05-1139 | Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7

05-1139
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, ChWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1139
      Replaces04-591
      DescriptionAnti-Focal Adhesion Kinase Antibody, clone BLAb2H7
      Alternate Names
      • FADK 1
      • PTK2 protein tyrosine kinase 2
      • Protein-tyrosine kinase 2
      • focal adhesion kinase 1
      Background InformationFAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src (Guan, J L and Shalloway, D). This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton. Additional components of focal adhesions include actin, filamin, vinculin, talin, paxillin and tensin (Burridge, K, et al).
      References
      Product Information
      FormatPurified
      Control
      • HEK293 cell lysate
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7 is validated for use in WB, IP for the detection of Focal Adhesion Kinase.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A previous lot detected Focal Adhesion Kinase at 1:100 dilution in mouse cortex tissue.
      Biological Information
      ImmunogenRecombinant fragment derived from the amino terminal region of chicken FAK, amino acid residues 103 to 553.
      EpitopeTerminal
      CloneBLAb2H7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Focal Adhesion Kinase
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Chicken
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EC 2.7.10.2
      • FADK
      • FAK
      • FAK1
      • pp125FAK
      UniProt Number
      UniProt SummaryFUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
      SUBUNIT STRUCTURE: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB By similarity. Interacts with TGFB1I1 and STEAP4.
      SUBCELLULAR LOCATION: Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions.
      TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
      DOMAIN: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL By similarity.
      The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
      PTM: Phosphorylated on 6 tyrosine residues upon activation.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
      Contains 1 FERM domain.
      Contains 1 protein kinase domain.
      Molecular Weight~125 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.
      Western Blotting Analysis: 0.2 µg/mL of this antibody detected Focal Adhesion Kinase in 10 µg of HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Focal Adhesion Kinase, clone BLAb2H7 - 2433480 2433480
      Anti-Focal Adhesion Kinase, clone BLAb2H7 - 2012969 2012969
      Anti-Focal Adhesion Kinase, clone BLAb2H7 - 2224476 2224476
      Anti-Focal Adhesion Kinase, clone BLAb2H7 - 2527458 2527458
      Anti-Focal Adhesion Kinase, clone BLAb2H7 - 2561092 2561092
      Anti-Focal Adhesion Kinase, clone BLAb2H7 - NG1853791 NG1853791
      Anti-Focal Adhesion Kinase, clone BLAb2H7 - NG1868835 NG1868835
      Anti-Focal Adhesion Kinase, clone BLAb2H7 - NRG1695452 NRG1695452
      Anti-Focal Adhesion Kinase, clone BLAb2H7 Monoclonal Antibody 2975295
      Anti-Focal Adhesion Kinase, clone BLAb2H7 Monoclonal Antibody 2895644

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies