05-201 | Anti-Fas Antibody (human, activating), clone CH11

05-201
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICC, WBMAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue Number05-201
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Fas Antibody (human, activating), clone CH11
      Alternate Names
      • APO-1 cell surface antigen
      • CD95 antigen
      • Fas (TNF receptor superfamily, member 6)
      • Fas AMA
      • Fas antigen
      • apoptosis antigen 1
      • tumor necrosis factor receptor superfamily, member 6
      Background InformationFas/APO-1/CD95 (36 kDa) is a member of the tumor necrosis factor (TNF) receptor superfamily, a family of transmembrane receptors. Fas has been shown to be an important mediator of apoptotic cell death, as well as being involved in inflammation. Binding of the Fas ligand (Fas-L) induces trimerization of Fas in the target cell membrane. Activation of Fas causes the recruitment of Fas-associated protein with death domain (FADD) via interactions between the death domains of Fas and FADD. Procaspase 8 binds to Fas-bound FADD via interactions between the death effector domains (DED) of FADD and pro-caspase 8 leading to the activation of caspase 8. Activated caspase 8 cleaves (activates) other procaspases, in effect beginning a caspase cascade that ultimately leads to apoptosis. Caspases cleave nuclear lamins, causing the nucleus to break down and lose its normal structure. Fas-induced apoptosis can be effectively blocked at several stages by either FLICE-inhibitory protein (FLIP), by Bcl-2, or by the cytokine response modifier A (CrmA).

      Biological Activity
      The antibody demonstrates cytolytic activity on human cells that express Fas. Murine WR19L cells and L929 cells transfected with cDNA encoding human Fas undergo apoptosis in response to this antibody.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human liver tumor, human breast tumor or Jurkat whole cell lysate, Raji cell lysate.
      PresentationPurified mouse monoclonal IgM in buffer containing PBS, pH 7.2, with 50% glycerol. Liquid at -20ºC.
      Applications
      ApplicationDetect Fas using this Anti-Fas Antibody (human, activating), clone CH11 has been published and validated for use in Flow Cytometry (FC), Immunocytochemistry (IC) and Western Blot (WB).
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blot:
      0.5-2 μg/mL of a previous lot detected Fas in a Raji cell lysate.
      Immunocytochemistry:
      5-10 μg/mL of a previous lot detected Fas on HeLa cells fixed with 4% formalin/2% acetic acid.
      Flow cytometry:
      A previous lot of was tested by an independent laboratory using 20 μg/mL of anti-Fas, clone CH11 (Yonehara, S., 1989; Kobayashi, N., 1990).
      Biological Information
      ImmunogenFS-7 (human diploid fibroblast cell line). Clone CH-11.
      CloneCH11
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the human cell surface antigen Fas, Mr 43 kDa expressed in various human cells, including myeloid cells, T lympho-blastoid cells, and diploid fibroblasts.
      IsotypeIgM
      Species Reactivity
      • Human
      Species Reactivity NoteProven to react with human.
      This antibody does not recognize TNF, and does not cross-react with mouse Fas. Fas ligand will induce apoptosis in human, mouse and rat systems.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contains a death domain. It has been shown to play a central role in the physiological regulation of programmed cell death, and has been implicated in the pathogenesis of various malignancies and diseases of the immune system. The interaction of this receptor with its ligand allows the formation of a death-inducing signaling complex that includes Fas-associated death domain protein (FADD), caspase 8, and caspase 10. The autoproteolytic processing of the caspases in the complex triggers a downstream caspase cascade, and leads to apoptosis. This receptor has been also shown to activate NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and is found to be involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full length isoform.
      Gene Symbol
      • FAS
      • OTTHUMP00000059646
      • LFG
      • FASTM
      • ALPS1A
      • TNFRSF6
      • CD95
      • FAS1
      • FAIM2
      • APT1
      • NMP35
      • APO-1
      Purification MethodImmunoaffinity Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P25445 # Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro).
      SIZE: 335 amino acids; 37732 Da
      SUBUNIT: Binds DAXX. Interacts with HIPK3. Part of a complex containing HIPK3 and FADD (By similarity). Binds RIPK1 and FAIM2. Interacts with BRE.
      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted. & Isoform 3: Secreted. & Isoform 4: Secreted. & Isoform 5: Secreted. & Isoform 6: Secreted.
      TISSUE SPECIFICITY: Isoform 1 and isoform 6 are expressed at equal levels in resting peripheral blood mononuclear cells. After activation there is an increase in isoform 1 and decrease in the levels of isoform 6.
      DOMAIN: SwissProt: P25445 Contains a death domain involved in the binding of FADD, and maybe to other cytosolic adapter proteins.
      DISEASE: SwissProt: P25445 # Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A) [MIM:601859]; also known as Canale-Smith syndrome (CSS). ALPS is a childhood syndrome involving hemolytic anemia and thrombocytopenia with massive lymphadenopathy and splenomegaly.
      SIMILARITY: Contains 1 death domain. & Contains 3 TNFR-Cys repeats.
      Molecular Weight43 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Quality AssuranceRoutinely evaluated by demonstrating cytolytic activity on human cells that express Fas. Murine WR19L cells and L929 cells transfected with cDNA encoding human Fas undergo apoptosis in response to this antibody.

      Apoptosis Assay Analysis:
      15-20 µg/mL of this lot maximally induced apoptosis of human Jurkat cells with 83% mortality after 24 hours of treatment.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of the product, centrifuge the original vial prior to removing the cap.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Fas (human, activating) 2453649
      Anti-Fas (human, activating), clone CH11 - 2145050 2145050
      Anti-Fas (human, activating), clone CH11 - 2390345 2390345
      Anti-Fas (human, activating), clone CH11 - 2397046 2397046
      Anti-Fas (human, activating), -2552129 2552129
      Anti-Fas (human, activating), -2586497 2586497
      Anti-Fas (human, activating), -2618494 2618494
      Anti-Fas (human, activating), -2626044 2626044
      Anti-Fas (human, activating), -2639454 2639454
      Anti-Fas (human, activating), -2691384 2691384

      References

      Reference overviewApplicationSpeciesPub Med ID
      Iridovirus CARD Protein Inhibits Apoptosis through Intrinsic and Extrinsic Pathways.
      Chen, CW; Wu, MS; Huang, YJ; Lin, PW; Shih, CJ; Lin, FP; Chang, CY
      PloS one  10  e0129071  2015

      Show Abstract
      26047333 26047333
      Cytokine effects on cell viability and death of prostate carcinoma cells.
      Chondrogiannis, G; Kastamoulas, M; Kanavaros, P; Vartholomatos, G; Bai, M; Baltogiannis, D; Sofikitis, N; Arvanitis, D; Galani, V
      BioMed research international  2014  536049  2014

      Show Abstract
      Western Blotting24982891 24982891
      Akt-mediated anti-apoptotic effects of substance P in Anti-Fas-induced apoptosis of human tenocytes.
      Backman, LJ; Danielson, P
      Journal of cellular and molecular medicine  17  723-33  2013

      Show Abstract
      23577779 23577779
      Asymmetric dimethylarginine attenuates serum starvation-induced apoptosis via suppression of the Fas (APO-1/CD95)/JNK (SAPK) pathway.
      Li, H; Zhou, Y; Zhao, A; Qiu, Y; Xie, G; Jiang, Q; Zheng, X; Zhong, W; Sun, X; Zhou, Z; Jia, W
      Cell death & disease  4  e830  2013

      Show Abstract
      24091673 24091673
      IF1 limits the apoptotic-signalling cascade by preventing mitochondrial remodelling.
      Faccenda, D; Tan, CH; Seraphim, A; Duchen, MR; Campanella, M
      Cell death and differentiation  20  686-97  2013

      Show Abstract
      23348567 23348567
      Histone modifications are responsible for decreased Fas expression and apoptosis resistance in fibrotic lung fibroblasts.
      Huang, SK; Scruggs, AM; Donaghy, J; Horowitz, JC; Zaslona, Z; Przybranowski, S; White, ES; Peters-Golden, M
      Cell death & disease  4  e621  2013

      Show Abstract
      Western Blotting23640463 23640463
      Apoptotic DNA degradation into oligonucleosomal fragments, but not apoptotic nuclear morphology, relies on a cytosolic pool of DFF40/CAD endonuclease.
      Iglesias-Guimarais, V; Gil-Guiñon, E; Gabernet, G; García-Belinchón, M; Sánchez-Osuna, M; Casanelles, E; Comella, JX; Yuste, VJ
      The Journal of biological chemistry  287  7766-79  2012

      Show Abstract
      22253444 22253444
      Adenovirus-mediated expression of mutated forkhead human transcription like-1 suppresses tumor growth in a mouse melanoma xenograft model.
      Gomez-Gutierrez, JG; Egger, ME; Hao, H; Zhou, HS; McMasters, KM
      Cancer biology & therapy  13  1195-204  2012

      Show Abstract
      22892845 22892845
      XB130, a novel adaptor protein, promotes thyroid tumor growth.
      Shiozaki, Atsushi, et al.
      Am. J. Pathol., 178: 391-401 (2011)  2011

      Show Abstract
      21224076 21224076
      Development of cell-based high-throughput assays for the identification of inhibitors of receptor activator of nuclear factor-kappa B signaling.
      Ashley, JW; McCoy, EM; Clements, DA; Shi, Z; Chen, T; Feng, X
      Assay and drug development technologies  9  40-9  2011

      Show Abstract
      21050071 21050071

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies