05-466 | Anti-GRK 4-6 Antibody, clone A16/17

05-466
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-466
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-GRK 4-6 Antibody, clone A16/17
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15 NaCl with 0.05% sodium azide
      Applications
      ApplicationAnti-GRK 4-6 Antibody, clone A16/17 detects level of GRK 4-6 & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 463-590 of bovine GRK 5
      Cloneclone A16/17
      HostMouse
      SpecificityGRK 4, GRK 5 and GRK 6
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension.
      Gene Symbol
      • GRK4
      • GRK4a
      • IT11
      • GPRK4
      • GPRK2L
      • ITI1
      Purification MethodAmmonium Sulfate Precipitation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P32298 # Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin.
      SIZE: 578 amino acids; 66583 Da
      TISSUE SPECIFICITY: Testis, and in a lower extent in other tissues including brain cortex and striatum.
      SIMILARITY: SwissProt: P32298 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain. & Contains 1 RGS domain.
      Molecular Weight65-70kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on murine 3T3/A31 RIPA lysate or human K562 cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GRK 4-6, clone A16/17 2477910
      Anti-GRK 4-6, clone A16/17 (mouse monoclonal IgG1κ) - 2391101 2391101
      Anti-GRK 4-6, clone A16/17 (mouse monoclonal IgG1k) 2897068
      Anti-GRK 4-6, clone A16/17 (mouse monoclonal IgG1k) - 2017305 2017305
      Anti-GRK 4-6, clone A16/17 - 16391 16391
      Anti-GRK 4-6, clone A16/17 - 20081215 20081215
      Anti-GRK 4-6, clone A16/17 - 2068170 2068170
      Anti-GRK 4-6, clone A16/17 - 20912 20912
      Anti-GRK 4-6, clone A16/17 - 26447 26447
      Anti-GRK 4-6, clone A16/17 - DAM1614906 DAM1614906

      References

      Reference overviewApplicationSpeciesPub Med ID
      Nuclear translocation of cardiac G protein-Coupled Receptor kinase 5 downstream of select Gq-activating hypertrophic ligands is a calmodulin-dependent process.
      Gold, JI; Martini, JS; Hullmann, J; Gao, E; Chuprun, JK; Lee, L; Tilley, DG; Rabinowitz, JE; Bossuyt, J; Bers, DM; Koch, WJ
      PloS one  8  e57324  2013

      Show Abstract
      Western Blotting23472081 23472081
      Toll-like receptors differentially regulate GPCR kinases and arrestins in primary macrophages.
      Loniewski, Katie, et al.
      Mol. Immunol., 45: 2312-22 (2008)  2008

      Show Abstract
      Mouse18180038 18180038
      Phosphorylation and desensitization of human endothelin A and B receptors. Evidence for G protein-coupled receptor kinase specificity.
      Freedman, N J, et al.
      J. Biol. Chem., 272: 17734-43 (1997)  1997

      Show Abstract
      9211925 9211925
      Involvement of G protein-coupled receptor kinase 5 in homologous desensitization of the thyrotropin receptor.
      Nagayama, Y, et al.
      J. Biol. Chem., 271: 10143-8 (1996)  1996

      Show Abstract
      8626574 8626574
      Monoclonal antibodies reveal receptor specificity among G-protein-coupled receptor kinases.
      Oppermann, M, et al.
      Proc. Natl. Acad. Sci. U.S.A., 93: 7649-54 (1996)  1996

      Show Abstract
      8755530 8755530
      Phosphatidylinositol 4,5-bisphosphate (PIP2)-enhanced G protein-coupled receptor kinase (GRK) activity. Location, structure, and regulation of the PIP2 binding site distinguishes the GRK subfamilies.
      Pitcher, J A, et al.
      J. Biol. Chem., 271: 24907-13 (1996)  1996

      Show Abstract
      8798768 8798768

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies