04-1007 Anti-FOXO3a Antibody, clone EP1949Y, rabbit monoclonal

04-1007
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICC, IH(P)RbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1007
      DescriptionAnti-FOXO3a Antibody, clone EP1949Y, rabbit monoclonal
      Alternate Names
      • AF6q21 protein
      • Forkhead in rhabdomyosarcoma-like 1
      • forkhead box O3
      • forkhead box O3A
      • forkhead homolog (rhabdomyosarcoma) like 1
      • forkhead
      • Drosophila
      • homolog of
      • in rhabdomyosarcoma-like 1
      Background InformationForkhead box O3A (FOXO3A) is a member of the forkhead family of transcription factors (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. Upon oxidative stress, FOXO3A translocates to the nucleus where binding to the DNA sequence 5’-[AG]TAAA[TC]A-3’ occurs and IGF-1 is dephosphlorated, resulting in apoptosis. In order for the cell to survive, IGF-1 must be phosphlorated by AKT1/PKB at Thr32 and Ser-253, which will result in retention and inactivation of FOXO3A in the cytoplasm. Translocation of FOXO3A with the MLL gene has been associated with secondary acute leukemia.
      References
      Product Information
      FormatUnpurified
      Control
      • Jurkat cell lysate
      PresentationRabbit Monoclonal in buffer containing sodium azide, glycerol, and BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected FOXO3a in human breast carcinoma tissue.
      Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues near the N-terminus of human FOXO3a.
      EpitopeN-terminus
      CloneEP1949Y
      HostRabbit
      SpecificityThis antibody recognizes FOXO3a at and around the N-terminus.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
      Gene Symbol
      • AF6q21
      • DKFZp781A0677
      • FKHRL1
      • FKHRL1P2
      • FOXO2
      • FOXO3A
      • MGC12739
      • MGC31925
      • OTTHUMP00000016944
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'.
      SUBUNIT STURCTURE: Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation.
      SUBCELLULAR LOCATION: Cytoplasm › cytosol. Nucleus. Note: Translocates to the nucleus upon oxidative stress and in the absence of survival factors.
      TISSUE SPECIFICITY: Ubiquitous.
      PTM: In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation.
      INVOLVEMENT IN DISEASE: A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.
      SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain.
      Molecular Weight~90 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on Jurkat cell lysates.
      Western Blot Analysis: A 1:2,000-5,000 dilution of this antibody was used to detect FOXO3a in Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-FOXO3a, clone EP1949Y, Rabbit Monoclonal - 21200672120067
      Anti-FOXO3a, clone EP1949Y, Rabbit Monoclonal - 23618812361881
      Anti-FOXO3a, clone EP1949Y, Rabbit Monoclonal - 24284982428498
      Anti-FOXO3a, clone EP1949Y, Rabbit Monoclonal - 21530662153066
      Anti-FOXO3a, clone EP1949Y, -25913532591353
      Anti-FOXO3a, clone EP1949Y, Rabbit Monoclonal - 20730622073062
      Anti-FOXO3a, clone EP1949Y, Rabbit Monoclonal - 21085732108573
      Anti-FOXO3a, clone EP1949Y, Rabbit Monoclonal - 21960532196053
      Anti-FOXO3a, clone EP1949Y, Rabbit Monoclonal - 22468392246839
      Anti-FOXO3a, clone EP1949Y, Rabbit Monoclonal - NG1628018NG1628018

      References

      Reference overviewPub Med ID
      SREBP-1 transcription factors regulate skeletal muscle cell size by controlling protein synthesis through myogenic regulatory factors.
      Dessalle, K; Euthine, V; Chanon, S; Delarichaudy, J; Fujii, I; Rome, S; Vidal, H; Nemoz, G; Simon, C; Lefai, E
      PloS one  7  e50878  2012

      Show Abstract
      23226416 23226416
      Mechanistic insight into WEB-2170-induced apoptosis in human acute myelogenous leukemia cells: the crucial role of PTEN.
      Cellai, Cristina, et al.
      Exp. Hematol., 37: 1176-1185.e21 (2009)  2009

      Show Abstract
      19615424 19615424

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies