06-543 | Anti-FAK Antibody

06-543
200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, HtWB, IP, ICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-543
      Replaces04-591
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FAK Antibody
      Alternate Names
      • FADK 1
      • PTK2 protein tyrosine kinase 2
      • Protein-tyrosine kinase 2
      • focal adhesion kinase 1
      Background InformationFocal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and as a key element of integrin signaling. FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. FAK and it's phosphorylation states have been implicated in cancer metastasis and tumor cell survival and adhesion-independent growth. Additionally, recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential. A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.
      References
      Product Information
      FormatPurified
      Control
      • Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
      PresentationPurified rabbit polyclonal IgG in buffer containing storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide). Store at -20°C.
      Applications
      ApplicationThis Anti-FAK Antibody is validated for use in WB, IP, IC for the detection of FAK.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesImmunocytochemistry: 10 μg/mL of a previous lot showed positive immunostaining for Fak in Ref 52 cells fixed with 3.7% formaldehyde.

      Immunoprecipitation: 4 μg of a previous lot immunoprecipitated FAK from 500 μg of murine 3T3/A31 RIPA lysate.
      Biological Information
      ImmunogenpGEX-derived fusion protein containing residues 748-1053 of human FAK
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Hamster
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. At least four transcript variants encoding four different isoforms have been found for this gene, but the full-length natures of only two of them have been determined.
      Gene Symbol
      • PTK2
      • pp125FAK
      • FADK
      • FAK1
      • FAK
      Non-Reactive Species
      • Avian
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q05397 # Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
      SIZE: 1052 amino acids; 119233 Da
      SUBUNIT: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with SHB (By similarity). Interacts with TGFB1I1.
      SUBCELLULAR LOCATION: Cell junction, focal adhesion; Cytoplasmic side. Note=Constituent of focal adhesions.
      TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
      DOMAIN:SwissProt: Q05397 The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL (By similarity). & The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
      PTM: Phosphorylated on 6 tyrosine residues upon activation.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily. & Contains 1 FERM domain. & Contains 1 protein kinase domain.
      Molecular Weight125 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysates from murine 3T3/A31 or human A431 RIPA cell lysate.

      Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FAK in RIPA lysates from murine 3T3/A31 and previously in human A431 RIPA cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FAK - 2391020 2391020
      Anti-FAK - 2436397 2436397
      Anti-FAK - 16334 16334
      Anti-FAK - 18089 18089
      Anti-FAK - 18623 18623
      Anti-FAK - 1957283 1957283
      Anti-FAK - 19725 19725
      Anti-FAK - 2040537 2040537
      Anti-FAK - 21819 21819
      Anti-FAK - 2300469 2300469

      References

      Reference overviewApplicationSpeciesPub Med ID
      FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth.
      Tancioni, I; Miller, NL; Uryu, S; Lawson, C; Jean, C; Chen, XL; Kleinschmidt, EG; Schlaepfer, DD
      Breast cancer research : BCR  17  47  2015

      Show Abstract
      25880415 25880415
      Intracellular modifiers of integrin alpha 6p production in aggressive prostate and breast cancer cell lines.
      Kacsinta, AD; Rubenstein, CS; Sroka, IC; Pawar, S; Gard, JM; Nagle, RB; Cress, AE
      Biochemical and biophysical research communications  454  335-40  2014

      Show Abstract
      25450398 25450398
      N-wasp is required for structural integrity of the blood-testis barrier.
      Xiao, X; Mruk, DD; Tang, EI; Massarwa, R; Mok, KW; Li, N; Wong, CK; Lee, WM; Snapper, SB; Shilo, BZ; Schejter, ED; Cheng, CY
      PLoS genetics  10  e1004447  2014

      Show Abstract
      24967734 24967734
      Absence of γ-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle.
      Moorwood, C; Philippou, A; Spinazzola, J; Keyser, B; Macarak, EJ; Barton, ER
      Skeletal muscle  4  13  2014

      Show Abstract
      25024843 25024843
      Genetic removal of matrix metalloproteinase 9 rescues the symptoms of fragile X syndrome in a mouse model.
      Sidhu, H; Dansie, LE; Hickmott, PW; Ethell, DW; Ethell, IM
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  9867-79  2014

      Show Abstract
      Immunofluorescence25057190 25057190
      Intercellular adhesion molecule-2 is involved in apical ectoplasmic specialization dynamics during spermatogenesis in the rat.
      Xiao, X; Cheng, CY; Mruk, DD
      The Journal of endocrinology  216  73-86  2013

      Show Abstract
      23097088 23097088
      p-FAK-Tyr(397) regulates spermatid adhesion in the rat testis via its effects on F-actin organization at the ectoplasmic specialization.
      Wan, HT; Mruk, DD; Li, SY; Mok, KW; Lee, WM; Wong, CK; Cheng, CY
      American journal of physiology. Endocrinology and metabolism  305  E687-99  2013

      Show Abstract
      Western BlottingRat23880313 23880313
      c-Yes regulates cell adhesion at the apical ectoplasmic specialization-blood-testis barrier axis via its effects on protein recruitment and distribution.
      Xiao, X; Mruk, DD; Cheng, CY
      American journal of physiology. Endocrinology and metabolism  304  E145-59  2013

      Show Abstract
      Western BlottingRat23169788 23169788
      S-Glutathionylation of LMW-PTP regulates VEGF-mediated FAK activation and endothelial cell migration.
      Abdelsaid, Mohammed A and El-Remessy, Azza B
      J. Cell. Sci., (2012)  2012

      Show Abstract
      22854047 22854047
      Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics.
      Ferrando, IM; Chaerkady, R; Zhong, J; Molina, H; Jacob, HK; Herbst-Robinson, K; Dancy, BM; Katju, V; Bose, R; Zhang, J; Pandey, A; Cole, PA
      Molecular & cellular proteomics : MCP  11  355-69  2012

      Show Abstract
      Western Blotting22499769 22499769

      Related Products & Applications

      Related Products

      Included Positive Control

      Catalogue Number Description  
      12-305 3T3/A31 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies