04-290 | Anti-EGFR (CT) Antibody, clone 13G8

04-290
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MELISA, ICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-290
      Replaces04-337; 04-338
      Brand Family Chemicon®
      Trade Name
      • Upstate
      DescriptionAnti-EGFR (CT) Antibody, clone 13G8
      Alternate Names
      • ERBB
      • mENA
      • ERBB1
      References
      Product Information
      FormatPurified
      Control
      • Includes EGF treated Hep2G lysate as a positive control
      Presentation100 µg of purified mouse monoclonal IgG lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose
      Applications
      ApplicationAnti-EGFR (C-terminus) Antibody, clone 13G8 detects level of EGFR (C-terminus) & has been published & validated for use in ELISA, IC & WB.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of the C terminus in human EGFR
      Clone13G8
      HostMouse
      SpecificityRecognizes C-terminus domain of EGF receptor (aa1165-1186)
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Purification MethodSubsequent thiophilic adsorption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-EGFR (C-terminus), clone 13G8 (Mouse monoclonal IgG1) - 2386981 2386981
      Anti-EGFR (C-terminus), clone 13G8 (Mouse monoclonal IgG1) 3008457
      Anti-EGFR (C-terminus), clone 13G8 (Mouse monoclonal IgG1) 2960493
      Anti-EGFR (C-terminus), clone 13G8 (Mouse monoclonal IgG1) - 2343546 2343546
      Anti-EGFR (C-terminus), clone 13G8 - 0007S0401 0007S0401
      Anti-EGFR (C-terminus), clone 13G8 - 1956698 1956698
      Anti-EGFR (C-terminus), clone 13G8 - DAM1493427 DAM1493427
      Anti-EGFR (C-terminus), clone 13G8 - DAM1574340 DAM1574340
      Anti-EGFR (C-terminus), clone 13G8 - DAM1639213 DAM1639213
      Anti-EGFR (C-terminus), clone 13G8 - DAM1644598 DAM1644598

      References

      Reference overviewPub Med ID
      The angiogenic factor angiopoietin-1 is a proneurogenic peptide on subventricular zone stem/progenitor cells.
      Rosa, AI; Gonçalves, J; Cortes, L; Bernardino, L; Malva, JO; Agasse, F
      The Journal of neuroscience : the official journal of the Society for Neuroscience  30  4573-84  2010

      Show Abstract
      20357108 20357108
      Identification of a novel EGF-sensitive cell cycle checkpoint
      Walker, Francesca, et al
      Exp Cell Res, 313:511-26 (2007)  2007

      17157295 17157295
      Somatic mutations of the epidermal growth factor receptor and non-small-cell lung cancer.
      Zhang, Xiaozhu and Chang, Alex
      J. Med. Genet., 44: 166-72 (2007)  2007

      Show Abstract
      17158592 17158592
      Gefitinib induces premature senescence in non-small cell lung cancer cells with or without EGFR gene mutation.
      Hotta, Katsuyuki, et al.
      Oncol. Rep., 17: 313-7 (2007)  2007

      Show Abstract
      17203166 17203166

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