05-373 | Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12)

05-373
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-373
      Replaces04-220
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cyclin B1 Antibody, clone GNS3 (8A5D12)
      Alternate Names
      • G2/mitotic-specific cyclin B1
      • cyclin B1
      Background InformationRegulation of cell cycle progression in eukaryotic cells depends on the expression of cyclin proteins. These proteins are the regulatory subunits of the cell cycle-dependent kinases that are responsible for the phosphorylation of several cellular targets. The B cyclins, when combined with p34cdc2, a catalytic subunit of the cell cycle dependent kinase, form an enzyme that is active at the transition to the G2 phase, attaining maximum level at M phase. At that phase, the complex associates with condensed chromosomes in prophase and metaphase and is degraded at the metaphase/anaphase transition.
      Anti-Cyclin B1 may be used for the detection of cyclin B1 expression and for measuring relative differences in cyclin B1 level as a function of cell cycle phase.
      References
      Product Information
      FormatPurified
      Control
      • A431 cells
        Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationProtein G purified mouse IgGs in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen at -20°C.
      Applications
      ApplicationAnti-Cyclin B1 Antibody, clone GNS3 (8A5D12) is a Mouse Monoclonal Antibody for detection of Cyclin B1 also known as G2/mitotic-specific cyclin B1 & has been validated in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      2 μg of a previous lot immunoprecipitated human cyclin B1 and cdc2 kinase from 500 μg of A431 RIPA lysate as determined by a subsequent immunoblot of the immunoprecipitate using anti-cdk1/cdc2 (PSTAIR), (Catalog # 06-923).
      Biological Information
      ImmunogenRecombinant human cyclin B1
      CloneGNS3 (8A5D12)
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody is specific for human cyclin B1, Mr 58 kDa. Does not cross-react with cyclin A or cyclin D.
      Isotypemixed IgGs
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteIn addition to human, weak species cross-reactivity was observed with mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.
      Gene Symbol
      • CCNB1
      • CCNB
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14635 # Essential for the control of the cell cycle at the G2/M (mitosis) transition.
      SIZE: 433 amino acids; 48337 Da
      SUBUNIT: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Centrosome.
      DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis.
      PTM: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
      SIMILARITY: SwissProt: P14635 ## Belongs to the cyclin family. Cyclin AB subfamily.
      Molecular Weight58 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot analysis on RIPA lysate from human A431 carcinoma cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected cyclin B1 in RIPA lysates from human A431 carcinoma cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cyclin B1 - 17772 17772
      Anti-Cyclin B1 - 1942575 1942575
      Anti-Cyclin B1 - 1984833 1984833
      Anti-Cyclin B1 - 2080303 2080303
      Anti-Cyclin B1 - 2111281 2111281
      Anti-Cyclin B1 - 21920 21920
      Anti-Cyclin B1 - 2199734 2199734
      Anti-Cyclin B1 - 2327462 2327462
      Anti-Cyclin B1 - 24012 24012
      Anti-Cyclin B1 - 26051 26051

      References

      Reference overviewApplicationPub Med ID
      Rac1-dependent recruitment of PAK2 to G2 phase centrosomes and their roles in the regulation of mitotic entry.
      May, M; Schelle, I; Brakebusch, C; Rottner, K; Genth, H
      Cell cycle (Georgetown, Tex.)  13  2211-21  2014

      Show Abstract
      24840740 24840740
      Parvovirus-induced depletion of cyclin B1 prevents mitotic entry of infected cells.
      Adeyemi, RO; Pintel, DJ
      PLoS pathogens  10  e1003891  2014

      Show Abstract
      24415942 24415942
      The deubiquitylase USP15 stabilizes newly synthesized REST and rescues its expression at mitotic exit.
      Faronato, M; Patel, V; Darling, S; Dearden, L; Clague, MJ; Urbé, S; Coulson, JM
      Cell cycle (Georgetown, Tex.)  12  1964-77  2013

      Show Abstract
      23708518 23708518
      Mitotic arrest and apoptosis in breast cancer cells induced by Origanum majorana extract: upregulation of TNF-α and downregulation of survivin and mutant p53.
      Al Dhaheri, Y; Eid, A; AbuQamar, S; Attoub, S; Khasawneh, M; Aiche, G; Hisaindee, S; Iratni, R
      PloS one  8  e56649  2013

      Show Abstract
      23451065 23451065
      The stress-activated protein kinases p38α/β and JNK1/2 cooperate with Chk1 to inhibit mitotic entry upon DNA replication arrest.
      Llopis, A; Salvador, N; Ercilla, A; Guaita-Esteruelas, S; Barrantes, Idel B; Gupta, J; Gaestel, M; Davis, RJ; Nebreda, AR; Agell, N
      Cell cycle (Georgetown, Tex.)  11  3627-37  2012

      Show Abstract
      22935704 22935704
      Sensitivity of cancer cells to Plk1 inhibitor GSK461364A is associated with loss of p53 function and chromosome instability.
      Degenhardt, Y; Greshock, J; Laquerre, S; Gilmartin, AG; Jing, J; Richter, M; Zhang, X; Bleam, M; Halsey, W; Hughes, A; Moy, C; Liu-Sullivan, N; Powers, S; Bachman, K; Jackson, J; Weber, B; Wooster, R
      Molecular cancer therapeutics  9  2079-89  2010

      Show Abstract
      20571075 20571075
      Clustering phenotype populations by genome-wide RNAi and multiparametric imaging.
      Florian Fuchs,Gregoire Pau,Dominique Kranz,Oleg Sklyar,Christoph Budjan,Sandra Steinbrink,Thomas Horn,Angelika Pedal,Wolfgang Huber,Michael Boutros
      Molecular systems biology  6  2010

      Show Abstract Full Text Article
      20531400 20531400
      Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression.
      Dulla, K; Daub, H; Hornberger, R; Nigg, EA; Körner, R
      Molecular & cellular proteomics : MCP  9  1167-81  2010

      Show Abstract
      20097925 20097925
      Phosphorylation-dependent binding of cyclin B1 to a Cdc6-like domain of human separase.
      Boos, D; Kuffer, C; Lenobel, R; Körner, R; Stemmann, O
      The Journal of biological chemistry  283  816-23  2008

      Show Abstract
      Immunoprecipitation17974570 17974570
      FLJ10540-elicited cell transformation is through the activation of PI3-kinase/AKT pathway.
      Chen, C-H, et al.
      Oncogene, 26: 4272-83 (2007)  2007

      17237822 17237822

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies