06-711 | Anti-FADD Antibody

06-711
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIH(P), WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-711
      Replaces04-1113
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FADD Antibody
      Alternate Names
      • FAS-associating death domain-containing protein
      • Growth-inhibiting gene 3 protein
      • Mediator of receptor induced toxicity
      • Protein FADD
      Background InformationFAS-associated death domain protein (UniProt: Q13158; also known as FAS-associating death domain-containing protein, Growth-inhibiting gene 3 protein, Mediator of receptor induced toxicity, Protein FADD) is encoded by the FADD (also known as MORT1, GIG3) gene (Gene ID: 8772) in human. FADD is an apoptotic adaptor molecule that bridges death receptor signaling to the caspase cascade. It is expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes. FADD contains a death domain at its C-terminus and a death effector domain (DED) at its N-terminal. The death domain is responsible for FADD binding to the activated trimerized Fas receptor. FADD recruits procaspase-8 through its N-terminal DED, which is then activated following the cleavage of pro domain. The death inducing signaling complex (DISC) formed by Fas receptor, FADD, and caspase-8 is essential in triggering apoptotic events in cells. Human FADD is phosphorylated mainly at Ser194. Defects in FADD gene have been linked to recurrent infections, hepatic dysfunction, and cardiovascular malformations. These subjects display high circulating levels of CD4(-) and CD8(-) cells and elevated levels of IL-10.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationDetect FAS-associated death domain protein using this rabbit polyclonal Anti-FADD, Cat. No. 06-711. It has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution of this antibody detected FADD in human pancreas, human kidney, and human prostate tissue.
      Biological Information
      ImmunogenHis-tagged full-length human recombinant FAS-associated death domain protein.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects FADD protein in human cells.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FADD
      • GIG3
      • MGC8528
      • MORT1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      Molecular Weight~26 kDa observed; 23.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected FADD in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FADD (28kDa) (rabbit polyclonal IgG) - 2106962 2106962
      Anti-FADD (28kDa) (rabbit polyclonal IgG) - 2375558 2375558
      Anti-FADD (28kDa) - 1957282 1957282
      Anti-FADD (28kDa) - 2536517 2536517
      Anti-FADD (28kDa) - DAM1487553 DAM1487553
      Anti-FADD (28kDa) - JBC1884537 JBC1884537
      Anti-FADD (28kDa) -2507234 2507234
      Anti-FADD (28kDa) -2606788 2606788
      Anti-FADD - 16673 16673
      Anti-FADD - JBC1353434 JBC1353434

      References

      Reference overviewApplicationPub Med ID
      PCTAIRE1-knockdown sensitizes cancer cells to TNF family cytokines.
      Yanagi, T; Shi, R; Aza-Blanc, P; Reed, JC; Matsuzawa, S
      PloS one  10  e0119404  2015

      Show Abstract
      25790448 25790448
      Novel phosphorylation and ubiquitination sites regulate reactive oxygen species-dependent degradation of anti-apoptotic c-FLIP protein.
      Wilkie-Grantham, RP; Matsuzawa, S; Reed, JC
      The Journal of biological chemistry  288  12777-90  2013

      Show Abstract
      Western Blotting23519470 23519470
      Akt-phosphorylated mitogen-activated kinase-activating death domain protein (MADD) inhibits TRAIL-induced apoptosis by blocking Fas-associated death domain (FADD) association with death receptor 4.
      Li, P; Jayarama, S; Ganesh, L; Mordi, D; Carr, R; Kanteti, P; Hay, N; Prabhakar, BS
      The Journal of biological chemistry  285  22713-22  2010

      Show Abstract Full Text Article
      20484047 20484047
      Molecular determinants of kinase pathway activation by Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand.
      Varfolomeev, E; Maecker, H; Sharp, D; Lawrence, D; Renz, M; Vucic, D; Ashkenazi, A
      The Journal of biological chemistry  280  40599-608  2005

      Show Abstract
      16227629 16227629
      JNK regulates HIPK3 expression and promotes resistance to Fas-mediated apoptosis in DU 145 prostate carcinoma cells.
      Curtin, James F and Cotter, Thomas G
      J. Biol. Chem., 279: 17090-100 (2004)  2004

      Show Abstract
      Immunoprecipitation14766760 14766760
      FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis.
      Chinnaiyan, A M, et al.
      Cell, 81: 505-12 (1995)  1995

      Show Abstract
      7538907 7538907

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies