05-486 | Anti-FADD Antibody, clone 1F7

05-486
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-486
      Replaces04-1113
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FADD Antibody, clone 1F7
      References
      Product Information
      FormatPurified
      PresentationPBS, pH 7.2 and 50% glycerol
      Applications
      ApplicationAnti-FADD Antibody, clone 1F7 detects level of FADD & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged full-length fusion protein corresponding to human FADD
      Cloneclone 1F7
      HostMouse
      SpecificityFADD
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development.
      Gene Symbol
      • FADD
      • GIG3
      • MGC8528
      • MORT1
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13158 # Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis.
      SIZE: 208 amino acids; 23279 Da
      SUBUNIT: Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and LRDD.
      TISSUE SPECIFICITY: Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.
      DOMAIN:SwissProt: Q13158 Contains a death domain involved in the binding of the corresponding domain within Fas receptor.
      PTM: Phosphorylated.
      SIMILARITY: Contains 1 death domain. & Contains 1 DED (death effector) domain.
      Molecular Weight28kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from Jurkat cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FADD, clone 1F7 (mouse monoclonal IgG1) 2896338
      Anti-FADD, clone 1F7 (mouse monoclonal IgG1) 2884702
      Anti-FADD, clone 1F7 (mouse monoclonal IgG1) - 2026376 2026376
      Anti-FADD, clone 1F7 (mouse monoclonal IgG1) - 2070384 2070384
      Anti-FADD, clone 1F7 (mouse monoclonal IgG1) - 2262609 2262609
      Anti-FADD, clone 1F7 (mouse monoclonal IgG1) - 2315704 2315704
      Anti-FADD, clone 1F7 (mouse monoclonal IgG1) - 2330488 2330488
      Anti-FADD, clone 1F7 (mouse monoclonal IgG1) - DAM1472189 DAM1472189
      Anti-FADD, clone 1F7 - 17128 17128
      Anti-FADD, clone 1F7 - 18290 18290

      References

      Reference overviewApplicationPub Med ID
      Identification of a novel Bcl-2-interacting mediator of cell death (Bim) E3 ligase, tripartite motif-containing protein 2 (TRIM2), and its role in rapid ischemic tolerance-induced neuroprotection.
      Thompson, Simon, et al.
      J. Biol. Chem., 286: 19331-9 (2011)  2011

      Show Abstract
      21478148 21478148
      Suppression of TNF receptor-1 signaling in an in vitro model of epileptic tolerance.
      Thompson, SJ; Ashley, MD; Stöhr, S; Schindler, C; Li, M; McCarthy-Culpepper, KA; Pearson, AN; Xiong, ZG; Simon, RP; Henshall, DC; Meller, R
      International journal of physiology, pathophysiology and pharmacology  3  120-32  2011

      Show Abstract Full Text Article
      21760970 21760970
      JNK regulates HIPK3 expression and promotes resistance to Fas-mediated apoptosis in DU 145 prostate carcinoma cells.
      Curtin, James F and Cotter, Thomas G
      J. Biol. Chem., 279: 17090-100 (2004)  2004

      Show Abstract
      Immunoprecipitation14766760 14766760
      Death domain mutagenesis of KILLER/DR5 reveals residues critical for apoptotic signaling
      McDonald, E. R., 3rd, et al
      J Biol Chem, 276:14939-45 (2001)  2001

      Immunoblotting (Western)11279061 11279061
      FADD, a novel death domain-containing protein, interacts with the death domain of Fas and initiates apoptosis.
      Chinnaiyan, A M, et al.
      Cell, 81: 505-12 (1995)  1995

      Show Abstract
      7538907 7538907

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies