05-689 | Anti-HP1α Antibody, clone15.19s2

05-689
200 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Click To Print This Page

      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, VrtICC, IHC, IP, WB, ChIPMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-689
      ReplacesMABE479
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-HP1α Antibody, clone15.19s2
      Alternate Names
      • Antigen p25
      • HP1 alpha
      • HP1Hs alpha
      • Heterochromatin protein 1 homolog alpha
      • chromobox homolog 5 (Drosophila HP1 alpha)
      • chromobox homolog 5 (HP1 alpha homolog, Drosophila)
      • heterochromatin protein 1-alpha
      Background InformationHeterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and each contains a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated on Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases and the p150 subunit of chromatin-assembly factor-1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination and sumoylation, suggesting multiple means of regulation.
      References
      Product Information
      FormatPurified
      Control
      • HeLa, NIH/3T3 and COS cell lysates.
      PresentationPurified mouse monoclonal IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% Sodium Azide before the addition of glycerol to 30%.
      Applications
      ApplicationAnti-HP1α Antibody, clone15.19s2 is a Mouse Monoclonal Antibody for detection of HP1α also known as HP1Hs alpha, Heterochromatin protein 1 homolog alpha & has been validated in ChIP, ICC, IHC, IP & WB.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunohistochemistry:
      A previous lot of this antibody has been reported by an independent laboratory to detect HP1a in NIH/3T3 nuclei (Ayyanathan, K., 2003).

      Immunoprecipitation/Chromatin Immunoprecipitation:
      A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate HP1α from nuclear extracts and formalin-cross-linked chromatin (Ayyanathan, K., 2003).
      Biological Information
      ImmunogenGST fusion protein corresponding to residues 2-191 of human HP1α protein.
      Clone15.19s2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes HP1α
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHeterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing.[supplied by OMIM]
      Gene Symbol
      • CBX5
      • HP1A
      • HP1Hs-alpha
      • HP1-ALPHA
      • HP1
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P45973 # Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Can interact with lamin B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
      SIZE: 191 amino acids; 22225 Da
      SUBUNIT: Interacts with SUV420H1 and SUV420H2 (By similarity). Interacts directly with ATRX, CHAF1A, LBR, NIPBL, SP100, STAM2 and TRIM28 via the chromoshadow domain. Can interact directly with CBX3 via the chromoshadow domain. Interacts with histone H3 methylated at 'Lys-9'. Interacts with MIS12 and C20orf127.
      SUBCELLULAR LOCATION: Nucleus. Centromere. Note=Component of centromeric and pericentromeric heterochromatin. Associates with chromosomes during mitosis. Associates specifically with chromatin during metaphase and anaphase.
      PTM: Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper- phosphorylated during mitosis.
      SIMILARITY: SwissProt: P45973 ## Contains 2 chromo domains.
      Molecular Weight29 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot in acid-extracted HeLa cell lysates.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected HP1α protein in acid-extracted HeLa cell lysates.
      Sales RestrictionsDue to license agreement restrictions, this product cannot be purchased for resale.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-HP1#945;, clone15.19s2 - 2153146 2153146
      Anti-HP1#945;, clone15.19s2 - 2021757 2021757
      Anti-HP1#945;, clone15.19s2 - DAM1770284 DAM1770284
      Anti-HP1#945;, clone15.19s2 - DAM1810805 DAM1810805
      Anti-HP1, clone15.19s2 - 1964540 1964540
      Anti-HP1, clone15.19s2 - 2043519 2043519
      Anti-HP1, clone15.19s2 - 2297222 2297222
      Anti-HP1, clone15.19s2 - DAM1412887 DAM1412887
      Anti-HP1, clone15.19s2 - DAM1650326 DAM1650326
      Anti-HP1, clone15.19s2 - DAM1695859 DAM1695859

      References

      Reference overviewApplicationSpeciesPub Med ID
      Epigenetic silencing of Oct4 by a complex containing SUV39H1 and Oct4 pseudogene lncRNA.
      Scarola, M; Comisso, E; Pascolo, R; Chiaradia, R; Maria Marion, R; Schneider, C; Blasco, MA; Schoeftner, S; Benetti, R
      Nature communications  6  7631  2015

      Show Abstract
      26158551 26158551
      Hierarchical clustering of breast cancer methylomes revealed differentially methylated and expressed breast cancer genes.
      Lin, IH; Chen, DT; Chang, YF; Lee, YL; Su, CH; Cheng, C; Tsai, YC; Ng, SC; Chen, HT; Lee, MC; Chen, HW; Suen, SH; Chen, YC; Liu, TT; Chang, CH; Hsu, MT
      PloS one  10  e0118453  2015

      Show Abstract
      25706888 25706888
      Dynamics of the two heterochromatin types during imprinted X chromosome inactivation in vole Microtus levis.
      Vaskova, EA; Dementyeva, EV; Shevchenko, AI; Pavlova, SV; Grigor'eva, EV; Zhelezova, AI; Vandeberg, JL; Zakian, SM
      PloS one  9  e88256  2014

      Show Abstract
      24505450 24505450
      Polycomb repressive complex 2 and H3K27me3 cooperate with H3K9 methylation to maintain heterochromatin protein 1α at chromatin.
      Boros, J; Arnoult, N; Stroobant, V; Collet, JF; Decottignies, A
      Molecular and cellular biology  34  3662-74  2014

      Show Abstract
      Western Blotting25047840 25047840
      A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli.
      Smith, CL; Matheson, TD; Trombly, DJ; Sun, X; Campeau, E; Han, X; Yates, JR; Kaufman, PD
      Molecular biology of the cell  25  2866-81  2014

      Show Abstract
      25057015 25057015
      HP1α mediates defective heterochromatin repair and accelerates senescence in Zmpste24-deficient cells.
      Liu, J; Yin, X; Liu, B; Zheng, H; Zhou, G; Gong, L; Li, M; Li, X; Wang, Y; Hu, J; Krishnan, V; Zhou, Z; Wang, Z
      Cell cycle (Georgetown, Tex.)  13  1237-47  2014

      Show Abstract
      24584199 24584199
      Developmentally regulated linker histone H1c promotes heterochromatin condensation and mediates structural integrity of rod photoreceptors in mouse retina.
      Popova, EY; Grigoryev, SA; Fan, Y; Skoultchi, AI; Zhang, SS; Barnstable, CJ
      The Journal of biological chemistry  288  17895-907  2013

      Show Abstract
      23645681 23645681
      Phosphorylation of KRAB-associated protein 1 (KAP1) at Tyr-449, Tyr-458, and Tyr-517 by nuclear tyrosine kinases inhibits the association of KAP1 and heterochromatin protein 1α (HP1α) with heterochromatin.
      Kubota, S; Fukumoto, Y; Aoyama, K; Ishibashi, K; Yuki, R; Morinaga, T; Honda, T; Yamaguchi, N; Kuga, T; Tomonaga, T; Yamaguchi, N
      The Journal of biological chemistry  288  17871-83  2013

      Show Abstract
      23645696 23645696
      Differentiation-independent fluctuation of pluripotency-related transcription factors and other epigenetic markers in embryonic stem cell colonies.
      Sustáčková, G; Legartová, S; Kozubek, S; Stixová, L; Pacherník, J; Bártová, E
      Stem cells and development  21  710-20  2012

      Show Abstract
      21609209 21609209
      An epigenetic silencing pathway controlling T helper 2 cell lineage commitment.
      Allan, Rhys S, et al.
      Nature, 487: 249-53 (2012)  2012

      Show Abstract
      22763435 22763435

      FAQ

      QuestionAnswer
      I would like to use this monoclonal antibody on mouse tissue, are there any special procedures I should follow?To reduce the background interaction between the monoclonal antibody and the mouse tissue, you will need to perform antigen retrieval and use a special secondary antibody. For antigen retrieval, prepare a citrate buffer by mixing 19ml of 0.1M citric acid and 82ml of 0.1M sodium citrate to make 1000ml of buffer. Adjust the pH to 6.0 before each use. Add buffer to your slide. Using an 800 watt microwave oven with rotation, microwave for 7.5 min at high, 5 min at 50% (3 cycles), checking the slide at the end of each cycle to see if the solution needs to be replaced, adding water if needed. For the secondary antibody, use a biotinylated isotype specific secondary IGG (IGG-1) and a 3% donkey serum block. This will help reduce the background issues using mouse monoclonals.

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      MAB3446 Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 Show Pricing & Availability
      MAB3584 Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5 Show Pricing & Availability
      MAB3866 Anti-Heterochromatin Antibody Show Pricing & Availability
      MABE479 Anti-HP1 alpha, clone EPR5777, Rabbit Monoclonal Antibody Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies