05-474 | Anti-GABA A Receptor β2/3 Antibody

05-474
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, RWB, IP, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-474
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-GABA A Receptor β2/3 Antibody
      Alternate Names
      • GABA(A) receptor subunit alpha-1
      • gamma-aminobutyric acid (GABA) A receptor, alpha 1
      • gamma-aminobutyric acid A receptor, alpha 1
      Background InformationThe neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 3 different isoforms of the beta subunit, and receptor subunit expression varies during development. Additionally, GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 and beta 2/3 subunits increase upon chronic exposure to alcohol, and may be potential therapeutic targets for alcohol dependency. The beta 2 subunit is required for receptor assembly and surface expression, while mice with an inactivated beta3 subunit exhibit epilepsy and cleft palate.
      References
      Product Information
      FormatPurified
      Control
      • Brain tissue
      PresentationPurified mouse monoclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.
      Applications
      ApplicationAnti-GABA A Receptor β2/3 Antibody, is an antibody against GABA A Receptor β2/3 for use in WB, IP, IH.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunohistochemistry
      Application NotesImmunoprecipitation: 4 μg of a previous lot immunoprecipitated the GABAA Receptor β2 and β3 subunits from 500 μg of rat brain microsomal tissue extract. Due to interference of the IgG heavy chain, the immunoprecipitated GABAA receptor complex was confirmed using a polyclonal anti-GABAA receptor α1 subunit (Catalog # 06-868).
      Immunohistochemistry: This antibody has been reported to detect the GABAA Receptor β2 and β3 subunits in paraffin embedded and frozen rat brain sections which had been fixed with 4% paraformaldehyde or 0.1% glutaraldehyde and permeabilized with Triton X-100.
      Biological Information
      ImmunogenAffinity-purified GABAA receptor isolated from bovine brain. Clone 62-3G1
      Cloneclone 62-3G1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificitySpecific for the GABAA Receptor β2 subunit, MW ~55 kDa and β3 subunit, MW ~57 kDa.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified.
      Gene Symbol
      • GABRA1
      • GABA
      • EJM
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P14867 # GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.
      SIZE: 456 amino acids; 51802 Da
      SUBUNIT: Binds UBQLN1 (By similarity). Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho.
      SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
      DISEASE: SwissProt: P14867 # Defects in GABRA1 are a cause of juvenile myoclonic epilepsy (JME) [MIM:606904]. JME is a common epileptic syndrome characterized by afebrile seizures, onset in adolescence (rather than in childhood) and myoclonic jerks. & Defects in GABRA1 are the cause of childhood absence epilepsy type 4 (ECA4) [MIM:611136]. ECA4 is a subtype of idiopathic generalized epilepsy (IGE) characterized by onset at age 6-7 years, frequent absence seizures (several per day) and bilateral, synchronous, symmetric 3-Hz spike waves on EEG. During adolescence, tonic-clonic and myoclonic seizures develop. Absence seizures may either remit or persist into adulthood.
      SIMILARITY: SwissProt: P14867 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
      Molecular WeightVaries
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on rat brain microsomal preparation.

      Western Blot Analysis: 0.5-2 μg/mL of this antibody detected the GABAA Receptor β2 and β3 subunits from 20 μg of rat brain microsomal preparation (Catalog # 12-144).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2°C to 8°C from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GABA A Receptor #226;2/3, clone 62-3G1 - DAM1687506 DAM1687506
      Anti-GABA A Receptor #946;2/3, clone 62-3G1 - 2123690 2123690
      Anti-GABA A Receptor 2/3, clone 62-3G1 - 1966946 1966946
      Anti-GABA A Receptor 2/3, clone 62-3G1 - 2028682 2028682
      Anti-GABA A Receptor 2/3, clone 62-3G1 - 2196188 2196188
      Anti-GABA A Receptor 2/3, clone 62-3G1 - 2297218 2297218
      Anti-GABA A Receptor 2/3, clone 62-3G1 - DAM1713166 DAM1713166
      Anti-GABA A Receptor 2/3, clone 62-3G1 - DAM1782766 DAM1782766
      Anti-GABA A Receptor 2/3, clone 62-3G1 - DAM1810796 DAM1810796
      Anti-GABA A Receptor 2/3, clone 62-3G1 - JBC1841203 JBC1841203

      References

      Reference overviewApplicationSpeciesPub Med ID
      Cellular plasticity induced by anti-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor encephalitis antibodies.
      Peng, X; Hughes, EG; Moscato, EH; Parsons, TD; Dalmau, J; Balice-Gordon, RJ
      Annals of neurology  77  381-98  2015

      Show Abstract
      25369168 25369168
      Acute mechanisms underlying antibody effects in anti-N-methyl-D-aspartate receptor encephalitis.
      Moscato, EH; Peng, X; Jain, A; Parsons, TD; Dalmau, J; Balice-Gordon, RJ
      Annals of neurology  76  108-19  2014

      Show Abstract
      24916964 24916964
      The ly-6 protein, lynx1, is an endogenous inhibitor of nicotinic signaling in airway epithelium.
      Fu, XW; Rekow, SS; Spindel, ER
      American journal of physiology. Lung cellular and molecular physiology  303  L661-8  2012

      Show Abstract
      22923641 22923641
      Inflammation-induced shift in spinal GABA(A) signaling is associated with a tyrosine kinase-dependent increase in GABA(A) current density in nociceptive afferents.
      Zhu, Y; Dua, S; Gold, MS
      Journal of neurophysiology  108  2581-93  2012

      Show Abstract
      22914654 22914654
      Decreased intracellular GABA levels contribute to spinal cord stimulation-induced analgesia in rats suffering from painful peripheral neuropathy: the role of KCC2 and GABA(A) receptor-mediated inhibition.
      S P Janssen,S Gerard,M E Raijmakers,M Truin,M Van Kleef,E A Joosten
      Neurochemistry international  60  2012

      Show Abstract
      22107704 22107704
      Estrous cycle variations in GABA(A) receptor phosphorylation enable rapid modulation by anabolic androgenic steroids in the medial preoptic area.
      Oberlander, JG; Porter, DM; Onakomaiya, MM; Penatti, CA; Vithlani, M; Moss, SJ; Clark, AS; Henderson, LP
      Neuroscience  226  397-410  2012

      Show Abstract
      22989919 22989919
      Mixed GABA-glycine synapses delineate a specific topography in the nucleus tractus solitarii of adult rat.
      Dufour, A; Tell, F; Kessler, JP; Baude, A
      The Journal of physiology  588  1097-115  2010

      Show Abstract Full Text Article
      ImmunohistochemistryRat20156844 20156844
      Development of gamma-aminobutyric acidergic synapses in cultured hippocampal neurons.
      Swanwick, CC; Murthy, NR; Mtchedlishvili, Z; Sieghart, W; Kapur, J
      The Journal of comparative neurology  495  497-510  2006

      Show Abstract
      16498682 16498682
      Synaptic targeting of PSD-Zip45 (Homer 1c) and its involvement in the synaptic accumulation of F-actin.
      Usui, Shinichi, et al.
      J. Biol. Chem., 278: 10619-28 (2003)  2003

      Show Abstract
      Immunocytochemistry12524440 12524440
      Analgesia and hyperalgesia from GABA-mediated modulation of the cerebral cortex.
      Luc Jasmin, Samuel D Rabkin, Alberto Granato, Abdennacer Boudah, Peter T Ohara
      Nature  424  316-20  2003

      Show Abstract
      12867983 12867983

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies