04-855 | Anti-GluR1 Antibody, clone C3T, rabbit monoclonal

04-855
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, ChpIHC, IP, WBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-855
      Replaces05-855
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-GluR1 Antibody, clone C3T, rabbit monoclonal
      Background InformationL glutamate is the major excitatory neurotransmitter in the central nervous system. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors that have been divided into 3 groups. Group I includes GRM1 and these receptors have been shown to activate phospholipase C. GRM1 expression has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • Routinely evaluated by immunoblot on rat brain microsomal preparation (12-144)
      PresentationCultured supernantant in 0.05% sodium azide
      Applications
      ApplicationAnti-GluR1 Antibody, clone C3T detects level of GluR1 & has been published & validated for use in IH, IP & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation: 2 μL of a previous lot immunoprecipitated GluR1 from rat brain microsomal preparation.

      Immunohistochemistry: A previous lot of this antibody has been shown to detect GluR1 in human, mouse and rat tissues.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 858-868 (..TLPRNSGAGAS..) of human GluR1 (amino acids 840-850 of mature human GluR1)
      CloneC3T
      HostRabbit
      SpecificityRecognizes GluR1, Mr 106 kDa.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Chimpanzee
      Species Reactivity NoteHuman. Mouse. Rat. Predicted to cross-react with Chimpanzee based pm 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGlutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • GRIA1
      • GluR-A
      • GluR-1
      • GLUR1
      • GluR-K1
      • GLURA
      • HBGR1
      • GLUH1
      • MGC133252
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P42261 # Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist.
      SIZE: 906 amino acids; 101506 Da
      SUBUNIT: Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with DLG1 via its C-terminus. Interacts with CACNG2 and RASGRF2 (By similarity).
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Interaction with CACNG2 promotes cell surface expression (By similarity).
      TISSUE SPECIFICITY: Widely expressed in brain.
      PTM: Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity).
      SIMILARITY: SwissProt: P42261 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
      MISCELLANEOUS: The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.
      Molecular Weight106 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western blotting of rat brain microsomal preparation.

      Western Blotting Analysis (WB): A 1:5,000-1:10,000 dilution of this lot detected GluR1 in rat brain microsomal preparation (Catalog # 12-144).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-GluR1, clone C3T 2462885
      Anti-GluR1, clone C3T - 2007357 2007357
      Anti-GluR1, clone C3T - 2146013 2146013
      Anti-GluR1, clone C3T - 2452486 2452486
      Anti-GluR1, clone C3T - NG1845570 NG1845570
      Anti-GluR1, clone C3T - 2026873 2026873
      Anti-GluR1, clone C3T - 2294368 2294368
      Anti-GluR1, clone C3T - DAM1532834 DAM1532834
      Anti-GluR1, clone C3T - JBC1771111 JBC1771111
      Anti-GluR1, clone C3T - JBC1771111 JBC1771111

      References

      Reference overviewApplicationPub Med ID
      Estrous Cycle-Dependent Phasic Changes in the Stoichiometry of Hippocampal Synaptic AMPA Receptors in Rats.
      Tada, H; Koide, M; Ara, W; Shibata, Y; Funabashi, T; Suyama, K; Goto, T; Takahashi, T
      PloS one  10  e0131359  2015

      Show Abstract
      26121335 26121335
      Synaptic dysregulation in a human iPS cell model of mental disorders.
      Wen, Z; Nguyen, HN; Guo, Z; Lalli, MA; Wang, X; Su, Y; Kim, NS; Yoon, KJ; Shin, J; Zhang, C; Makri, G; Nauen, D; Yu, H; Guzman, E; Chiang, CH; Yoritomo, N; Kaibuchi, K; Zou, J; Christian, KM; Cheng, L; Ross, CA; Margolis, RL; Chen, G; Kosik, KS; Song, H; Ming, GL
      Nature  2014

      Show Abstract
      25132547 25132547
      STAT1 regulates the homeostatic component of visual cortical plasticity via an AMPA receptor-mediated mechanism.
      Nagakura, I; Van Wart, A; Petravicz, J; Tropea, D; Sur, M
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  10256-63  2014

      Show Abstract
      Western Blotting25080587 25080587
      Gonadectomy differentially regulates steroid receptor coactivator-1 and synaptic proteins in the hippocampus of adult female and male C57BL/6 mice.
      Chen Bian,Kongjiang Zhu,Li Yang,Sen Lin,Shurong Li,Bingyin Su,Jiqiang Zhang
      Synapse (New York, N.Y.)  66  2012

      Show Abstract
      22623226 22623226
      Ethanol-mediated facilitation of AMPA receptor function in the dorsomedial striatum: implications for alcohol drinking behavior.
      Wang, J; Ben Hamida, S; Darcq, E; Zhu, W; Gibb, SL; Lanfranco, MF; Carnicella, S; Ron, D
      The Journal of neuroscience : the official journal of the Society for Neuroscience  32  15124-32  2012

      Show Abstract
      23100433 23100433
      Phosphorylation of the alpha-amino-3-hydroxy-5-methylisoxazole4-propionic acid receptor GluR1 subunit by calcium/calmodulin-dependent kinase II.
      Mammen, A L, et al.
      J. Biol. Chem., 272: 32528-33 (1997)  1997

      Show Abstract
      9405465 9405465
      The striatal mosaic in primates: striosomes and matrix are differentially enriched in ionotropic glutamate receptor subunits.
      Martin, L J, et al.
      J. Neurosci., 13: 782-92 (1993)  1993

      7678861 7678861
      The distribution of glutamate receptors in cultured rat hippocampal neurons: postsynaptic clustering of AMPA-selective subunits.
      Craig, A M, et al.
      Neuron, 10: 1055-68 (1993)  1993

      Show Abstract
      7686378 7686378
      Cellular localizations of AMPA glutamate receptors within the basal forebrain magnocellular complex of rat and monkey.
      Martin, L J, et al.
      J. Neurosci., 13: 2249-63 (1993)  1993

      Show Abstract
      8386757 8386757

      Brochure

      Title
      Pathways and Biomarkers of Glutamatergic Synapse Flyer

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies