CBL194F-25ASSAYS | Anti-Cytokeratin 7, clone LP5K,FITCConj

NewCBL194F-25ASSAYS
25 assays  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHC, IH(P), FCMFITCMonoclonal Antibody
      Description
      Catalogue NumberCBL194F-25ASSAYS
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytokeratin 7, clone LP5K,FITCConj
      References
      Product Information
      FormatFITC
      PresentationThe antibody is ammonium sulphate precipitation followed by ion exchange chromatography. The conjugate is supplied in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin.
      Applications
      ApplicationDetect Cytokeratin 7 using this Anti-Cytokeratin 7 Antibody, clone LP5K, FITC conjugated validated for use in IC, IH, IH(P), FC.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      Application NotesImmunohistochemistry: Suitable for staining frozen sections and paraffin wax embedded tissue. Protease treatment of paraffin embedded sections is required prior to antibody application. Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).

      Immunocytochemistry: isolated EVT cells fixed with 0.5% PFA at 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).

      Flow cytometry: 10μL of conjugate per test.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenCytoskeletal proteins from cultured HeLa cells
      CloneLP5K
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityThe antibody reacts with cytokeratin 7 which has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. Occasionally staining of blood vessel cell walls, particularly endothelial cells, may be observed.

      FUSION PARTNER: Sp2/0
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.
      Gene Symbol
      • KRT7
      • Sarcolectin
      • Keratin-7
      • K7
      • MGC3625
      • CK-7
      • MGC129731
      • SCL
      • Cytokeratin-7
      • K2C7
      • CK7
      • sarcolectin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08729 # Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7).
      SIZE: 469 amino acids; 51418 Da
      SUBUNIT: Heterotetramer of two type I and two type II keratins. Interacts with eukaryotic translation initiator factor 3 (eIF3) subunit EIF3S10 and with HPV16 E7.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
      PTM: Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
      SIMILARITY: SwissProt: P08729 ## Belongs to the intermediate filament family.
      MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceTested
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C for up to one year.
      Packaging Information
      Material Size25 assays
      Transport Information
      Supplemental Information
      Specifications