06-1284 | Anti-Cdt1 (NT) Antibody

06-1284
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbSerumPolyclonal Antibody
      Description
      Catalogue Number06-1284
      DescriptionAnti-Cdt1 (NT) Antibody
      Alternate Names
      • DNA replication factor
      • Double parked homolog
      Background InformationChromatin licensing and DNA replication factor 1, also known as CDT1, is a key licensing factor which, along with the protein Cdc6, functions to license DNA by forming the pre-replicative complex (pre-RC). Its activity during the cell cycle is tightly regulated by its association with the protein geminin, which both inhibits Cdt1 activity during S phase in order to prevent re-replication of DNA and prevents it from ubiquitination and subsequent proteolysis.
      References
      Product Information
      FormatSerum
      Control
      • Hela/Nocodozole lysate
      PresentationRabbit polyclonal serum containing 0.05% sodium azide.
      Applications
      ApplicationAnti-Cdt1 (N-terminus) Antibody is a Rabbit Polyclonal Antibody for detection of Cdt1 (N-terminus) also known as DNA replication factor, Double parked homolog & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to the N-terminal region of CDT1.
      EpitopeN-terminus
      HostRabbit
      SpecificityThis antibody recognizes CDT1 at and around the N-terminus.
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human.
      Sequence homology: (71% mouse) and (70% rat).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DUP
      • RIS2
      Purification MethodSerum
      UniProt Number
      UniProt SummaryFUNCTION: Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
      SUBUNIT STRUCTURE: Interaction with GMNN inhibits both binding of the MCM complex to origins of replication and DNA-binding activity.
      SUBCELLUAR LOCATION: Nucleus.
      DEVELOPMENTAL STAGE: Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
      PTM: Ubiquitinated, leading to its subsequent degradation. The interaction with GMNN protects it against ubiquitination.
      Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
      SEQUENCE SIMILARITIES: Belongs to the Cdt1 family.
      SEQUENCE CAUTION: The sequence AF070552 differs from that shown. Reason: Frameshift at positions 278 and 312.
      Molecular Weight~65 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Hela/Nocodozole lysate.
      Western Blot Analysis: 1:1000 dilution of this antibody detected CDT1 in 10 µg of HeLa/Nocodozole lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies