MABT829 | Anti-GRP94 Antibody, clone 3C4

MABT829
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Rb, GtWB, IF, IHC, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT829
      DescriptionAnti-GRP94 Antibody, clone 3C4
      Alternate Names
      • Endoplasmin
      • 94 kDa glucose-regulated protein
      • GRP-94
      • Heat shock protein 90 kDa beta member 1
      • GRP94
      Background InformationEndoplasmin (UniProt P14625; also known as 94 kDa glucose-regulated protein, Endothelial cell (HBMEC) glycoprotein, Epididymis luminal protein 35, Epididymis secretory sperm binding protein Li 125m, gp96 homolog, GRP-94, Heat shock protein 90 kDa beta member 1, Stress-inducible tumor rejection antigen gp96, Tumor rejection antigen 1) is encoded by the HSP90B1 (also known as ECGP, GP96, GRP94, HEL35; HEL-S-125m, TRA1) gene (Gene ID 7184) in human. The endoplasmic reticulum (ER) chaperone Grp94 mediates the cell surface export of molecules involved in the native immune response, mesoderm induction, muscle development, cytoprotection against oxidative stress, and calcium homeostasis. Src family tyrosine kinase Fyn-catalyzed Grp94 phosphorylation is reported to be essential for Grp94 export from ER to the Golgi apparatus in the early phase of myoblast differentiation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-GRP94 Antibody, clone 3C4 is an antibody against GRP94 for use in Western Blotting, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry
      • Immunoprecipitation
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected GRP94 in 10 µg of mouse liver and human heart tissue lysate.
      Immunofluorescence Analysis: Representative lots detected GRP94 immunoreactivity in rabbit and goat ventricular myocardium sections (Vitadello, M., et al. (1998). Biochem J. 332 ( Pt 2):351-359; Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206)
      Immunohistochemistry Analysis: A representative lot detected GRP94 immunoreactivity in fibrillating goat atria (Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206)
      Western Blotting Analysis: Representative lots detected GRP94 in atrial myocytes from vairous species, including murine (Milano, G., et al. (2013). PLoS One. 8(10):e76659), goat and human (Vitadello, M., et al. (2001). Circulation. 103(17):2201-2206), and rabbit (Vitadello, M., et al. (1998). Biochem J. 332 ( Pt 2):351-359).
      Immunoprecipitation Analysis: A representative lot co-immunoprecipitated nNOS with GRP94 from chemically cross-linked C2C12 murine myoblasts in either proliferating or differentiating cultures (Vitadello, M., et al. (2014). Antioxid Redox Signal. 20(16):2479-2476).
      Immunocytochemistry Analysis: A representative lot detected GRP94 immunoreactivity in murine C2C12 skeletal myoblasts as well as primary skeletal myocytes from new born mice (Gorza, L., and Vitadello, M. (2000). FASEB J. 14(3):461-475).
      Biological Information
      ImmunogenHis-tagged recombinant protein corresponding to the N-terminal of rabbit GRP94.
      EpitopeN-terminal half
      Clone3C4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Human
      • Rabbit
      • Goat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HSP90B1
      • GRP94
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~94/110 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in murine myoblast C2C12 cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected GRP94 in 10 µg of murine myoblast C2C12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GRP94, clone 3C4 -Q2589908 Q2589908

      References

      Reference overviewPub Med ID
      The stress protein/chaperone Grp94 counteracts muscle disuse atrophy by stabilizing subsarcolemmal neuronal nitric oxide synthase.
      Vitadello, M; Gherardini, J; Gorza, L
      Antioxidants & redox signaling  20  2479-96  2014

      Show Abstract
      24093939 24093939
      Impact of the phosphatidylinositide 3-kinase signaling pathway on the cardioprotection induced by intermittent hypoxia.
      Milano, G; Abruzzo, PM; Bolotta, A; Marini, M; Terraneo, L; Ravara, B; Gorza, L; Vitadello, M; Burattini, S; Curzi, D; Falcieri, E; von Segesser, LK; Samaja, M
      PloS one  8  e76659  2013

      Show Abstract
      24124584 24124584
      Increased myocardial GRP94 amounts during sustained atrial fibrillation: a protective response?
      Vitadello, M; Ausma, J; Borgers, M; Gambino, A; Casarotto, DC; Gorza, L
      Circulation  103  2201-6  2001

      Show Abstract
      11331263 11331263
      Reduced amount of the glucose-regulated protein GRP94 in skeletal myoblasts results in loss of fusion competence.
      Gorza, L; Vitadello, M
      FASEB journal : official publication of the Federation of American Societies for Experimental Biology  14  461-75  2000

      Show Abstract
      10698961 10698961
      Rabbit cardiac and skeletal myocytes differ in constitutive and inducible expression of the glucose-regulated protein GRP94.
      Vitadello, M; Colpo, P; Gorza, L
      The Biochemical journal  332 ( Pt 2)  351-9  1998

      Show Abstract
      9601063 9601063

      Technical Info

      Title
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      White Paper: Further considerations of antibody validation and usage.