07-1401 | Anti-GLUT-1 Antibody, CT

07-1401
50 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information
      Replacement Information07-1401 is a recommended replacement for AB1340

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IH(P), ICC, ELISARbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1401
      ReplacesAB1340
      DescriptionAnti-GLUT-1 Antibody, CT
      Alternate Names
      • Glucose Transporter type 1
      • Solute carrier family 2, facilitated glucose transporter, member 1
      Background InformationGLUTs (Glucose transporters) are a family of integral membrane proteins that facilitative glucose uptake by cells. They are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins, designated as GLUT 1 to 7, facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encode at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%), all in the molecular weight range of 40 to 60 kDa. GLUT-1 functions in constitutive or basal glucose uptake in adult tissues and is the predominant glucose transporter in embryonic and fetal tissues. It has two forms, a 55kDa and a 45 kDa. The 55kDa form of GLUT1 regulates import of glucose from blood to brain across the endothelial cells of the blood-brain barrier (BBB), whereas the 45 kDa form of GLUT1 predominantly regulates nonvascular glial glucose uptake. GLUT2 is a facilitative glucose transporter. This isoform likely mediates the bidirectional transfer of glucose across the plasma membrane of hepatocytes and is responsible for uptake of glucose by the beta cells; may comprise part of the glucose-sensing mechanism of the beta cell. May also participate with the Na(+)/glucose cotransporter in the transcellular transport of glucose in the small intestine and kidney. GLUT-4 is present in skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species. The expression of GLUT-4 is greatly influenced by insulin and other stimulators of the IP3 signaling pathway. Binding of insulin to the insulin receptor signals through PI3K and Akt to translocate GLUT-4 to the plasma membrane where it allows the uptake of glucose.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Jurkat Cell Lysate
      PresentationPurified rabbit polyclonal IgG in buffer containing 0.02 M Phosphate Buffer, 0.25 M NaCl, with 0.1% sodium azide.
      Applications
      ApplicationAnti-GLUT-1 Antibody, C-terminus is an antibody against C terminus of GLUT-1 that is validated for the detection of GLUT-1 in Western Blotting (WB), Immunohistochemistry - Paraffin (IHC-P), Immunocytochemistry (ICC) and ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      • ELISA
      Application NotesImmunohistochemisty (paraffin): GLUT-1 representative staining pattern/morphology on colorectal carcinoma. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. GLUT-1 immunoreactivity in erythrocytes.
      Note: can be use as internal control for IHC run.

      Immunocytochemistry: Recommended

      ELISA: Recommended

      Western Blot: A previous lot of this antibody was tested on multiple lysates using western blot analysis. See datasheet.
      Biological Information
      ImmunogenSynthetic peptide corresponding amino acids with the C-terminus of human GLUT-1 coupled to KLH (C-ELFHPLGADSQV).
      EpitopeC-Terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects GLUT-1 in human and mouse cells. It targets an epitope within 12 amino acids from the C-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman. Mouse. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a major glucose transporter in the mammalian blood-brain barrier. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq].

      Gene Symbol
      • GLUT
      • GLUT-1
      • GLUT1
      • MGC141895
      • MGC141896
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Melanosome. Note=Localizes primarily at the cell surface (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      TISSUE SPECIFICITY: Expressed at variable levels in many human tissues.
      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
      DISEASE: Defects in SLC2A1 are the cause of autosomal dominant GLUT1 deficiency syndrome [MIM:606777]. This disease causes a defect in glucose transport across the blood-brain barrier. It is characterized by infantile seizures, delayed development, and acquired microcephaly.
      SIMILARITY: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily [view classification].

      Molecular WeightThis antibody detects a major band ~54 kDa in Western blot analysis.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HUVEC.

      Western Blotting Analysis: 1 µg/mL of this antibody detected GLUT-1, CT in HUVEC.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GLUT-1 - 2142701 2142701
      Anti-GLUT-1 - 2373314 2373314
      Anti-GLUT-1 - 2430490 2430490
      Anti-GLUT-1 - 2459061 2459061
      Anti-GLUT-1 - 1975954 1975954
      Anti-GLUT-1 - 2026411 2026411
      Anti-GLUT-1 - 2043895 2043895
      Anti-GLUT-1 - 2283559 2283559
      Anti-GLUT-1 - 2489080 2489080
      Anti-GLUT-1 - 2500610 2500610

      References

      Reference overviewApplicationSpeciesPub Med ID
      Periostin secreted by glioblastoma stem cells recruits M2 tumour-associated macrophages and promotes malignant growth.
      Zhou, W; Ke, SQ; Huang, Z; Flavahan, W; Fang, X; Paul, J; Wu, L; Sloan, AE; McLendon, RE; Li, X; Rich, JN; Bao, S
      Nature cell biology  17  170-82  2015

      Show Abstract
      25580734 25580734
      Partial eNOS deficiency causes spontaneous thrombotic cerebral infarction, amyloid angiopathy and cognitive impairment.
      Tan, XL; Xue, YQ; Ma, T; Wang, X; Li, JJ; Lan, L; Malik, KU; McDonald, MP; Dopico, AM; Liao, FF
      Molecular neurodegeneration  10  24  2015

      Show Abstract
      26104027 26104027
      The role of Hypoxia-inducible factor-1 α , glucose transporter-1, (GLUT-1) and carbon anhydrase IX in endometrial cancer patients.
      Sadlecki, P; Bodnar, M; Grabiec, M; Marszalek, A; Walentowicz, P; Sokup, A; Zegarska, J; Walentowicz-Sadlecka, M
      BioMed research international  2014  616850  2014

      Show Abstract
      Immunohistochemistry (Paraffin)Human24745019 24745019
      A conditional mouse mutant in the tumor suppressor SdhD gene unveils a link between p21(WAF1/Cip1) induction and mitochondrial dysfunction.
      Millán-Uclés, A; Díaz-Castro, B; García-Flores, P; Báez, A; Pérez-Simón, JA; López-Barneo, J; Piruat, JI
      PloS one  9  e85528  2014

      Show Abstract
      Western Blotting24465590 24465590
      Hyperglycemia promotes K-Ras-induced lung tumorigenesis through BASCs amplification.
      Micucci, C; Orciari, S; Catalano, A
      PloS one  9  e105550  2014

      Show Abstract
      25144301 25144301
      Palmitoleic acid (n-7) increases white adipocytes GLUT4 content and glucose uptake in association with AMPK activation.
      Bolsoni-Lopes, A; Festuccia, WT; Chimin, P; Farias, TS; Torres-Leal, FL; Cruz, MM; Andrade, PB; Hirabara, SM; Lima, FB; Alonso-Vale, MI
      Lipids in health and disease  13  199  2014

      Show Abstract
      Western Blotting25528561 25528561
      GLUT1 protein expression correlates with unfavourable histologic category and high risk in patients with neuroblastic tumours.
      Ramani, Pramila, et al.
      Virchows Arch., (2013)  2013

      Show Abstract
      23307190 23307190
      Serotonin (5-HT) affects expression of liver metabolic enzymes and mammary gland glucose transporters during the transition from pregnancy to lactation.
      Laporta, J; Peters, TL; Merriman, KE; Vezina, CM; Hernandez, LL
      PloS one  8  e57847  2013

      Show Abstract
      23469086 23469086
      Impact of tumor vascularity on responsiveness to antiangiogenesis in a prostate cancer stem cell-derived tumor model.
      Zhang, K; Waxman, DJ
      Molecular cancer therapeutics  12  787-98  2013

      Show Abstract
      Immunohistochemistry23635653 23635653
      Fibronectin extra domain A (EDA) sustains CD133(+)/CD44(+) subpopulation of colorectal cancer cells.
      Ou, J; Deng, J; Wei, X; Xie, G; Zhou, R; Yu, L; Liang, H
      Stem cell research  11  820-33  2013

      Show Abstract
      23811539 23811539

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      07-1401-AF488 Anti-GLUT-1 Antibody, CT, Alexa Fluor® 488 Conjugate Show Pricing & Availability
      ABN991 Anti-phospho GLUT-1 Antibody (Ser226) Show Pricing & Availability
      CBL242 Anti-Glucose Transporter GLUT-1 Antibody, CT Show Pricing & Availability
      MABS132 Anti-GLUT1 (CT) Antibody, clone 5B12.3 Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies