05-372 | Anti-GRB2 Antibody, clone 3F2

05-372
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, Ht, M, RIHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-372
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-GRB2 Antibody, clone 3F2
      References
      Product Information
      FormatPurified
      PresentationPBS containing 50% glycerol
      Applications
      ApplicationThis Anti-GRB2 Antibody, clone 3F2 is validated for use in IH, WB for the detection of GRB2.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesNot recommended for IP.
      Biological Information
      ImmunogenRecombinant GRB2/Ash
      Cloneclone 3F2
      HostMouse
      SpecificityN-terminal SH3 region of GRB2
      IsotypeIgG1κ
      Species Reactivity
      • Bovine
      • Human
      • Hamster
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene binds the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the Sem5 gene of C.elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • GRB2
      • EGFRBP-GRB2
      • HT027
      • ASH
      • MST084
      • MSTP084
      • Grb3-3
      Purification MethodProtein A chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62993 # Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.
      SIZE: 217 amino acids; 25206 Da
      SUBUNIT: Associates with activated Tyr-phosphorylated EGF receptors and PDGF receptors via its SH2 domain. Also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, SHC and LNK; probably via the concerted action of both its SH2 and SH3 domains. It also seems to interact with RAS in the signaling pathway leading to DNA synthesis. Binds to and translocates the guanine nucleotide exchange factors SOS. Interacts with phosphorylated TOM1L1 and MET. Interacts with the phosphorylated C-terminus of SH2B2. Interacts with phosphorylated SIT1, LAX1, LAT, LAT2 and LIME1 upon TCR and/or BCR activation. Interacts with PTPNS1, REPS2 and the syntrophin SNTA1. Interacts with REPS1 and PIK3C2B via its SH3 domains (By similarity). Interacts with HCV NS5A via its SH3 domains. Interacts with CBL and CBLB. Interacts with SHB, INPP5D/SHIP1, SKAP1 and SKAP2.
      DOMAIN: SwissProt: P62993 The SH3 domains mediate interaction with SHB.
      SIMILARITY: Belongs to the GRB2/sem-5/DRK family. & Contains 1 SH2 domain. & Contains 2 SH3 domains.
      Molecular Weight23-25kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Availability by Geography
      • This product is not available for sale in Japan.
      Quality Assuranceroutinely evaluated in immunoblot on RIPA lysates of non-stimulated human A431 carcinoma cells, RIPA lysates of mouse 3T3/A31 fibroblasts, rat L6 myoblasts, Chinese hamster ovary cells or bovine brain extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GRB2, clone 3F2 (mouse monoclonal IgG1K) 2935924
      Anti-GRB2, clone 3F2 (mouse monoclonal IgG1k) 2960526
      Anti-GRB2, clone 3F2 (mouse monoclonal IgG1k) - 1970691 1970691
      Anti-GRB2, clone 3F2 (mouse monoclonal IgG1k) - 2007676 2007676
      Anti-GRB2, clone 3F2 (mouse monoclonal IgG1k) - 2051322 2051322
      Anti-GRB2, clone 3F2 (mouse monoclonal IgG1k) - 2075083 2075083
      Anti-GRB2, clone 3F2 (mouse monoclonal IgG1k) - 2325023 2325023
      Anti-GRB2, clone 3F2 (mouse monoclonal IgG1k) - NG1916493 NG1916493
      Anti-GRB2, clone 3F2 (mouse monoclonal IgG1κ) 2874331
      Anti-GRB2, clone 3F2 - 15659 15659

      References

      Reference overviewApplicationPub Med ID
      A novel protein kinase C alpha-dependent signal to ERK1/2 activated by alphaVbeta3 integrin in osteoclasts and in Chinese hamster ovary (CHO) cells.
      Rucci, N; DiGiacinto, C; Orrù, L; Millimaggi, D; Baron, R; Teti, A
      Journal of cell science  118  3263-75  2005

      Show Abstract
      16014375 16014375
      Proteome analysis reveals caspase activation in hyporesponsive CD4 T lymphocytes induced in vivo by the oral administration of antigen
      Kaji, T., et al
      J Biol Chem, 278:27836-43 (2003)  2003

      Immunoblotting (Western)12736267 12736267
      The multisubstrate docking site of the MET receptor is dispensable for MET-mediated RAS signaling and cell scattering
      Tulasne, D., et al
      Mol Biol Cell, 10:551-65 (1999)  1999

      Immunoblotting (Western)10069803 10069803
      The calcitonin receptor stimulates Shc tyrosine phosphorylation and Erk1/2 activation. Involvement of Gi, protein kinase C, and calcium
      Chen, Y., et al
      J Biol Chem, 273:19809-16 (1998)  1998

      Immunoprecipitation9677414 9677414
      T cell receptor-induced phosphorylation of Sos requires activity of CD45, Lck, and protein kinase C, but not ERK.
      Zhao, H, et al.
      J. Biol. Chem., 272: 21625-34 (1997)  1997

      Show Abstract
      Phosphatase Assay9261185 9261185
      c-Cbl is transiently tyrosine-phosphorylated, ubiquitinated, and membrane-targeted following CSF-1 stimulation of macrophages
      Wang, Y., et al
      J Biol Chem, 271:17-20 (1996)  1996

      Immunoblotting (Western)8550554 8550554
      Protein-tyrosine phosphatase 1D modulates its own state of tyrosine phosphorylation
      Stein-Gerlach, M., et al
      J Biol Chem, 270:24635-7 (1995)  1995

      Immunoblotting (Western)7559570 7559570
      The SH2 and SH3 domains of mammalian Grb2 couple the EGF receptor to the Ras activator mSos1.
      Rozakis-Adcock, M, et al.
      Nature, 363: 83-5 (1993)  1993

      Show Abstract
      8479540 8479540
      The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling.
      Lowenstein, E J, et al.
      Cell, 70: 431-42 (1992)  1992

      Show Abstract
      1322798 1322798
      Cloning of ASH, a ubiquitous protein composed of one Src homology region (SH) 2 and two SH3 domains, from human and rat cDNA libraries.
      Matuoka, K, et al.
      Proc. Natl. Acad. Sci. U.S.A., 89: 9015-9 (1992)  1992

      Show Abstract
      1384039 1384039

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies