06-868 | Anti-GABAA Receptor α1 Antibody

06-868
200 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MWB, IH(P), IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-868
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-GABAA Receptor α1 Antibody
      Alternate Names
      • Gamma-amino butyric acid receptor subunit alpha-1
      • GABA(A) receptor subunit alpha-1
      • gamma-aminobutyric acid (GABA) A receptor, alpha 1
      Background InformationThe neurotransmitter GABA (gamma-aminobutyric acid) binds to specific transmembrane receptors in the plasma membrane of both pre- and postsynaptic neurons. Three general classes of GABA receptors are known: GABAA and GABAC (ionotropic) receptors and GABAB (metabotropic) receptors. The GABAA (gamma-Aminobutyric Acid type A) receptor is a multiprotein complex that acts as a ligand-gated chloride channel. These receptors are hetero-oligomers, for which 6 subunits are currently known (a, b, g, d, e and r). There are at least 6 different isoforms of the alpha subunit, and receptor subunit expression varies during development. Increased alpha1 subunit assembly in postsynaptic receptors of cerebellar inhibitory synapses is responsible for the fast inhibitory synaptic currents observed during postnatal development. Additionally, alpha1-containing GABAA receptors are important in regulating the reinforcing properties of alcohol, and expression of the alpha1 subunit increases upon chronic exposure to alcohol, making it a potential therapeutic target for alcohol dependency.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Rat Brain Microsomal Preparation Tissue Lysate.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-GABAA Receptor α1 Antibody is validated for use in IH, IP, WB for the detection of GABAA Receptor α1.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot detected GABAA Receptor α1 in rat hippocampus tissue.

      Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated GABAA Receptor α1 in rat brain microsomal preparation RIPA lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the topological domain of rat GABAA Receptor α1.
      EpitopeTopological domain.
      HostRabbit
      SpecificityThis antibody recognizes the topographical domain of GABAA Receptor α1.
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with Rat and Mouse. Other homologies: Human (94% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySubunit of GABAA receptors, which bind benzodiazepines and act as inhibitory ion channels in the brain.
      Gene Symbol
      • Gabra1
      • Gabra-1
      • GABA
      • EJM
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

      SUBUNIT STRUCTURE: Generally pentameric. There are five types of GABA(A) receptor chains: alpha, beta, gamma, delta, and rho. Binds UBQLN1. Interacts with TRAK1.

      SUBCELLULAR LOCATION: Cell junction > synapse > postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
      Molecular Weight~51 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain microsomal preparation tissue lysate.

      Western Blot Analysis: A 1:10,000 dilution of this antibody detected GABAA Receptor α1 in 10 µg of rat brain microsomal preparation tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GABA<sub>A</sub> Receptor alpha;1 - 17478 17478
      Anti-GABA<sub>A</sub> Receptor alpha;1 - 22589 22589
      Anti-GABAA Receptor #945;1 - 1975358 1975358
      Anti-GABAA Receptor #945;1 - 2153153 2153153
      Anti-GABAA Receptor 1 - 2037871 2037871
      Anti-GABAA Receptor 1 - 2297227 2297227
      Anti-GABAA Receptor 1 - DAM1724041 DAM1724041
      Anti-GABAA Receptor 1 - DAM1764423 DAM1764423
      Anti-GABAA Receptor 1 - DAM1770302 DAM1770302
      Anti-GABAA Receptor 1 - DAM1791343 DAM1791343

      References

      Reference overviewApplicationSpeciesPub Med ID
      Cryoloading: introducing large molecules into live synaptosomes.
      Nath, AR; Chen, RH; Stanley, EF
      Frontiers in cellular neuroscience  8  4  2014

      Show Abstract
      24478628 24478628
      Synaptic function of nicastrin in hippocampal neurons.
      Lee, SH; Sharma, M; Südhof, TC; Shen, J
      Proceedings of the National Academy of Sciences of the United States of America  111  8973-8  2014

      Show Abstract
      Western BlottingMouse24889619 24889619
      The effect of STAT3 inhibition on status epilepticus and subsequent spontaneous seizures in the pilocarpine model of acquired epilepsy.
      Grabenstatter, HL; Del Angel, YC; Carlsen, J; Wempe, MF; White, AM; Cogswell, M; Russek, SJ; Brooks-Kayal, AR
      Neurobiology of disease  62  73-85  2014

      Show Abstract
      Western Blotting24051278 24051278
      Expression of GABAA α2-, β1- and ε-receptors are altered significantly in the lateral cerebellum of subjects with schizophrenia, major depression and bipolar disorder.
      Fatemi, SH; Folsom, TD; Rooney, RJ; Thuras, PD
      Translational psychiatry  3  e303  2013

      Show Abstract
      Western BlottingHuman24022508 24022508
      Altered cortical GABAA receptor composition, physiology, and endocytosis in a mouse model of a human genetic absence epilepsy syndrome.
      Zhou, C; Huang, Z; Ding, L; Deel, ME; Arain, FM; Murray, CR; Patel, RS; Flanagan, CD; Gallagher, MJ
      The Journal of biological chemistry  288  21458-72  2013

      Show Abstract
      23744069 23744069
      An autocrine γ-aminobutyric acid signaling system exists in pancreatic β-cell progenitors of fetal and postnatal mice.
      Feng, MM; Xiang, YY; Wang, S; Lu, WY
      International journal of physiology, pathophysiology and pharmacology  5  91-101  2013

      Show Abstract
      Immunohistochemistry23750307 23750307
      Baicalein reduces β-amyloid and promotes nonamyloidogenic amyloid precursor protein processing in an Alzheimer's disease transgenic mouse model.
      Zhang, SQ; Obregon, D; Ehrhart, J; Deng, J; Tian, J; Hou, H; Giunta, B; Sawmiller, D; Tan, J
      Journal of neuroscience research  91  1239-46  2013

      Show Abstract
      Western Blotting23686791 23686791
      Tracking the expression of excitatory and inhibitory neurotransmission-related proteins and neuroplasticity markers after noise induced hearing loss.
      Browne, CJ; Morley, JW; Parsons, CH
      PloS one  7  e33272  2012

      Show Abstract
      22428005 22428005
      Proteasome Inhibition Alleviates SNARE-Dependent Neurodegeneration.
      Sharma, Manu, et al.
      Sci Transl Med, 4: 147ra113 (2012)  2012

      Show Abstract
      22896677 22896677
      CSPα knockout causes neurodegeneration by impairing SNAP-25 function.
      Sharma, M; Burré, J; Bronk, P; Zhang, Y; Xu, W; Südhof, TC
      The EMBO journal  31  829-41  2012

      Show Abstract
      22187053 22187053