07-1229 | Anti-DAPK2 Antibody

07-1229
100 µg  
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      Overview

      Replacement Information
      Replacement Information07-1229 is a recommended replacement for AB3606.

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1229
      ReplacesAB3606
      DescriptionAnti-DAPK2 Antibody
      Alternate Names
      • DAP kinase
      • DAP- kinase-related protein
      • Death-associated protein kinase
      Background InformationDeath Associated Protein Kinase (DAPK) is a Ca2+/Calmodulin-dependent, cytoskeletal associated protein kinase whose expression is implicated in the sensitivity of cells to apoptotic effects of TNFα and Interferon-γ. Deletion of the Calmodulin-binding domain results in a constitutively active mutant with a more potent cytotoxic effect. In contrast, the catalytically inactive mutant exerts a dominant negative effect, reducing cytotoxicity and protecting cells from Interferon-γ induced cell death. DAPK expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlate with high rates of metastasis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • C2C12 cell lysate
      PresentationPurified rabbit polyclonal IgG in buffer containing PBS and 0.02% sodium azide.
      Applications
      ApplicationDetect DAPK2 using this Anti-DAPK2 Antibody validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemisty (paraffin): DAPK2 representative staining pattern/morphology in apoptotic bodies in colon cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.

      Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.
      Biological Information
      ImmunogenPeptide corresponding to aa 356-370 (ARRKALHPRRRSSTS) of human DAPK2, which is identical to the sequence of mouse origin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityDetects a 42 kDa band by western blot, corresponding to DAPK2. Does not cross-react with DAPK1.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites.
      Gene Symbol
      • DAPK2
      • DRP-1
      • MGC119312
      • EC 2.7.11.1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UIK4 # Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
      COFACTOR: Magnesium.
      SIZE: 370 amino acids; 42898 Da
      SUBUNIT: Homodimer. Homodimerization is required for apoptotic function and is inhibited by autophosphorylation at Ser-318.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.
      SIMILARITY: SwissProt: Q9UIK4 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. & Contains 1 protein kinase domain. ...
      Molecular WeightDetects a 42 kDa band by western blot.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on C2C12 lysates.

      Western Blot Analysis:
      1:500-1,1000 dilution of this lot detected DAPK2 on 10 μg of C2C12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8° C from date of receiept.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-DAPK2 - 2459565 2459565
      Anti-DAPK2 - 1990700 1990700
      Anti-DAPK2 - NG1554810 NG1554810
      Anti-DAPK2 - NG1750557 NG1750557
      Anti-DAPK2 -2785814 2785814

      References

      Reference overviewApplicationPub Med ID
      PIAS3 activates the intrinsic apoptotic pathway in non-small cell lung cancer cells independent of p53 status.
      Dabir, S; Kluge, A; McColl, K; Liu, Y; Lam, M; Halmos, B; Wildey, G; Dowlati, A
      International journal of cancer. Journal international du cancer  134  1045-54  2014

      Show Abstract
      Western Blotting23959540 23959540
      Death-associated protein kinase-related protein 1, a novel serine/threonine kinase involved in apoptosis.
      Inbal, B, et al.
      Mol. Cell. Biol., 20: 1044-54 (2000)  2000

      10629061 10629061
      Death-associated protein kinase 2 is a new calcium/calmodulin-dependent protein kinase that signals apoptosis through its catalytic activity.
      Kawai, T, et al.
      Oncogene, 18: 3471-80 (1999)  1999

      Show Abstract
      10376525 10376525