06-756 | Anti-Frizzled 5 Antibody

06-756
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-756
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Frizzled 5 Antibody
      Alternate Names
      • Fz5
      • Fzd5
      Background InformationThe Wnt family consists of ligands involved in cell fate determination, organogenesis, and oncogenesis. The Wnt signal is thought to be transduced sequentially via a homolog of the frizzled family of receptors to GSK-3 (disheveled), β- and γ- catenin and finally to members of the Tcf/Lef-1 family of HMG box transcription factors. Frizzled 5 is believed to function as the receptor for the Wnt5a ligand.
      References
      Product Information
      FormatPurified
      Control
      • Lysates from mouse liver, C2C12, Jurkat, and HUVEC
      PresentationPurified antibody in PBS with 0.05% sodium azide and 30% Glycerol
      Applications
      ApplicationAnti-Frizzled 5 Antibody is an antibody against Frizzled 5 for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide derived from amino acids 564-585 of human Frizzled 5 (CTGRTPPGPAATYHKQVSLSHV).
      EpitopeC-Terminus
      HostRabbit
      SpecificityFrizzled 5 (aka Fz5 or Fzd5)
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMembers of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD5 protein is believed to be the receptor for the Wnt5A ligand.
      Gene Symbol
      • FZD5
      • HFZ5
      • MGC129692
      • hFz5
      • DKFZP434E2135
      • Fz-5
      • Hfz5
      • C2orf31
      • FzE5
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13467 # Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK- 3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta- catenin pathway.
      SIZE: 585 amino acids; 64507 Da
      SUBUNIT: Interacts with GOPC (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Golgi apparatus membrane; Multi-pass membrane protein (By similarity). Note=Localized at the plasma membrane and also found at the Golgi (By similarity).
      DOMAIN: SwissProt: Q13467 The PDZ-binding motif mediates interaction with GOPC (By similarity). & Lys-Thr-X-X-X-Trp motif is involved in the activation of the Wnt/beta-catenin signaling pathway (By similarity). & The FZ domain is involved in binding with Wnt ligands (By similarity).
      SIMILARITY: Belongs to the G-protein coupled receptor Fz/Smo family. & Contains 1 FZ (frizzled) domain.
      Molecular WeightFrizzled 5 (62kDa)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot on lysates from mouse liver, C2C12, Jurkat or HUVEC cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Frizzled 5 - 2441456 2441456
      Anti-Frizzled 5 - 17510 17510
      Anti-Frizzled 5 - 2091903 2091903
      Anti-Frizzled 5 - 2193152 2193152
      Anti-Frizzled 5 - 2343561 2343561
      Anti-Frizzled 5 - DAM1567792 DAM1567792
      Anti-Frizzled 5 - DAM1598787 DAM1598787
      Anti-Frizzled 5 - DAM1764420 DAM1764420
      Anti-Frizzled 5 - DAM1790581 DAM1790581
      Anti-Frizzled 5 - JBC1863318 JBC1863318

      References

      Reference overviewPub Med ID
      WNT-5A stimulates the GDP/GTP exchange at pertussis toxin-sensitive heterotrimeric G proteins.
      Michaela Bc Kilander,Jacomijn P Dijksterhuis,Ranjani Sri Ganji,Vitezslav Bryja,Gunnar Schulte
      Cellular signalling  23  2011

      Show Abstract
      21070854 21070854
      Norrin and frizzled; a new vein for the eye.
      Niehrs, Christof
      Dev. Cell, 6: 453-4 (2004)  2004

      Show Abstract
      15068782 15068782
      The Frizzled family: receptors for multiple signal transduction pathways.
      Huang, Hui-Chuan and Klein, Peter S
      Genome Biol., 5: 234 (2004)  2004

      Show Abstract
      15239825 15239825
      Wnt/beta-catenin signaling pathway as a novel cancer drug target.
      Luu, Hue H, et al.
      Curr Cancer Drug Targets, 4: 653-71 (2004)  2004

      Show Abstract
      15578921 15578921
      Wnt-frizzled signaling in the induction and differentiation of the neural crest.
      Yanfeng, Wang, et al.
      Bioessays, 25: 317-25 (2003)  2003

      Show Abstract
      12655639 12655639
      Interaction of frizzled related protein (FRP) with Wnt ligands and the frizzled receptor suggests alternative mechanisms for FRP inhibition of Wnt signaling.
      Bafico, A, et al.
      J. Biol. Chem., 274: 16180-7 (1999)  1999

      10347172 10347172
      A member of the Frizzled protein family mediating axis induction by Wnt-5A.
      He, X, et al.
      Science, 275: 1652-4 (1997)  1997

      9054360 9054360
      A new member of the frizzled family from Drosophila functions as a Wingless receptor.
      Bhanot, P, et al.
      Nature, 382: 225-30 (1996)  1996

      8717036 8717036
      A large family of putative transmembrane receptors homologous to the product of the Drosophila tissue polarity gene frizzled.
      Wang, Y, et al.
      J. Biol. Chem., 271: 4468-76 (1996)  1996

      8626800 8626800