06-236 | Anti-Giα1/2 Antibody

06-236
50 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      B, H, M, RICC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-236
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Giα1/2 Antibody
      References
      Product Information
      FormatPurified
      Control
      • Widely expressed
      PresentationA diluted rabbit serum in PBS (50 mM KHPO4, 140 mM NaCl). Frozen solution
      Applications
      ApplicationDetect Giα1/2 using this Anti-Giα1/2 Antibody validated for use in IC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      Immunogen10 residue synthetic peptide (KNNLKDCGLF) corresponding to the c-terminus of Gia1 and Gia2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes Giα1 and Giα2.
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGuanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM]
      Gene Symbol
      • GNAI1
      • Gi
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63096 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli.
      SIZE: 354 amino acids; 40361 Da
      SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity).
      SIMILARITY: SwissProt: P63096 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.
      Molecular Weight40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on Rat Brain Microsomal Preparation or solubilized plasma membranes from mouse 3T3/A31 fibroblasts
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Certificates of Analysis

      TitleLot Number
      Anti-Gi 1-2 (rabbit polyclonal IgG) - JBC1926732 JBC1926732
      Anti-Gi#945; 1-2 - DAM1821102 DAM1821102
      Anti-Gi(alpha) 1-2 (rabbit polyclonal IgG) - 2211902 2211902
      Anti-Gia 1-2 (rabbit polyclonal IgG) - 1964519 1964519
      Anti-Gia 1-2 (rabbit polyclonal IgG) - NG1936155 NG1936155
      Anti-Gia 1-2 - DAM1723985 DAM1723985
      Anti-Gialpha;1/2 - 0703054340 0703054340
      Anti-Gialpha;1/2 - 15343 15343
      Anti-Gialpha;1/2 - 21083 21083
      Anti-Gialpha;1/2 - 23548 23548

      References

      Reference overviewApplicationPub Med ID
      Compartmentalization of signaling-competent epidermal growth factor receptors in endosomes.
      Balbis, A; Parmar, A; Wang, Y; Baquiran, G; Posner, BI
      Endocrinology  148  2944-54  2007

      Show Abstract
      17363458 17363458
      Subcellular localization of LGN during mitosis: evidence for its cortical localization in mitotic cell culture systems and its requirement for normal cell cycle progression.
      Kaushik, R; Yu, F; Chia, W; Yang, X; Bahri, S
      Molecular biology of the cell  14  3144-55  2003

      Show Abstract Full Text Article
      12925752 12925752
      A role for tyrosine phosphorylation in the regulation and sensitization of adenylate cyclase by melatonin
      Barrett, P., et al
      Faseb J, 14:1619-28 (2000)  2000

      Immunoblotting (Western)10928997 10928997
      Disulfide bonds in the extracellular calcium-polyvalent cation-sensing receptor correlate with dimer formation and its response to divalent cations in vitro
      Ward, D. T., et al
      J Biol Chem, 273:14476-83 (1998)  1998

      Immunoblotting (Western)9603961 9603961
      Apical extracellular calcium/polyvalent cation-sensing receptor regulates vasopressin-elicited water permeability in rat kidney inner medullary collecting duct
      Sands, J. M., et al
      J Clin Invest, 99:1399-405 (1997)  1997

      Immunoblotting (Western)9077550 9077550
      Guanine nucleotide binding regulatory proteins and adenylate cyclase in livers of streptozotocin- and BB/Wor-diabetic rats. Immunodetection of Gs and Gi with antisera prepared against synthetic peptides.
      Lynch, C J, et al.
      J. Clin. Invest., 83: 2050-62 (1989)  1989

      Show Abstract
      2498395 2498395

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies