05-362 | Anti-Cyclin D1/2 Antibody, clone 5D4

05-362
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ht, M, RICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-362
      Replaces04-1151
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Cyclin D1/2 Antibody, clone 5D4
      Background InformationDuring each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by acting as regulatory subunits of the Cdks. Early in the G1 phase of the cell cycle, cyclin D1 induction is followed by cyclin E induction. This sequential progression is marked early on in G1 by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB. The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2. Cyclin D2 is also a G1 cyclin required for G1 phase progression and is a strong candidate for a proto oncogene. Cyclin D2 can phosphorylate pRB when associated with cdk 4 and / or cdk 6.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
      PresentationProtein A Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-Cyclin D1/2 Antibody, clone 5D4 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin D1/2 & has been validated in ICC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenRecombinant human cyclin D1
      Clone5D4
      HostMouse
      SpecificityRecognizes p36 cyclin D1 and p34 cyclin D2; no cross-reactivity with cyclin A, B or D3.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Hamster
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.
      Gene Symbol
      • CCND1
      • D11S287E
      • U21B31
      • PRAD1
      • BCL1
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P24385 # Essential for the control of the cell cycle at the G1/S (start) transition.
      SIZE: 295 amino acids; 33729 Da
      SUBUNIT: Interacts with the CDK4 and CDK6 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex.
      DISEASE: SwissProt: P24385 # A chromosomal aberration involving CCND1 may be a cause of B-lymphocytic malignancy, particularly mantle-cell lymphoma (MCL). Translocation t(11;14)(q13;q32) with immunoglobulin gene regions. Activation of CCND1 may be oncogenic by directly altering progression through the cell cycle. & A chromosomal aberration involving CCND1 may be a cause of parathyroid adenomas [MIM:168461]. Translocation t(11;11)(q13;p15) with the parathyroid hormone (PTH) enhancer. & A chromosomal aberration involving CCND1 may be a cause of multiple myeloma [MIM:254500]. Translocation t(11;14)(q13;q32) with the IgH locus.
      SIMILARITY: SwissProt: P24385 ## Belongs to the cyclin family. Cyclin D subfamily.
      Molecular Weight34/36 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysate from human A431 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Cyclin D1/2, clone 5D4 - 2138537 2138537
      Anti-Cyclin D1/2, clone 5D4 - 16554 16554
      Anti-Cyclin D1/2, clone 5D4 - 16893 16893
      Anti-Cyclin D1/2, clone 5D4 - 17366 17366
      Anti-Cyclin D1/2, clone 5D4 - 17366a 17366a
      Anti-Cyclin D1/2, clone 5D4 - 18385 18385
      Anti-Cyclin D1/2, clone 5D4 - 18385a 18385a
      Anti-Cyclin D1/2, clone 5D4 - 1952440 1952440
      Anti-Cyclin D1/2, clone 5D4 - 1976902 1976902
      Anti-Cyclin D1/2, clone 5D4 - 21115 21115

      References

      Reference overviewApplicationPub Med ID
      Failure of cell cleavage induces senescence in tetraploid primary cells.
      Panopoulos, A; Pacios-Bras, C; Choi, J; Yenjerla, M; Sussman, MA; Fotedar, R; Margolis, RL
      Molecular biology of the cell  25  3105-18  2014

      Show Abstract
      Western Blotting25143403 25143403
      Serine phosphorylation regulates disabled-1 early isoform turnover independently of Reelin.
      Gao, Z; Godbout, R
      Cellular signalling  23  555-65  2011

      Show Abstract
      Western Blotting21111810 21111810
      Akt2-mediated phosphorylation of Pitx2 controls Ccnd1 mRNA decay during muscle cell differentiation.
      R Gherzi,M Trabucchi,M Ponassi,I-E Gallouzi,M G Rosenfeld,P Briata
      Cell death and differentiation  17  2010

      Show Abstract
      20019746 20019746
      Cooperative signaling between Wnt1 and integrin-linked kinase induces accelerated breast tumor development.
      Oloumi, A; Maidan, M; Lock, FE; Tearle, H; McKinney, S; Muller, WJ; Aparicio, SA; Dedhar, S
      Breast cancer research : BCR  12  R38  2010

      Show Abstract
      Western Blotting20565980 20565980
      INK4a/Arf is required for suppression of EGFR/DeltaEGFR(2-7)-dependent ERK activation in mouse astrocytes and glioma.
      Lachat, Y; Diserens, AC; Nozaki, M; Kobayashi, H; Hamou, MF; Godard, S; De Tribolet, N; Hegi, ME
      Oncogene  23  6854-63  2004

      Show Abstract
      Immunohistochemistry15273738 15273738
      A non-thiazolidinedione partial peroxisome proliferator-activated receptor gamma ligand inhibits vascular smooth muscle cell growth.
      Dennis Bruemmer, Joel P Berger, Joey Liu, Ulrich Kintscher, Shu Wakino, Eckart Fleck, David E Moller, Ronald E Law, Dennis Bruemmer, Joel P Berger, Joey Liu, Ulrich Kintscher, Shu Wakino, Eckart Fleck, David E Moller, Ronald E Law
      European journal of pharmacology  466  225-34  2003

      Show Abstract
      12694805 12694805
      Tumor suppressor PTEN inhibits nuclear accumulation of beta-catenin and T cell/lymphoid enhancer factor 1-mediated transcriptional activation.
      Persad, S, et al.
      J. Cell Biol., 153: 1161-74 (2001)  2001

      Show Abstract
      Immunoblotting (Western)11402061 11402061
      Dual stimulation of Ras/mitogen-activated protein kinase and RhoA by cell adhesion to fibronectin supports growth factor-stimulated cell cycle progression
      Danen, E. H., et al
      J Cell Biol, 151:1413-22 (2000)  2000

      Immunoblotting (Western)11134071 11134071
      NA22598, a novel antitumor compound, reduces cyclin D1 levels, arrests cell cycle at G1 phase, and inhibits anchorage-independent growth of human tumor cells.
      M Kawada, A Kuwahara, T Nishikiori, S Mizuno, Y Uehara
      Experimental cell research  249  240-7  1999

      Show Abstract
      10366423 10366423
      Monoclonal antibody against PRAD1/cyclin D1 stains nuclei of tumor cells with translocation or amplification at BCL-1 locus.
      Banno, S, et al.
      Jpn. J. Cancer Res., 85: 918-26 (1994)  1994

      7961120 7961120

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      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies