04-903 | Anti-GSK3α/β Antibody, rabbit monoclonal

04-903
100 µl  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IPRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-903
      Replaces05-903
      DescriptionAnti-GSK3α/β Antibody, rabbit monoclonal
      Alternate Names
      • GSK-3 alpha
      • glycogen synthase kinase 3 alpha
      Background InformationGlycogen Synthase Kinase 3 differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • HEK293 cell lysate
      PresentationCultured supernantant containing 0.05% sodium azide.
      Applications
      ApplicationThis Anti-GSK3α/β Antibody is validated for use in WB, IP for the detection of GSK3α/β.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation: 2-8 μL of a previous lot of antibody immunoprecipitated GSK3 alpha and beta from 100 μg of HEK293 RIPA lysate.
      NOTE: This antibody also successfully immunoprecipitated GSK3 alpha and beta using the Catch and Release® v2.0 Reversible Immunoprecipitation System (Catalog # 17-500).
      Biological Information
      ImmunogenN-terminal 6His-tagged recombinant full length human GSK3β.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityGSK3 alpha and beta.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman and mouse.
      Predicted to cross-react with rat based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase,
      homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of
      several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription
      factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)
      Gene Symbol
      • DKFZp686D0638
      • EC 2.7.11.26
      UniProt Number
      UniProt SummaryFUNCTION:Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN By similarity.
      CATALYTIC ACTIVITY: ATP + [tau protein] = ADP + [tau protein] phosphate.
      SUBUNIT STRUCTURE:Monomer By similarity.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
      Contains 1 protein kinase domain.

      Molecular Weight~46 kDa (beta) and 51 kDa (alpha)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on lysates from HEK293 cells.
      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GSK3 alpha and GSK3 beta in RIPA lysates from A431, HEK293, Jurkat, HeLa NE or 3T3/A31 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information
      Specifications