansky, ES; Romney, SJ; Leibold, EA
      The Journal of biological chemistry  283  23589-98  2008

      Show Abstract
      18574241 18574241
      Plk1-dependent phosphorylation regulates functions of DNA topoisomerase IIalpha in cell cycle progression.
      Li, H; Wang, Y; Liu, X
      The Journal of biological chemistry  283  6209-21  2008

      Show Abstract
      18171681 18171681
      Phosphorylation of Plk1 at Ser326 regulates its functions during mitotic progression.
      Tang, J; Yang, X; Liu, X
      Oncogene  27  6635-45  2008

      Show Abstract Full Text Article
      18695677 18695677
      An essential role for Cdk1 in S phase control is revealed via chemical genetics in vertebrate cells.
      Hochegger, H; Dejsuphong, D; Sonoda, E; Saberi, A; Rajendra, E; Kirk, J; Hunt, T; Takeda, S
      The Journal of cell biology  178  257-68  2007

      Show Abstract
      Fluorescence Activated Cell Sorting (FACS)17635936 17635936
      Up-regulation of 14-3-3zeta in lung cancer and its implication as prognostic and therapeutic target.
      Fan, T; Li, R; Todd, NW; Qiu, Q; Fang, HB; Wang, H; Shen, J; Zhao, RY; Caraway, NP; Katz, RL; Stass, SA; Jiang, F
      Cancer research  67  7901-6  2007

      Show Abstract
      Western BlottingHuman17699796 17699796
      Quantification of cyclin B1 and p34(cdc2) in bovine cumulus-oocyte complexes and expression mapping of genes involved in the cell cycle by complementary DNA macroarrays.
      Claude Robert, Isabelle Hue, Serge McGraw, Dominic Gagné, Marc-André Sirard
      Biology of reproduction  67  1456-64  2002

      Show Abstract
      12390876 12390876
      Platelet-activating factor- and thrombin-induced stimulation of p34cdc2-cyclin histone H1 kinase activity in platelets.
      Samiei, M, et al.
      J. Biol. Chem., 266: 14889-92 (1991)  1991

      Show Abstract
      1869529 1869529
      Complementation used to clone a human homologue of the fission yeast cell cycle control gene cdc2
      Lee, M G and Nurse, P
      Nature, 327:31-5 ()  1987

      3553962 3553962

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies