05-172 | Anti-IGF-I Antibody, clone Sm1.2

05-172
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Ch, AvIHC, IP, NEUT, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-172
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IGF-I Antibody, clone Sm1.2
      Alternate Names
      • Mechano growth factor
      • insulin-like growth factor 1
      • insulin-like growth factor 1 (somatomedin C)
      • somatomedin C
      Background InformationInsulin-like growth factor 1 (IGF-1) is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. IGF-1 consists of 70 amino acids in a single chain with three intramolecular disulfide bridges.
      IGF-1 is produced primarily by the liver as an endocrine hormone and targets tissues in a paracrine/autocrine fashion. Production is stimulated by growth hormone and can be retarded by undernutrition, growth hormone insensitivity, lack of growth hormone receptors, or failures of the downstream signaling pathway post GH receptor including SHP2 and STAT5b. Approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). IGFBP-3, the most abundant protein, accounts for 80% of all IGF binding. IGF-1 binds to IGFBP-3 in a 1:1 molar ratio. Its primary action is mediated by binding to specific IGF receptors present on many cell types in many tissues. The signal is transduced by intracellular events. IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
      References
      Product Information
      FormatPurified
      Control
      • MCF-7 cell extracts
      PresentationPurified mouse IgG1κ lyophilized powder, lyophilized from 105 μL of 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA.
      Applications
      ApplicationAnti-IGF-I Antibody, clone Sm1.2 is an antibody against IGF-I for use in IH, IP, NEUT & WB.
      Key Applications
      • Immunohistochemistry
      • Immunoprecipitation
      • Neutralizing
      • Western Blotting
      Application NotesImmunoprecipitation:
      5 μg of a previous lot immunoprecipitated 100 ng of IGF-I.

      Immunohistochemistry:
      10 μg/mL of a previous lot was reported by an independent laboratory to detect IFG-I in formalin-fixed, paraffin-embedded skin sections.
      (Lui, 1994).

      Neutralization:
      10-20 μg/mL of a previous lot inhibited the activity of 10 ng/mL of IGF-I as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay (ATPLiteTM-M, Packard Instruments).
      Biological Information
      ImmunogenHuman insulin-like growth factor I (IGF-I) purified from human plasma. (van Wyk, J.J., 1986).
      CloneSm1.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityIGF-I. (Russel, W.E., 1984). 40% cross reactivity to IGF-II. (van Wyk, J.J., 1997).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Chicken
      • Avian
      Species Reactivity NoteHuman, mouse, rat and possibly chicken.
      Occasional difficulty neutralizing rat IGF-I due to binding protein interference.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Several transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • IGF1
      • IBP1
      • IGFI
      • IGF-IA
      • MGF
      • IGF-IB
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05019 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.| P01343 # The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity.
      SIZE: 195 amino acids; 21841 Da
      SUBCELLULAR LOCATION: Secreted.
      DOMAIN: SwissProt: P05019
      DISEASE: SwissProt: P05019 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.| P01343 # Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
      SIMILARITY: SwissProt: P05019 ## Belongs to the insulin family.| P01343 ## Belongs to the insulin family.
      Molecular Weight17 kDa, migrates to ~7 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated on IGF-I (Catalog # 01-208) under non-reducing conditions.

      Western Blot Analysis:
      0.5-2 μg/mL of this antibody detected 100 ng of IGF-I (Catalog # 01-208) under non-reducing conditions.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IGF-I, clone Sm1.2 -2664249 2664249
      Anti-IGF-I, clone Sm1.2 - 2375605 2375605
      Anti-IGF-I, clone Sm1.2 - 2433501 2433501
      Anti-IGF-I, clone Sm1.2 - 12066 12066
      Anti-IGF-I, clone Sm1.2 - 19667 19667
      Anti-IGF-I, clone Sm1.2 - 26433 26433
      Anti-IGF-I, clone Sm1.2 - 27835 27835
      Anti-IGF-I, clone Sm1.2 -2514063 2514063
      Anti-IGF-I, clone Sm1.2 -2649105 2649105
      Anti-IGF-I, clone Sm1.2 -2717625 2717625

      References

      Reference overviewApplicationPub Med ID
      The transcription factors Ik-1 and MZF1 downregulate IGF-IR expression in NPM-ALK⁺ T-cell lymphoma.
      Vishwamitra, D; Curry, CV; Alkan, S; Song, YH; Gallick, GE; Kaseb, AO; Shi, P; Amin, HM
      Molecular cancer  14  53  2015

      Show Abstract
      25884514 25884514
      Age-related macular degeneration-associated silent polymorphisms in HtrA1 impair its ability to antagonize insulin-like growth factor 1.
      Jacobo, SM; Deangelis, MM; Kim, IK; Kazlauskas, A
      Molecular and cellular biology  33  1976-90  2013

      Show Abstract
      Western Blotting23478260 23478260
      Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury.
      Sato, A; Ohtaki, H; Tsumuraya, T; Song, D; Ohara, K; Asano, M; Iwakura, Y; Atsumi, T; Shioda, S
      Journal of neuroinflammation  9  65  2012

      Show Abstract
      22483094 22483094
      PTH ameliorates acidosis-induced adverse effects in skeletal growth centers: the PTH-IGF-I axis.
      Green, J; Goldberg, R; Maor, G
      Kidney international  63  487-500  2003

      Show Abstract
      12631114 12631114
      Growth factors and stromal matrix proteins associated with mammographic densities.
      Y P Guo, L J Martin, W Hanna, D Banerjee, N Miller, E Fishell, R Khokha, N F Boyd
      Cancer epidemiology, biomarkers prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology  10  243-8  2001

      Show Abstract
      11303594 11303594
      The matrix metalloproteinase-9 regulates the insulin-like growth factor-triggered autocrine response in DU-145 carcinoma cells
      Manes, S., et al
      J Biol Chem, 274:6935-45 (1999)  1999

      Immunoprecipitation10066747 10066747
      Parathyroid hormone-(1-34) enhances aggrecan synthesis via an insulin-like growth factor-I pathway
      Harvey, A. K., et al
      J Biol Chem, 274:23249-55 (1999)  1999

      Immunoblotting (Western)10438499 10438499
      Sex hormone-induced prostatic carcinogenesis in the noble rat: the role of insulin-like growth factor-I (IGF-I) and vascular endothelial growth factor (VEGF) in the development of prostate cancer.
      Y Z Wang, Y C Wong
      The Prostate  35  165-77  1998

      Show Abstract
      9582085 9582085
      Parathyroid hormone exerts disparate effects on osteoblast differentiation depending on exposure time in rat osteoblastic cells
      Ishizuya, T., et al
      J Clin Invest, 99:2961-70 (1997)  1997

      Neutralization Assay9185520 9185520
      Cryogenic spinal cord injury induces astrocytic gene expression of insulin-like growth factor I and insulin-like growth factor binding protein 2 during myelin regeneration
      Yao, D. L., et al
      J Neurosci Res, 40:647-59 (1995)  1995

      Immunohistochemistry (Tissue)7541476 7541476

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      Alternative Packsize

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies