MABF983 | Anti-Complement C3b/iC3b/C3dg Antibody, clone 1H8

100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, PmFCMPurifiedMonoclonal Antibody
      Catalogue NumberMABF983
      DescriptionAnti-Complement C3b/iC3b/C3dg Antibody, clone 1H8
      Alternate Names
      • Complement C3
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1
      • Complement C3 beta chain
      • C3-beta-c
      • C3bc
      • Complement C3 alpha chain
      • C3a anaphylatoxin
      • Acylation stimulating protein
      • ASP
      • C3adesArg
      • Complement C3b alpha' chain
      • Complement C3c alpha' chain fragment 1
      • Complement C3dg fragment
      • Complement C3g fragment
      • Complement C3d fragment
      • Complement C3f fragment
      • Complement C3c alpha' chain fragment 2
      • Complement C3b/iC3b/C3dg
      Background InformationComplement C3 (UniProt P01024; also known as C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1) is encoded by the C3 (also known as CPAMD1) gene (Gene ID 718) in human. C3 is initially translated with an N-terminal 22-amino acid signal peptide sequence, which is then removed to produce the 1641-amino acid mature C3. It plays a central role in the activation of complement system. Its activation is required for the activation of both classical and alternative pathway of complement (CPC and APC, respectively). C3 is cleaved into C3a and C3b during CPC activation by the C3-convertase C4b2a composed of the activated C4 and C2. In APC, C3 is cleaved by the C3-convertase C3bBb composed C3b and the activated form of factor B (Bb). C3b serves as an opsonizing agent, and can be further cleaved by Factor I into C3c and C3d. iC3b is a proteolytically inactive C3b fragment that still opsonizes target microbes or cells, but cannot further amplify/activate the complement cascade through APC. iC3b can be further cleaved to C3dg, and finally to C3d. Unregulated activation of APC can result in paroxysmal nocturnal hemoglobinuria (PNH) that is characterized by chronic intravascular hemolysis. Clinical C5-neutralizing mAb treatment prevents the formation of cytolytic membrane attack complex (MAC) of complement, but does not block APC activation. Consequently, PNH patients are left with immune-mediated hemolytic anemia and their erythrocytes become opsonized with complement C3. Monoclonal antibodies (mAbs) against C3b/iC3b are useful for monitoring and studying C3b/iC3b deposit on PNH blood cells and mAbs with neutralizing activities are useful tools for studying C3-mediated CPC and APC.
      Product Information
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing PBS without preservatives.
      ApplicationThis mouse monoclonal Anti-Complement C3b/iC3b/C3dg Antibody, clone 1H8, Cat. No. MABF983 is validated for use in Flow Cytometry, for the detection of C3b.
      Key Applications
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: A presentative lot detected C3b/iC3b/C3dg deposit on paroxysmal nocturnal hemoglobinuria (PNH) patient-derived erythrocyte ghosts due to alternative pathway of complement (APC) activation-mediated hemolysis in acidified normal human serum (aNHS; pH 6.4) (Lindorfer, M.A., et al. (2010). Blood. 115(11):2283-2291).
      Biological Information
      ImmunogenSepharose 4B beads with surface C3b/C3bi deposits via APC in normal human serum corresponding to the iC3dg of human Complement C3b/iC3b/C3dg.
      ConcentrationPlease refer to lot specific datasheet.
      SpecificityThis clone binds C3b, iC3b, and C3dg, but does not block the activation of alternative pathway of complement (APC).
      Species Reactivity
      • Human
      • Primate
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • C3
      • CPAMD1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight187 kDa calculated
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceFlow Cytometry Analysis: This antibody (200 ug mAb/5 x 10e6 cells/mL) detected C3b/iC3b/C3dg deposit on human Burkett's lymphoma Raji B cells opsonized with anti-CD20 mAb Rituximab (RTX) in the presence of 50% normal human serum (NHS).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Complement C3b/iC3b/C3dg, clone 1H8 -Q2583310 Q2583310


      Reference overviewPub Med ID
      A novel approach to preventing the hemolysis of paroxysmal nocturnal hemoglobinuria: both complement-mediated cytolysis and C3 deposition are blocked by a monoclonal antibody specific for the alternative pathway of complement.
      Lindorfer, MA; Pawluczkowycz, AW; Peek, EM; Hickman, K; Taylor, RP; Parker, CJ
      Blood  115  2283-91  2010

      Show Abstract
      20068220 20068220

      Technical Info

      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.

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      Life Science Research > Antibodies and Assays > Primary Antibodies