07-662 | Anti-Estrogen-related Receptor α Antibody

07-662
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ht, M, REMSA, IHC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-662
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Estrogen-related Receptor α Antibody
      OverviewEstrogen-related receptor alpha is a nuclear receptor protein related to the Estrogen receptor. It is reported to interact with estrogen and similar steroids and functions as a transcriptional activator.
      Background InformationThis protein is a nuclear receptor that is closely related to the estrogen receptor. This protein acts as a site specific transcription regulator and has been also shown to interact with estrogen and the transcripton factor TFIIB by direct protein-protein contact. The binding and regulatory activities of this protein have been demonstrated in the regulation of a variety of genes including lactoferrin, osteopontin, medium chain acyl coenzyme A dehydrogenase (MCAD) and thyroid hormone receptor genes. In mouse, ERR alpha is expressed in many adult and embryonic tissues (particularly at the onset of ossification) as well as in several osteoblast cell lines. ERR alpha expression has been documented in mouse in brain, spinal cord, pituitary gland and heart.
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: HeLa Nuclear Extract (12-309).
      PresentationProtein A Purified rabbit IgG in a solution of 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.135M NaCl, and 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-Estrogen-related Receptor α Antibody is a Rabbit Polyclonal Antibody for detection of Estrogen-related Receptor α also known as ERR alpha & has been tested in EMSA, IHC, IP & WB.
      Key Applications
      • Electrophoretic Mobility Shift Assay
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenOvalbumin-conjugated, synthetic peptide corresponding to amino acids 339-364 of human Estrogen Receptor-Related α
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Estrogen-related Receptor α.
      IsotypeIgG
      Species Reactivity
      • Human
      • Hamster
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
      Gene Symbol
      • ESR1
      • ER-alpha
      • ESR
      • Era
      • ER
      • NR3A1
      • ESRA
      • DKFZp686N23123
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
      SIZE: 595 amino acids; 66216 Da
      SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1.
      SUBCELLULAR LOCATION: Nucleus.
      DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
      PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
      MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.
      Molecular Weight53 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on RIPA lysates from HeLa nuclear extracts.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Estrogen Receptor α (rabbit polyclonal IgG) Q2922233
      Anti-Estrogen Receptor-R (rabbit polyclonal IgG) - 1993866 1993866
      Anti-Estrogen Receptor-R (rabbit polyclonal IgG) - 2290948 2290948
      Anti-Estrogen Receptor-R - 2178424 2178424
      Anti-Estrogen Receptor-R - JBC1881348 JBC1881348
      Anti-Estrogen Receptor-R #945; - JBC1863372 JBC1863372
      Anti-Estrogen Receptor-R α (rabbit polyclonal IgG) 3033498
      Anti-Estrogen Receptor-R α (rabbit polyclonal IgG) 3103637
      Anti-Estrogen Receptor-R α (rabbit polyclonal IgG) -2500517 2500517
      Anti-Estrogen Receptor-R α - 2601675 2601675

      References

      Reference overviewApplicationPub Med ID
      Additive effects of clofibric acid and pyruvate dehydrogenase kinase isoenzyme 4 (PDK4) deficiency on hepatic steatosis in mice fed a high saturated fat diet.
      Hwang, B; Wu, P; Harris, RA
      The FEBS journal  279  1883-93  2012

      Show Abstract
      22429297 22429297
      MiR-137 targets estrogen-related receptor alpha and impairs the proliferative and migratory capacity of breast cancer cells.
      Zhao, Y; Li, Y; Lou, G; Zhao, L; Xu, Z; Zhang, Y; He, F
      PloS one  7  e39102  2012

      Show Abstract
      Western Blotting22723937 22723937
      A novel steroidal inhibitor of estrogen-related receptor alpha (ERRalpha).
      Duellman SJ, Calaoagan JM, Sato BG, Fine R, Klebansky B, Chao WR, Hobbs P, Collins N, Sambucetti L, Laderoute KR
      Biochem Pharmacol  80  819-826. Epub 2010 May 31.  2010

      Show Abstract
      20513360 20513360
      Phosphorylation-dependent sumoylation regulates estrogen-related receptor-alpha and -gamma transcriptional activity through a synergy control motif.
      Tremblay, AM; Wilson, BJ; Yang, XJ; Giguère, V
      Molecular endocrinology (Baltimore, Md.)  22  570-84  2008

      Show Abstract
      Western Blotting18063693 18063693
      The mouse estrogen receptor-related orphan receptor alpha 1: molecular cloning and estrogen responsiveness.
      Shigeta, H, et al.
      J. Mol. Endocrinol., 19: 299-309 (1997)  1997

      Show Abstract
      9460651 9460651

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      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies