CBL208 | Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8

CBL208
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL208
      ReplacesMABT150
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytokeratin 20 Antibody, clone IT-Ks20.8
      Background InformationIT-Ks 20.8 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative.
      References
      Product Information
      FormatPurified
      Control
      • Positive cell lines include HT-29, LoVo, DLD-1, SW 1116, CaCo-2 and RT-4.
      PresentationProtein A purified immunoglobulin lyophilized from PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide. Reconstitute with 1 mL distilled water.
      Applications
      ApplicationDetect Cytokeratin 20 using this Anti-Cytokeratin 20 Antibody, clone IT-Ks20.8 validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blotting
      Immunohistochemistry: 1:10 dilution of stock on frozen tissues, with one hour incubation at room temp or overnight at 2°-8°C. For paraffin sections, 1:100 dilution of antibody with protease pretreatment and/or heat induced epitope retrieval recommended.
      Cytological material

      Studies of cultured cell lines HT-29, LoVo, DLD-1, SW1116, CaCo-2 & RT-4.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenElectrophoretically purified cytokeratin 20 from human intestinal mucosa
      CloneIT-Ks20.8
      HostMouse
      SpecificityThe antibody is specific for the 46 kDa polypeptide (cytokeratin 20).
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
      Gene Symbol
      • KRT20
      • K20
      • CK20
      • Keratin-20
      • CK-20
      • KRT21
      • Cytokeratin-20
      • CD20
      • MGC35423
      Non-Reactive Species
      • Mouse
      • Rat
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35900 # Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
      SIZE: 424 amino acids; 48487 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. Associates with KRT8.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa,and in the taste buds of lingual mucosa.
      DEVELOPMENTAL STAGE: First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low- differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative.
      PTM: Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity). & Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
      SIMILARITY: SwissProt: P35900 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsOnce reconstituted, maintain at 2°-8°C for up to 12 months from date of receipt. For extended storage, aliquots can be stored at -20°C. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN CYTOKERATIN 20 MONOCLONAL ANTIBODY - 2120872 2120872
      MOUSE ANTI-HUMAN CYTOKERATIN 20 MONOCLONAL ANTIBODY - 2399448 2399448
      MOUSE ANTI-HUMAN CYTOKERATIN 20 MONOCLONAL ANTIBODY - 2266448 2266448

      References

      Reference overviewPub Med ID
      The distribution and origin of keratin 20-containing taste buds in rat and human.
      Zhang, C and Oakley, B
      Differentiation, 61: 121-7 (1996)  1996

      8983178 8983178
      Cytokeratin 20 as an objective marker of urothelial dysplasia.
      Harnden, P, et al.
      British journal of urology, 78: 870-5 (1996)  1996

      Show Abstract
      9014711 9014711
      Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins.
      Demirkesen, C, et al.
      J. Cutan. Pathol., 22: 518-35 (1995)  1995

      Show Abstract
      8835171 8835171
      Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis.
      Möbus, V J, et al.
      Br. J. Cancer, 69: 422-8 (1994)  1994

      Show Abstract
      7510115 7510115
      Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas.
      Moll, I and Moll, R
      Arch. Dermatol. Res., 283: 300-9 (1991)  1991

      Show Abstract
      1718227 1718227

      Data Sheet

      Title
      Anti-Cytokeratin 20, clone IT-Ks20.8 - Data Sheet

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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies