06-755 | Anti-Histone H3 Antibody

06-755
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWB, ICC, IP, ChIPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-755
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Histone H3 Antibody
      Alternate Names
      • Histone H3.1t
      • H3/t
      • H3t
      • H3/g
      Background InformationHistone H3, also known as Histone H3.1t, H3/t, H3t, or H3/g, and encoded by the gene HIST3H3/H3Ft, is one of the core components of the nucleosome. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The octamer wraps approximately 147 bp of DNA. A classic nuclear marker, new expression is seen during S phase of the cell cycle. Histone H3 is often acetylated and or methylated multiple times. Changes in methylation or acetylation state of histone H3 together with similar modification on the sister histones is one key aspect of epigenetic gene expression and regulation.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa acid extract
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Histone H3 Antibody is a Rabbit Polyclonal Antibody for detection of Histone H3 also known as histone 3-H3 & has been validated in ChIP, ICC & WB.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Histone H3 in HeLa cells.

      Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Histone H3 from 500 µg of HeLa acid extract. Immunoprecipitated sample was then subjected to Western Blotting using 0.5 µg/mL of the same antibody.

      Chromatin Immunoprecipitaton: A representative lot from an independent laboratory immunoprecipitated Histone H3 from plant chromatin (Ng, D. W., et al. (2006). Plant Cell. 18(1):119-132.).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Histone H3.
      EpitopeN-terminus
      Concentration0.5 mg/mL
      HostRabbit
      SpecificityThis antibody recognizes Histone H3 at the N-terminus.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Broad species cross-reactivity is expected based on sequence similarity.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~17 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa acid extract.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Histone H3 in 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H3 2477008
      Anti-Histone H3 ANTI-HISTONE H3
      Anti-Histone H3 - 2424922 2424922
      Anti-Histone H3 - 2442218 2442218
      Anti-Histone H3 - 2450206 2450206
      Anti-Histone H3 - 16965 16965
      Anti-Histone H3 - 19231 19231
      Anti-Histone H3 - 1953330 1953330
      Anti-Histone H3 - 19713 19713
      Anti-Histone H3 - 1992451 1992451

      References

      Reference overviewApplicationSpeciesPub Med ID
      Multimer Formation Explains Allelic Suppression of PRDM9 Recombination Hotspots.
      Baker, CL; Petkova, P; Walker, M; Flachs, P; Mihola, O; Trachtulec, Z; Petkov, PM; Paigen, K
      PLoS genetics  11  e1005512  2015

      Show Abstract
      26368021 26368021
      Mutation of histone H3 serine 86 disrupts GATA factor Ams2 expression and precise chromosome segregation in fission yeast.
      Lim, KK; Ong, TY; Tan, YR; Yang, EG; Ren, B; Seah, KS; Yang, Z; Tan, TS; Dymock, BW; Chen, ES
      Scientific reports  5  14064  2015

      Show Abstract
      26369364 26369364
      Drosophila casein kinase I alpha regulates homolog pairing and genome organization by modulating condensin II subunit Cap-H2 levels.
      Nguyen, HQ; Nye, J; Buster, DW; Klebba, JE; Rogers, GC; Bosco, G
      PLoS genetics  11  e1005014  2015

      Show Abstract
      25723539 25723539
      Targeted repression of AXIN2 and MYC gene expression using designer TALEs.
      Rennoll, SA; Scott, SA; Yochum, GS
      Biochemical and biophysical research communications  446  1120-5  2014

      Show Abstract
      24667606 24667606
      Histone acetylation associated up-regulation of the cell wall related genes is involved in salt stress induced maize root swelling.
      Li, H; Yan, S; Zhao, L; Tan, J; Zhang, Q; Gao, F; Wang, P; Hou, H; Li, L
      BMC plant biology  14  105  2014

      Show Abstract
      Immunofluorescence24758373 24758373
      An ARID domain-containing protein within nuclear bodies is required for sperm cell formation in Arabidopsis thaliana.
      Zheng, B; He, H; Zheng, Y; Wu, W; McCormick, S
      PLoS genetics  10  e1004421  2014

      Show Abstract
      25057814 25057814
      Histones to the cytosol: exportin 7 is essential for normal terminal erythroid nuclear maturation.
      Hattangadi, SM; Martinez-Morilla, S; Patterson, HC; Shi, J; Burke, K; Avila-Figueroa, A; Venkatesan, S; Wang, J; Paulsen, K; Görlich, D; Murata-Hori, M; Lodish, HF
      Blood  124  1931-40  2014

      Show Abstract
      25092175 25092175
      Involvement of telomerase reverse transcriptase in heterochromatin maintenance.
      Maida, Y; Yasukawa, M; Okamoto, N; Ohka, S; Kinoshita, K; Totoki, Y; Ito, TK; Minamino, T; Nakamura, H; Yamaguchi, S; Shibata, T; Masutomi, K
      Molecular and cellular biology  34  1576-93  2014

      Show Abstract
      Western Blotting24550003 24550003
      The conserved PHD1-PHD2 domain of ZFP-1/AF10 is a discrete functional module essential for viability in Caenorhabditis elegans.
      Avgousti, DC; Cecere, G; Grishok, A
      Molecular and cellular biology  33  999-1015  2013

      Show Abstract
      23263989 23263989
      FOSL1 controls the assembly of endothelial cells into capillary tubes by direct repression of αv and β3 integrin transcription.
      Evellin, S; Galvagni, F; Zippo, A; Neri, F; Orlandini, M; Incarnato, D; Dettori, D; Neubauer, S; Kessler, H; Wagner, EF; Oliviero, S
      Molecular and cellular biology  33  1198-209  2013

      Show Abstract
      Western Blotting, Immunofluorescence23319049 23319049

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