05-104 | Anti-EGFR Antibody, clone LA22

05-104
250 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-104
      Replaces04-337; 04-338
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-EGFR Antibody, clone LA22
      Alternate Names
      • Receptor tyrosine-protein kinase ErbB-1
      • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog
      • cell growth inhibiting protein 40
      • cell proliferation-inducing protein 61
      • epidermal growth factor receptor
      • epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog)
      • epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog
      • avian)
      Background InformationProtein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
      References
      Product Information
      FormatPurified
      Control
      • Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified mouse monoclonal IgG2a in storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide). Frozen solution.
      Applications
      ApplicationAnti-EGFR Antibody, clone LA22 is an antibody against EGFR for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      4 μg of a previous lot immunoprecipitated EGF receptor from 500 μg of A431 RIPA lysate.

      Immunocytochemistry:
      5 μg/mL of previous lots of the antibody showed positive immunostaining for EGF receptor in A431 cells fixed with 4% paraformaldehyde followed by 100% methanol.
      Biological Information
      ImmunogenA431 membrane proteins
      CloneLA22
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes the sequence of Ala351-Asp364 of human EGF receptor, Mr 180kDa, (based on numbering without the signal peptide) (Wu et al., 1989).
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq].
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluatd by RIPA lysates from human A431 cells under reducing conditions.

      Western Blot Analysis:
      0.5-2 μg/mL of this antibody detected EGF receptor in RIPA lysates from human A431 cells under reducing conditions.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size250 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-EGFR, clone LA22 - 15607 15607
      Anti-EGFR, clone LA22 - 19220 19220
      Anti-EGFR, clone LA22 - 2032250 2032250
      Anti-EGFR, clone LA22 - 21283 21283
      Anti-EGFR, clone LA22 - 2273889 2273889
      Anti-EGFR, clone LA22 - 23112 23112
      Anti-EGFR, clone LA22 - 23676 23676
      Anti-EGFR, clone LA22 - 31568 31568
      Anti-EGFR, clone LA22 - DAM1522943 DAM1522943
      Anti-EGFR, clone LA22 - DAM1661022 DAM1661022

      References

      Reference overviewApplicationPub Med ID
      Efficacy of aerosol therapy of lung cancer correlates with EGFR paralysis induced by AvidinOX-anchored biotinylated Cetuximab.
      De Santis, R; Rosi, A; Anastasi, AM; Chiapparino, C; Albertoni, C; Leoni, B; Pelliccia, A; Santapaola, D; Carollo, V; Marra, E; Aurisicchio, L; Arseni, B; Pacello, ML; Palmieri, G; Battella, S; Petronzelli, F; Milazzo, FM
      Oncotarget  5  9239-55  2014

      Show Abstract
      Western Blotting25238453 25238453
      Glioma surgical aspirate: a viable source of tumor tissue for experimental research.
      Day, BW; Stringer, BW; Wilson, J; Jeffree, RL; Jamieson, PR; Ensbey, KS; Bruce, ZC; Inglis, P; Allan, S; Winter, C; Tollesson, G; Campbell, S; Lucas, P; Findlay, W; Kadrian, D; Johnson, D; Robertson, T; Johns, TG; Bartlett, PF; Osborne, GW; Boyd, AW
      Cancers  5  357-71  2013

      Show Abstract
      Immunohistochemistry24216981 24216981
      Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells.
      Khan IH, Zhao J, Ghosh P, Ziman M, Sweeney C, Kung HJ, Luciw PA
      Assay Drug Dev Technol  8  27-36.  2010

      Show Abstract
      20035613 20035613
      RNA interference screen identifies Usp18 as a regulator of epidermal growth factor receptor synthesis.
      Duex, JE; Sorkin, A
      Molecular biology of the cell  20  1833-44  2009

      Show Abstract Full Text Article
      19158387 19158387
      Enhanced estrogen receptor (ER) alpha, ERBB2, and MAPK signal transduction pathways operate during the adaptation of MCF-7 cells to long term estrogen deprivation.
      Martin, Lesley-Ann, et al.
      J. Biol. Chem., 278: 30458-68 (2003)  2003

      Show Abstract
      Immunoblotting (Western)12775708 12775708
      Aging-related attenuation of EGF receptor signaling is mediated in part by increased protein tyrosine phosphatase activity.
      Kien T Tran, S Diana Rusu, Latha Satish, Alan Wells
      Experimental cell research  289  359-67  2003

      Show Abstract
      14499637 14499637
      Endocytosis of epidermal growth factor receptor regulated by Grb2-mediated recruitment of the Rab5 GTPase-activating protein RN-tre.
      Martinu, L; Santiago-Walker, A; Qi, H; Chou, MM
      The Journal of biological chemistry  277  50996-1002  2002

      Show Abstract
      Immunofluorescence12399475 12399475
      Epidermal growth factor receptor induced apoptosis: potentiation by inhibition of Ras signaling.
      T Högnason, S Chatterjee, T Vartanian, R R Ratan, K M Ernewein, A A Habib, T Högnason, S Chatterjee, T Vartanian, R R Ratan, K M Ernewein, A A Habib
      FEBS letters  491  9-15  2001

      Show Abstract
      11226409 11226409
      Peroxynitrite targets the epidermal growth factor receptor, Raf-1, and MEK independently to activate MAPK.
      Zhang, P, et al.
      J. Biol. Chem., 275: 22479-86 (2000)  2000

      Show Abstract
      Immunoblotting (Western)10801894 10801894
      A differential requirement for the COOH-terminal region of the epidermal growth factor (EGF) receptor in amphiregulin and EGF mitogenic signaling
      Wong, L., et al
      J Biol Chem, 274:8900-9 (1999)  1999

      Immunoblotting (Western)10085134 10085134

      Brochure

      Title
      Western Blotting Tools

      FAQ

      QuestionAnswer
      How do I prepare a non-reducing sample buffer?Non-reducing sample buffer can be made and stored for months at room temperature. Here is the formulation for a 2X Non-reducing sample buffer: Water (37ml), 0.5M Tris-HCl (10ml), Glycerol (8ml), 10% SDS (16ml), 0.05% Bromophenol Blue (5ml).

      Related Products & Applications

      Related Products

      Included Positive Control

      Catalogue Number Description  
      12-301 Non-Stimulated A431 Cell Lysate Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies