04-902 | Anti-HA Tag Antibody, clone DW2, rabbit monoclonal

04-902
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtWBRbCulture SupernatantMonoclonal Antibody
      Description
      Catalogue Number04-902
      Replaces05-902
      DescriptionAnti-HA Tag Antibody, clone DW2, rabbit monoclonal
      Background InformationHuman influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule that corresponds to amino acids 98-106. It has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
      References
      Product Information
      FormatCulture Supernatant
      Control
      • HA-tagged WNT-1 protein.
      PresentationCultured supernatant in 0.05% sodium azide.
      Applications
      ApplicationAnti-HA Tag Antibody, clone DW2 is an antibody against HA Tag for use in WB.
      Key Applications
      • Western Blotting
      Application NotesWestern Blot Analysis: A 1:1,000-1:2,000 dilution of this lot detected HA-tagged WNT-1 protein expressed in HEK293 cells.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide containing the sequence …CYPYDVPDY… derived from the influenza virus hemaglutinin (HA) protein.

      CloneDW2
      HostRabbit
      SpecificityRecognizes recombinant proteins containing the HA epitope tag. The apparent molecular weight is related to the tagged protein.

      IsotypeIgG
      Species Reactivity
      • Vertebrates
      Species Reactivity NoteMost common vertebrate species tested. Reactivity with other species has not been confirmed.
      Antibody TypeMonoclonal Antibody
      Molecular WeightVarious
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceWestern Blot Analysis
      Lysates from non-transfected (lane 1) and HAtagged WNT-1 transfected (lane 2) HEK293 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HA-Tag, clone DW2 (1:1,000 dilution).
      Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

      Arrow indicates the HA-tagged protein (~49 kDa). The apparent molecular weight is related to the tagged protein.

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of shipment. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications