07-627 | Anti-Histone H2A.X Antibody

200 µL  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HPIA, WBRbSerumPolyclonal Antibody
      Catalogue Number07-627
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H2A.X Antibody
      Alternate Names
      • H2A histone family, member X
      • H2AX histone
      Background InformationH2AX is involved in DNA repair and the maintenance of genomic stability. although its precise role is still to be defined. H2AX is phosphorylated soon after the appearance of double-stranded breaks. and has been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis.
      Product Information
      • HeLa acid extracts
      PresentationRabbit antiserum containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C.
      ApplicationAnti-Histone H2A.X Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A.X also known as H2A histone family member X, H2AX histone & has been validated in PIA & WB.
      Key Applications
      • Peptide Inhibition Assay
      • Western Blotting
      Application NotesPeptide Inhibition Analysis:
      1 and 10 μM of the immunizing histone H2A.X peptide abolished detection of histone H2A.X by a previous lot in immunoblot analysis of HeLa acid extracts.
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide (CSATVGPKAPSGGKKA) corresponding to amino acids 121-135 of human histone H2A.X, with an N-terminal cysteine added for conjugation purposes. The immunizing sequence has 13/15 identical amino acids in mouse.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityRecognizes histone H2A.X, Mr 15 kDa. A band at Mr 100 kDa was also observed.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
      Gene Symbol
      • H2AFX
      • H2A/X
      • H2A.X
      • H2AX
      • H2a/x
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION:SwissProt: P16104 # Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
      SIZE: 143 amino acids; 15145 Da
      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with numerous proteins required for DNA damage signaling and repair when phosphorylated on Ser-140. These include MDC1, TP53BP1, BRCA1 and the MRN complex, composed of MRE11A, RAD50, and NBN. Interaction with the MRN complex is mediated at least in part by NBN. Also interacts with DHX9/NDHII when phosphorylated on Ser-140.
      SUBCELLULAR LOCATION: Nucleus.DEVELOPMENTAL STAGE: Synthesized in G1 as well as in S-phase.
      DOMAIN: SwissProt: P16104 The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
      PTM: Phosphorylated on Ser-140 (to form gamma-H2AFX) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, and may also occur during meiotic recombination events and immunoglobulin class switching in lymphocytes. Phosphorylation can extend up to several thousand nucleosomes from the actual site of the DSB and may mark the surrounding chromatin for recruitment of proteins required for DNA damage signaling and repair. Widespread phosphorylation may also serve to amplify the damage signal or aid repair of persistent lesions. Phosphorylation of Ser-140 in response to ionizing radiation is mediated by both ATM and PRKDC while defects in DNA replication induce Ser-140 phosphorylation subsequent to activation of ATR and PRKDC. Dephosphorylation of Ser-140 by PP2A is required for DNA DSB repair. In meiosis, Ser-140 phosphorylation may occur at synaptonemal complexes during leptotene as an ATM-dependent response to the formation of programmed DSBs by SPO11. Ser-140 phosphorylation may subsequently occurs at unsynapsed regions of both autosomes and the XY bivalent during zygotene, downstream of ATR and BRCA1 activation. Ser-140 phosphorylation may also be required for transcriptional repression of unsynapsed chromatin and meiotic sex chromosome inactivation (MSCI), whereby the X and Y chromosomes condense in pachytene to form the heterochromatic XY-body. During immunoglobulin class switch recombination in lymphocytes, Ser-140 phosphorylation may occur at sites of DNA-recombination subsequent to activation of the activation-induced cytidine deaminase AICDA. & Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression (By similarity).
      SIMILARITY: Belongs to the histone H2A family. ... hide » see more » Entrez Gene Number: NP_002096 Entrez Gene Summary Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
      Molecular Weight15 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot of acid-extracted proteins from HeLa cells.

      Western Blot Analysis:
      A 1:2,000 - 1:10,000 dilution of this lot detected histone H2A.X in acid-extracted proteins from HeLa cells. The antibody did not detect recombinant Histone H2A (Catalog # 14-493).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H2A.X - 2455636 2455636
      Anti-Histone H2A.X - 1977378 1977378
      Anti-Histone H2A.X - 2066059 2066059
      Anti-Histone H2A.X - 2295662 2295662
      Anti-Histone H2A.X - 2363003 2363003
      Anti-Histone H2A.X - 27076 27076
      Anti-Histone H2A.X - 31783 31783
      Anti-Histone H2A.X - DAM1394817 DAM1394817
      Anti-Histone H2A.X - DAM1543270 DAM1543270
      Anti-Histone H2A.X - DAM1601842 DAM1601842


      Reference overviewApplicationPub Med ID
      SWI/SNF complexes are required for full activation of the DNA-damage response.
      Smith-Roe, SL; Nakamura, J; Holley, D; Chastain, PD; Rosson, GB; Simpson, DA; Ridpath, JR; Kaufman, DG; Kaufmann, WK; Bultman, SJ
      Oncotarget  6  732-45  2015

      Show Abstract
      Western Blotting25544751 25544751
      A kinome-targeted RNAi-based screen links FGF signaling to H2AX phosphorylation in response to radiation.
      Benzina, S; Pitaval, A; Lemercier, C; Lustremant, C; Frouin, V; Wu, N; Papine, A; Soussaline, F; Romeo, PH; Gidrol, X
      Cellular and molecular life sciences : CMLS  72  3559-73  2015

      Show Abstract
      25894690 25894690
      DNA Damage Signaling Is Induced in the Absence of Epstein-Barr Virus (EBV) Lytic DNA Replication and in Response to Expression of ZEBRA.
      Wang'ondu, R; Teal, S; Park, R; Heston, L; Delecluse, H; Miller, G
      PloS one  10  e0126088  2015

      Show Abstract
      25950714 25950714
      Critical role of lysine 134 methylation on histone H2AX for γ-H2AX production and DNA repair.
      Sone, K; Piao, L; Nakakido, M; Ueda, K; Jenuwein, T; Nakamura, Y; Hamamoto, R
      Nature communications  5  5691  2014

      Show Abstract
      Western Blotting, Immunohistochemistry, Immunocytochemistry25487737 25487737
      An RNF168 fragment defective for focal accumulation at DNA damage is proficient for inhibition of homologous recombination in BRCA1 deficient cells.
      Muñoz, MC; Yanez, DA; Stark, JM
      Nucleic acids research  42  7720-33  2014

      Show Abstract
      Western Blotting24829461 24829461
      Telomerase inhibitor Imetelstat (GRN163L) limits the lifespan of human pancreatic cancer cells.
      Burchett, KM; Yan, Y; Ouellette, MM
      PloS one  9  e85155  2014

      Show Abstract
      24409321 24409321
      Selective inhibition of p300 HAT blocks cell cycle progression, induces cellular senescence, and inhibits the DNA damage response in melanoma cells.
      Yan, G; Eller, MS; Elm, C; Larocca, CA; Ryu, B; Panova, IP; Dancy, BM; Bowers, EM; Meyers, D; Lareau, L; Cole, PA; Taverna, SD; Alani, RM
      The Journal of investigative dermatology  133  2444-52  2013

      Show Abstract
      23698071 23698071
      Mouse tissues that undergo neoplastic progression after K-Ras activation are distinguished by nuclear translocation of phospho-Erk1/2 and robust tumor suppressor responses.
      Parikh, N; Shuck, RL; Nguyen, TA; Herron, A; Donehower, LA
      Molecular cancer research : MCR  10  845-55  2012

      Show Abstract
      Immunohistochemistry22532587 22532587
      Histone lysine methyltransferase SETD8 promotes carcinogenesis by deregulating PCNA expression.
      Takawa, M; Cho, HS; Hayami, S; Toyokawa, G; Kogure, M; Yamane, Y; Iwai, Y; Maejima, K; Ueda, K; Masuda, A; Dohmae, N; Field, HI; Tsunoda, T; Kobayashi, T; Akasu, T; Sugiyama, M; Ohnuma, S; Atomi, Y; Ponder, BA; Nakamura, Y; Hamamoto, R
      Cancer research  72  3217-27  2012

      Show Abstract
      Western Blotting22556262 22556262
      Synthetic lethality of PARP inhibition in BRCA-network disrupted tumor cells is associated with interferon pathway activation and enhanced by interferon-γ.
      Paul Warrener,Sammy Kim,Sybil M G Williams,Matthew Biery,Marcia Gordon,Carlo Toniatti,Michele A Cleary,Peter S Linsley,Michael Carleton
      Apoptosis : an international journal on programmed cell death  17  2012

      Show Abstract
      22392482 22392482

      Technical Info

      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

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