05-690 | Anti-HP1γ Antibody, clone 42s2

05-690
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIHC, WB, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-690
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HP1γ Antibody, clone 42s2
      Alternate Names
      • HP1 gamma
      • HP1 gamma homolog
      • Heterochromatin protein 1 homolog gamma
      • Modifier 2 protein
      • chromobox homolog 3
      • chromobox homolog 3 (Drosophila HP1 gamma)
      • chromobox homolog 3 (HP1 gamma homolog
      • Drosophila)
      • heterochromatin protein HP1 gamma
      • heterochromatin-like protein 1
      Background InformationChromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication.
      Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated
      References
      Product Information
      FormatPurified
      Control
      • HeLa acid extracts, HEK 293 nuclear extracts.
      PresentationPurified mouse monoclonal IgG1 in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175M NaCl, 0.07% sodium azide, and 30% glycerol. Liquid at -20ºC.
      Applications
      ApplicationUse Anti-HP1γ Antibody, clone 42s2 (Mouse Monoclonal Antibody) validated in ChIP, IHC, WB to detect HP1γ also known as Heterochromatin protein 1 homolog gamma.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunohistochemistry:
      This antibody has been reported by an independent laboratory to detect HP1γ positive cells in breast tissue.

      Immunoprecipitation/Chromatin Immunoprecipitation:
      This antibody has been reported by an independent laboratory to immunoprecipitate HP1γ from nuclear extracts and formalin-cross-linked chromatin.
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 17-173 of human HP1γ protein. Clone 42s2.
      Clone42s2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes HP1γ protein, Mr ~25 kDa.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteReacts with human, mouse. Reactivity with other species has not been determined.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAt the nuclear envelope, the nuclear lamina and heterochromatin are adjacent to the inner nuclear membrane. The protein encoded by this gene binds DNA and is a component of heterochromatin. This protein also can bind lamin B receptor, an integral membrane protein found in the inner nuclear membrane. The dual binding functions of the encoded protein may explain the association of heterochromatin with the inner nuclear membrane. Two transcript variants encoding the same protein but differing in the 5' UTR, have been found for this gene.

      Transcript Variant: This variant (1) contains an alternate 5' exon but encodes the same protein as transcript variant 2.
      Gene Symbol
      • CBX3
      • HECH
      • HP1-GAMMA
      • HP1Hs-gamma
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13185 # Seems to be involved in transcriptional silencing in heterochromatin-like complexes. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. May contribute to the association of the heterochromatin with the inner nuclear membrane through its interaction with lamin B receptor (LBR). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
      SIZE: 183 amino acids; 20811 Da
      SUBUNIT: Binds directly to CHAF1A. Interacts with histone H3 methylated at 'Lys-9'. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with LBR, INCENP, TRIM28/TIF1B, SUV420H1, SUV420H2 and SP100. Interacts with TIF1A (By similarity). Interacts with MIS12 and C20orf127. Can interact directly with CBX5 via the chromoshadow domain.
      SUBCELLULAR LOCATION: Nucleus (Potential). Note=Associates with euchromatin and is largely excluded from constitutive heterochromatin. May be associated with microtubules and mitotic poles during mitosis (Potential).
      PTM: Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.
      SIMILARITY: SwissProt: Q13185 ## Contains 2 chromo domains.
      Molecular Weight25 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot in acid-extracted HeLa cell lysates (Catalog #13-112).

      Western Blot Analysis:
      0.01-0.1 µg/mL of this lot detected HP1γ protein in acid-extracted HeLa cell lysates (Catalog # 13-112).
      Sales RestrictionsDue to license agreement restrictions, this product cannot be purchased for resale.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HP1#947;, clone 42s2 - 2154181 2154181
      Anti-HP1, clone 42s2 - 2262769 2262769
      Anti-HP1, clone 42s2 - JBC1939926 JBC1939926
      Anti-HP1g, clone 42s2 - DAM1501782 DAM1501782
      Anti-HP1gamma;, clone 42s2 - 0608038477 0608038477
      Anti-HP1gamma;, clone 42s2 - 0703054298 0703054298
      Anti-HP1gamma;, clone 42s2 - 24258 24258
      Anti-HP1gamma;, clone 42s2 - JBC1361848 JBC1361848
      Anti-HP1γ, clone 42s2 - 2392295 2392295
      Anti-HP1γ, clone 42s2 -2557581 2557581

      References

      Reference overviewApplicationSpeciesPub Med ID
      HP1α mediates defective heterochromatin repair and accelerates senescence in Zmpste24-deficient cells.
      Liu, J; Yin, X; Liu, B; Zheng, H; Zhou, G; Gong, L; Li, M; Li, X; Wang, Y; Hu, J; Krishnan, V; Zhou, Z; Wang, Z
      Cell cycle (Georgetown, Tex.)  13  1237-47  2014

      Show Abstract
      24584199 24584199
      DNA methylation analysis of the macrosatellite repeat associated with FSHD muscular dystrophy at single nucleotide level.
      Huichalaf, C; Micheloni, S; Ferri, G; Caccia, R; Gabellini, D
      PloS one  9  e115278  2014

      Show Abstract
      25545674 25545674
      Proteomic and genomic approaches reveal critical functions of H3K9 methylation and heterochromatin protein-1γ in reprogramming to pluripotency.
      Sridharan, R; Gonzales-Cope, M; Chronis, C; Bonora, G; McKee, R; Huang, C; Patel, S; Lopez, D; Mishra, N; Pellegrini, M; Carey, M; Garcia, BA; Plath, K
      Nature cell biology  15  872-82  2013

      Show Abstract
      23748610 23748610
      A NF-κB-dependent dual promoter-enhancer initiates the lipopolysaccharide-mediated transcriptional activation of the chicken lysozyme in macrophages.
      Witham, J; Ouboussad, L; Lefevre, PF
      PloS one  8  e59389  2013

      Show Abstract
      23533622 23533622
      Heterochromatin protein 1 gamma and IκB kinase alpha interdependence during tumour necrosis factor gene transcription elongation in activated macrophages.
      Thorne, JL; Ouboussad, L; Lefevre, PF
      Nucleic acids research  40  7676-89  2012

      Show Abstract
      Mouse22649058 22649058
      Autophagy-dependent senescence in response to DNA damage and chronic apoptotic stress.
      Singh, K; Matsuyama, S; Drazba, JA; Almasan, A
      Autophagy  8  236-51  2012

      Show Abstract
      Western Blotting22240589 22240589
      CBX3 regulates efficient RNA processing genome-wide.
      Smallwood, A; Hon, GC; Jin, F; Henry, RE; Espinosa, JM; Ren, B
      Genome research  22  1426-36  2012

      Show Abstract
      22684280 22684280
      Dynamics and memory of heterochromatin in living cells.
      Hathaway, NA; Bell, O; Hodges, C; Miller, EL; Neel, DS; Crabtree, GR
      Cell  149  1447-60  2012

      Show Abstract
      22704655 22704655
      Suppression and recovery of BRCA1-mediated transcription by HP1γ via modulation of promoter occupancy.
      Choi, JD; Park, MA; Lee, JS
      Nucleic acids research  40  11321-38  2012

      Show Abstract
      23074186 23074186
      Activities of ICP0 involved in the reversal of silencing of quiescent herpes simplex virus 1.
      Ferenczy, MW; Ranayhossaini, DJ; Deluca, NA
      Journal of virology  85  4993-5002  2011

      Show Abstract
      21411540 21411540

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies