06-727 | Anti-FcεRI Antibody, γ subunit

06-727
200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, FCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-727
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-FcεRI Antibody, γ subunit
      Alternate Names
      • High affinity immunoglobulin epsilon receptor subunit gamma
      • Fc-epsilon RI-gamma
      • Fc receptor gamma-chain
      • FcR gamma
      • IgE Fc receptor subunit gamma
      Background InformationHigh affinity immunoglobulin epsilon receptor subunit gamma (UniProt P20411; also known as Fc-epsilon RI-gamma, Fc receptor gamma-chain, FceRI gamma, FcR gamma, IgE Fc receptor subunit gamma) is encoded by the Fcer1g (also known as Fce1g) gene (Gene ID: 25441) in rat species. FceR1 gamma is a single-pass type I membrane protein that associates with a variety of FcR alpha chains to form a functional signaling complex that regulates several aspects of the immune response. The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface. FceRI gamma is found exclusively on mast cells and basophils. In rodents Fc epsilon RI is a tetrameric complex of non-covalently attached subunits: one IgE-binding alpha subunit, one beta subunit and a dimer of disulfide-linked gamma subunits. When multivalent allergens bind to the receptor-bound IgE, the consequent aggregation of the receptors leads to the release of mediators responsible for allergic symptoms. (Ref.: Blank, U., et al. (1989). Nature 337(6203); 187-189).
      References
      Product Information
      FormatPurified
      Control
      • RBL cell lysate
      PresentationPurified rabbit polylclonal IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol.
      Applications
      ApplicationAnti-FceRI, gamma subunit, Cat. No. 06-727, is a rabbit polyclonal antibody that detects FceRI, subunit and has been tested for use in Flow Cytometry and Western Blotting.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 0.1 µg from a representative lot detected FceRI, gamma subunit in rat basophilic leukemia cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 7 amino acids from the C-terminal region of rat FceRI gamma subunit.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes and is specific for FcεRI, γ-subunit, MW 10kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, rat and mouse. Other species cross-reactivity not tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene SummaryThe IgE receptor plays a central role in allergic disease, coupling allergen and mast cell to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The allergic response occurs when 2 or more high-affinity IgE receptors are crosslinked via IgE molecules that in turn are bound to an allergen (antigen) molecule. A perturbation occurs that brings about the release of histamine and proteases from the granules in the cytoplasm of the mast cell and leads to the synthesis of prostaglandins and leukotrienes--potent effectors of the hypersensitivity response. The IgE receptor consists of 3 subunits: alpha, beta (MIM 147138), and gamma (MIM 147139); only the alpha subunit is glycosylated.
      Gene Symbol
      • FCER1G
      • OTTHUMP00000063478
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P30273 # The gamma subunit has a critical role in allowing the IgE Fc receptor to reach the cell surface.
      SIZE: 86 amino acids; 9667 Da
      SUBUNIT: IgE Fc receptor is a tetramer of an alpha chain, a beta chain, and two disulfide linked gamma chains. The gamma chain from Fc-epsilon-Ri are also subunits of other Fc receptors.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P30273 ## Belongs to the CD3Z/FCER1G family. & Contains 1 ITAM domain.
      Molecular Weight30 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on RIPA lysate of RBL cells.

      Western Blot Analysis: 0.5-2 μg/mL of this antibody detected FcεRI, γ-subunit in RIPA lysate of RBL cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Fcepsilon;RI gamma; subunit - 16409 16409
      Anti-Fcepsilon;RI gamma; subunit - 22781 22781
      Anti-FcepsilonRI, gamma subunit - 29561 29561
      Anti-FcεRI, γ subunit 2474897
      Anti-FcεRI, γ subunit - 2459611 2459611
      Anti-FcεRI, γ subunit -2554079 2554079
      Anti-FcεRI, γ subunit -2554864 2554864
      Anti-FcεRI, γ subunit -2557582 2557582
      Anti-FcεRI, γ subunit -2593887 2593887
      Anti-FcεRI, γ subunit -2654232 2654232

      References

      Reference overviewApplicationPub Med ID
      A role for the ITAM signaling module in specifying cytokine-receptor functions.
      Bezbradica, JS; Rosenstein, RK; DeMarco, RA; Brodsky, I; Medzhitov, R
      Nature immunology  15  333-42  2014

      Show Abstract
      24608040 24608040
      Toll-like receptor 4 and MAIR-II/CLM-4/LMIR2 immunoreceptor regulate VLA-4-mediated inflammatory monocyte migration.
      Totsuka, N; Kim, YG; Kanemaru, K; Niizuma, K; Umemoto, E; Nagai, K; Tahara-Hanaoka, S; Nakahasi-Oda, C; Honda, S; Miyasaka, M; Shibuya, K; Shibuya, A
      Nature communications  5  4710  2014

      Show Abstract
      25134989 25134989
      The response of human macrophages to β-glucans depends on the inflammatory milieu.
      Municio, C; Alvarez, Y; Montero, O; Hugo, E; Rodríguez, M; Domingo, E; Alonso, S; Fernández, N; Crespo, MS
      PloS one  8  e62016  2013

      Show Abstract
      Western Blotting23637950 23637950
      FcγRI mediates serum amyloid P inhibition of fibrocyte differentiation.
      Crawford, JR; Pilling, D; Gomer, RH
      Journal of leukocyte biology  92  699-711  2012

      Show Abstract
      22493081 22493081
      T cells stimulated with an analog peptide of type II collagen require the Fc receptor γ-chain to secrete interleukin-4 and suppress autoimmune arthritis in mice.
      Myers LK, Cullins DL, Brand DD, Kleinau S, Stuart JM, Kang AH.
      Arthritis and rheumatism  63  2661-70  2011

      Show Abstract
      21590683 21590683
      β2 integrin induces TCRζ-Syk-phospholipase C-γ phosphorylation and paxillin-dependent granule polarization in human NK cells.
      March, ME; Long, EO
      Journal of immunology (Baltimore, Md. : 1950)  186  2998-3005  2011

      Show Abstract
      21270398 21270398
      Oxysterol represses high-affinity IgE receptor-stimulated mast cell activation in Liver X receptor-dependent and -independent manners.
      Nunomura S, Endo K, Makishima M, Ra C
      FEBS Lett  584  1143-8 Epub 2010 Feb 9  2010

      Show Abstract
      20138879 20138879
      The human megakaryocytic cell line UT-7/TPO expresses functional platelet agonist signals mediated through GPVI and thromboxane receptor.
      Tatsuya Kawaguchi,Ryuji Hashimoto,Hiroshi Yokota
      Cell biology international  34  2010

      Show Abstract
      20522022 20522022
      Osteoclast inhibitory peptide-1 binding to the Fc gammaRIIB inhibits osteoclast differentiation.
      Shanmugarajan, S; Beeson, CC; Reddy, SV
      Endocrinology  151  4389-99  2010

      Show Abstract
      20610564 20610564
      IgEb immune complexes activate macrophages through FcgammaRIV binding.
      Masayuki Hirano, Randall S Davis, W David Fine, Shugo Nakamura, Kentaro Shimizu, Hirokazu Yagi, Koichi Kato, Robert P Stephan, Max D Cooper
      Nature immunology  8  762-71  2007

      Show Abstract
      17558411 17558411

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      FCABS400F Milli-Mark® Anti-FcεRI Antibody, γ subunit-FITC Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies