05-924 | Anti-GFI1 Antibody, clone 2.5D.17

05-924
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-924
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-GFI1 Antibody, clone 2.5D.17
      Alternate Names
      • Growth Factor Independent 1
      References
      Product Information
      FormatPurified
      Applications
      ApplicationAnti-GFI1 Antibody, clone 2.5D.17 is a Mouse Monoclonal Antibody for detection of GFI1 also known as Growth Factor Independent 1 & has been validated in IP, WB & IHC.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenGST-fusion protein corresponding to residues 1-250 of rat growth factor independence-1 (GFI1).
      Clone2.5D.17
      HostMouse
      SpecificityGFI1
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GFI1
      • ZNF163
      Non-Reactive Species
      • Mouse
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q99684 # May be a transcription factor involved in regulating the expression of genes active in the S phase during cell cycle progression in T-cells. May be involved in tumor progression (By similarity). Represses ELA2 transcription.
      SIZE: 422 amino acids; 45297 Da
      SUBUNIT: Interacts with U2AF1L4 (By similarity).
      SUBCELLULAR LOCATION: Nucleus.
      DOMAIN:SwissProt: Q99684 The Gly/Ala-rich region may indicate that Gfi-1 is a repressor of its target genes.
      DISEASE: SwissProt: Q99684 # Defects in GFI1 are a cause of autosomal dominant severe congenital neutropenia (SCN) [MIM:202700]. & Defects in GFI1 are a cause of dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA) [MIM:607847]. NI-CINA is a relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.
      SIMILARITY: Contains 6 C2H2-type zinc fingers.
      Molecular Weight55-65kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GFI1, clone 2.5D.17 - 30453 30453

      References

      Reference overviewApplicationPub Med ID
      Gfi1 expressed in bone marrow stromal cells is a novel osteoblast suppressor in patients with multiple myeloma bone disease.
      D'Souza, S; del Prete, D; Jin, S; Sun, Q; Huston, AJ; Kostov, FE; Sammut, B; Hong, CS; Anderson, JL; Patrene, KD; Yu, S; Velu, CS; Xiao, G; Grimes, HL; Roodman, GD; Galson, DL
      Blood  118  6871-80  2011

      Show Abstract
      22042697 22042697
      The growth factor independence-1 transcription factor: new functions and new insights
      Kazanjian, Avedis, et al
      Crit Rev Oncol Hematol, 59:85-97 (2006)  2006

      16716599 16716599
      Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells
      Doan, L. L., et al
      Nucleic Acids Res, 32:2508-19 (2004)  2004

      Immunoblotting (Western)15131254 15131254
      Growth factor independence-1 is expressed in primary human neuroendocrine lung carcinomas and mediates the differentiation of murine pulmonary neuroendocrine cells.
      Kazanjian, Avedis, et al.
      Cancer Res., 64: 6874-82 (2004)  2004

      Show Abstract
      Immunohistochemistry (Tissue)15466176 15466176

      FAQ

      QuestionAnswer
      I would like to use this monoclonal antibody on mouse tissue, are there any special procedures I should follow?To reduce the background interaction between the monoclonal antibody and the mouse tissue, you will need to perform antigen retrieval and use a special secondary antibody. For antigen retrieval, prepare a citrate buffer by mixing 19ml of 0.1M citric acid and 82ml of 0.1M sodium citrate to make 1000ml of buffer. Adjust the pH to 6.0 before each use. Add buffer to your slide. Using an 800 watt microwave oven with rotation, microwave for 7.5 min at high, 5 min at 50% (3 cycles), checking the slide at the end of each cycle to see if the solution needs to be replaced, adding water if needed. For the secondary antibody, use a biotinylated isotype specific secondary IGG (IGG-1) and a 3% donkey serum block. This will help reduce the background issues using mouse monoclonals.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies