CBL191 | Anti-Complement C3a Antibody, clone H13

CBL191
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Gp, H, RbELISA, IHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL191
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Complement C3a Antibody, clone H13
      References
      Product Information
      FormatPurified
      PresentationProtein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water.
      Applications
      ApplicationThis Anti-Complement C3a Antibody, clone H13 is validated for use in ELISA, IH, WB for the detection of Complement C3a.
      Key Applications
      • ELISA
      • Immunohistochemistry
      • Western Blotting
      Biological Information
      ImmunogenHuman complement component C3
      CloneH13
      HostMouse
      SpecificityThe antibody recognizes an epitope on the alpha-chain of Complement C3 (Western blot). The antibody inhibits the anaphylactic function of C3a: the capacity of C3a to induce serotonin release from thrombocytes in vitro is inhibited; similarly, treatment of C3a with the antibody inhibits the C3a-induced skin reaction in vivo. The antibody is, in combination with antibodies to the C3b fragment, an excellent control for distinguishing ubiquitous or nonspecifically bound native C3 from the biologically active C3 fragment. Nonactivated C3 still bears the C3a determinant, whereas activated C3 (C3b, C3d) is C3a-negative. It is therefore useful for immunopathological assays (demonstration of C3 deposition in biopsies) and for C3a assays.
      IsotypeIgG2b
      Species Reactivity
      • Guinea Pig
      • Human
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryComplement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. People with C3 deficiency are susceptable to bacteria infection.
      Gene Symbol
      • C3
      • ASP
      • ARMD9
      • CPAMD1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01024 # Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
      SIZE: 1663 amino acids; 187148 Da
      SUBUNIT: C3 precursor is first processed by the removal of 4 Arg residues, forming two chains, beta and alpha, linked by a disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylatoxin and generating C3b (beta chain + alpha' chain). During pregnancy, C3dg exists as a complex (probably a 2:2:2 heterohexamer) with AGT and the proform of PRG2. Interacts with CR2 and VSIG4.
      SUBCELLULAR LOCATION: Secreted.
      PTM: C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
      DISEASE: SwissProt: P01024 # Defects in C3 are the cause of C3 deficiency [MIM:120700]. It can result in susceptibility to pyogenic infection. & Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
      SIMILARITY: SwissProt: P01024 ## Contains 1 anaphylatoxin-like domain. & Contains 1 NTR domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2°-8°C for up to 12 months from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN COMPLEMENT C3A 2477808
      MOUSE ANTI-HUMAN COMPLEMENT C3A 2487588
      MOUSE ANTI-HUMAN COMPLEMENT C3A 2475824
      MOUSE ANTI-HUMAN COMPLEMENT C3A - 2506089 2506089
      MOUSE ANTI-HUMAN COMPLEMENT C3A - 2950639 2950639
      MOUSE ANTI-HUMAN COMPLEMENT C3A - 2993041 2993041
      MOUSE ANTI-HUMAN COMPLEMENT C3A -2571600 2571600
      MOUSE ANTI-HUMAN COMPLEMENT C3A -2628347 2628347
      MOUSE ANTI-HUMAN COMPLEMENT C3A -2654653 2654653
      MOUSE ANTI-HUMAN COMPLEMENT C3A MONOCLONAL ANTIBODY 2897012

      References

      Reference overviewPub Med ID
      Quantitation of the anaphylatoxin C3a in the presence of C3 by a novel sandwich ELISA using monoclonal antibody to a C3a neoepitope.
      Zilow, G, et al.
      J. Immunol. Methods, 121: 261-8 (1989)  1989

      Show Abstract
      2503562 2503562

      Data Sheet

      Title
      Anti-Complement C3a, clone H13 - Data Sheet

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies