Key Spec Table
|Species Reactivity||Key Applications||Host||Format||Antibody Type|
|Gp, H, Rb||ELISA, IHC, WB||M||Purified||Monoclonal Antibody|
|Description||Anti-Complement C3a Antibody, clone H13|
|Presentation||Protein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water.|
|Application||This Anti-Complement C3a Antibody, clone H13 is validated for use in ELISA, IH, WB for the detection of Complement C3a.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Maintain at 2°-8°C for up to 12 months from date of receipt.|
|Material Size||50 µg|
|Reference overview||Pub Med ID|
|Quantitation of the anaphylatoxin C3a in the presence of C3 by a novel sandwich ELISA using monoclonal antibody to a C3a neoepitope.|
Zilow, G, et al.
J. Immunol. Methods, 121: 261-8 (1989) 1989
C3a levels in plasma are usually measured by a competitive inhibition radioimmunoassay (RIA) using 125I-labelled C3a-desArg and antibodies to C3a capable of detecting C3a determinants which are also present on the native C3. Therefore, prior to the assay native, non-cleaved C3 has to be removed completely from the C3a-containing sample by precipitation. We developed a new rapid two-site sandwich ELISA system for the quantitation of C3a-desArg in plasma. This immunoassay uses a monoclonal antibody (mAb H466) reacting with C3a-desArg but not with C3. The reactivity of mAb H466 with a neoantigenic determinant of C3a-desArg permitted the direct quantitation of C3a-desArg without removal of C3 from the sample. The mAb H466 was used as a capture antibody and bound C3a-desArg was detected with a second peroxidase-labelled anti-C3a mAb. The lower limit of detection of C3a-desArg in this ELISA was 1 ng/ml. The C3a-desArg levels measured in the plasma samples of various patients were found to differ over a wide range. A good correlation was observed between the results obtained in the RIA and those obtained in the ELISA (r = 0.95). High levels of C3a-desArg were detected in plasma from patients with multiple trauma and patients undergoing haemodialysis. The C3a-desArg assay described should facilitate the routine quantitation of C3a in samples of plasma.
|Anti-Complement C3a, clone H13 - Data Sheet|