05-949 | Anti-Histidine Tagged Antibody, clone HIS.H8

05-949
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-949
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histidine Tagged Antibody, clone HIS.H8
      Background InformationHis-Tag is a series of at least 6 histidine residues used as a tag motif at the N- or C-terminus of recombinant proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. Its small size also makes it more suitable for use under denaturing conditions. His tags are most often used for affinity purification and binding assays. His tags bind to nickel or cobalt ions with high affinity, and this interaction with metal ions is the basis of many his-tag based affinity media. The His Tag epitope is widely used to detect expression of recombinant proteins in bacterial, yeast, insect,and mammalian systems.
      References
      Product Information
      FormatPurified
      Control
      • RIPA lysates of transfected HeLa cells.
      PresentationPurified mouse monoclonal IgG2b in buffer containing PBS, pH 7.4 with 0.05% sodium azide.
      Applications
      ApplicationThis Anti-Histidine Tagged Antibody, clone HIS.H8 is validated for use in IC, IP, WB for the detection of Histidine Tagged.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry:
      2 μg/mL of a previous lot of antibody showed positive immunostaining for His tagged Akt1/PKBα in transfected HeLa cells.

      Immunoprecipitation:
      This antibody has been reported by an independent laboratory to immunoprecipitate His Tagged proteins.
      Biological Information
      Immunogen6x His synthetic peptide.
      CloneHIS.H8
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes tag sequence HHHHHH on either the N- or C-terminus of any recombinant fusion protein; no other amino acids surrounding the 6x-His tag sequence are required for the recognition of the 6x-His tag.
      IsotypeIgG2b
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Purification MethodPurified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot and immunocytochemistry on transfected HeLa cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected His tagged Akt1/PKBα in RIPA lysates of transfected HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations:
      Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-His Tag, clone HIS.H8 2474838
      Anti-His Tag, clone HIS.H8 (mouse monoclonal IgG2b) - DAM1413921 DAM1413921
      Anti-His Tag, clone HIS.H8 - 2364898 2364898
      Anti-His Tag, clone HIS.H8 - 2387750 2387750
      Anti-His Tag, clone HIS.H8 - 2426469 2426469
      Anti-His Tag, clone HIS.H8 - 0606032212 0606032212
      Anti-His Tag, clone HIS.H8 - 0607035977 0607035977
      Anti-His Tag, clone HIS.H8 - 0609040561 0609040561
      Anti-His Tag, clone HIS.H8 - 0701050932 0701050932
      Anti-His Tag, clone HIS.H8 - 1958321 1958321

      References

      Reference overviewPub Med ID
      Nuclease activity of Legionella pneumophila Cas2 promotes intracellular infection of amoebal host cells.
      Gunderson, FF; Mallama, CA; Fairbairn, SG; Cianciotto, NP
      Infection and immunity  83  1008-18  2015

      Show Abstract
      25547789 25547789
      Directed evolution of anti-HER2 DARPins by SNAP display reveals stability/function trade-offs in the selection process.
      Houlihan, G; Gatti-Lafranconi, P; Lowe, D; Hollfelder, F
      Protein engineering, design & selection : PEDS  28  269-79  2015

      Show Abstract
      26134501 26134501
      Acetylation limits 53BP1 association with damaged chromatin to promote homologous recombination.
      Tang, Jiangbo, et al.
      Nat. Struct. Mol. Biol., 20: 317-25 (2013)  2013

      Show Abstract
      23377543 23377543
      Enzyme replacement therapy rescues weakness and improves muscle pathology in mice with X-linked myotubular myopathy.
      Lawlor, MW; Armstrong, D; Viola, MG; Widrick, JJ; Meng, H; Grange, RW; Childers, MK; Hsu, CP; O'Callaghan, M; Pierson, CR; Buj-Bello, A; Beggs, AH
      Human molecular genetics  22  1525-38  2013

      Show Abstract
      23307925 23307925
      ATX1-generated H3K4me3 is required for efficient elongation of transcription, not initiation, at ATX1-regulated genes.
      Ding, Y; Ndamukong, I; Xu, Z; Lapko, H; Fromm, M; Avramova, Z
      PLoS genetics  8  e1003111  2012

      Show Abstract
      23284292 23284292
      Interplay between REST and nucleolin transcription factors: a key mechanism in the overexpression of genes upon increased phosphorylation.
      Tediose, Teeo, et al.
      Nucleic acids research, (2010)  2010

      Show Abstract
      20100803 20100803
      Upregulating CD59: a new strategy for protection of neurons from complement-mediated degeneration.
      M V Kolev,T Tediose,B Sivasankar,C L Harris,J Thome,B P Morgan,R M Donev
      The pharmacogenomics journal  10  2010

      Show Abstract
      19884909 19884909
      Solution structure of a novel zinc finger motif in the SAP30 polypeptide of the Sin3 corepressor complex and its potential role in nucleic acid recognition.
      He, Y; Imhoff, R; Sahu, A; Radhakrishnan, I
      Nucleic acids research  37  2142-52  2009

      Show Abstract
      19223330 19223330
      Thrombospondin-related adhesive protein (TRAP) of Plasmodium falciparum: expression during sporozoite ontogeny and binding to human hepatocytes.
      Robson, K J, et al.
      EMBO J., 14: 3883-94 (1995)  1995

      Show Abstract
      7664729 7664729

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      Alternative Packsize

      Catalogue Number Description  
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies