06-1294 | Anti-HCE Antibody

100 µl  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBShUnpurifiedPolyclonal Antibody
      Catalogue Number06-1294
      DescriptionAnti-HCE Antibody
      Alternate Names
      • RNA guanylyltransferase and 5'-phosphatase
      Background InformationThe HCE protein, also known as RNA guanylyltransferase and 5'-phosphatase is a bifunctional enzyme. In cells this protein functions as a mRNA-capping enzyme with mRNA guanylyltransferase activity in the C-terminal region and RNA 5'-triphosphatase activity in the N-terminal region. This protein catalyzes the first two steps of cap formation: removal of the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and transfer of the gmp moiety of GTP to the 5'-diphosphate terminus.
      Product Information
      • Human HCE protein
      PresentationUnpurified sheep polyclonal in serum with 0.05% sodium azide.
      ApplicationAnti-HCE Antibody is a Sheep Polyclonal Antibody for detection of HCE also known as RNA guanylyltransferase & 5'-phosphatase & has been validated in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes HCE.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CAP1A
      • DKFZp686J2031
      • HCAP1
      • HCE
      • HCE1
      • OTTHUMP00000016841
      • hCAP
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.
      CATALYTIC ACTIVITY: A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
      GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.
      SUBUNIT STRUCTURE: Interacts with SUPT5H and RNMT. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Nucleus By similarity.
      TISSUE SPECIFICITY: Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle.
      MISCELLANEOUS: Isoform 2 to isoform 4 lack mRNA 5'-guanylyltransferase activity due to disruptions of the GTase domain.
      SEQUENCE SIMILARITIES: In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.
      In the C-terminal section; belongs to the eukaryotic GTase family.
      Molecular Weight~68 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human HCE protein.
      Western Blot Analysis: A 1:1000 dilution of this antibody detected HCE on 10 µg of human HCE protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µl
      Transport Information
      Supplemental Information

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      Life Science Research > Antibodies and Assays > Primary Antibodies