05-166 Anti-IGF-II Antibody, clone S1F2

500 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RELISA, IP, ICC, NEUT, WBMPurifiedMonoclonal Antibody
      Catalogue Number05-166
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IGF-II Antibody, clone S1F2
      Background InformationHuman Insulin-like Growth Factor II (IGFII) contains 67 amino acids and shares similar structural features with IGFI, including a 62% sequence homology. In human plasma, IGFI and IGFII are associated with IGF-binding proteins that transport the polypeptides and partially regulate their actions in vivo. In addition to the insulin receptor, IGFII binds to two forms of IGF receptors, both of which are widely distributed in different tissues and cultured cells. IGFII is mitogenic for a variety of cultured cells, including mouse 3T3 cells,12 normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells.
      Product Information
      • Human head and neck tumours, angioma tissues
      PresentationPurified mouse monoclonal IgG1 in buffer containing PBS, pH 7.4., purified by ammonium sulfate precipitation and DEAE-cellulose chromatography. Frozen solution.
      ApplicationDetect IGF-II using this Anti-IGF-II Antibody, clone S1F2 validated for use in ELISA, IP, IC, NEUT & WB.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Immunocytochemistry
      • Neutralizing
      • Western Blotting
      Application NotesNeutralization: 10 μg/mL of a previous lot inhibited the stimulatory effects of IGF-II (10 ng/mL) on DNA synthesis in chicken embryo fibroblasts. Cell viability was assessed using an ATP endpoint assay (ATPLiteÔ-M, Packard Instruments). Previous lots of antibody were tested to inhibit IGF-II-dependent incorporation of [3H]-thymidine
      Biological Information
      ImmunogenPartially purified rat IGF-II. Clone S1F2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody detects IGF-II.
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteRecognizes rat and human IGF-II. Less than 10% cross-reactivity to human IGF-I (Tanaka, H, 1989; Suzuki, T, 1989).
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
      Gene Symbol
      • IGF2
      • FLJ22066
      • FLJ44734
      • pp9974
      • IGF-II
      • INSIGF
      • C11orf43
      Purification MethodAmmonium sulfate precipitation and DEAE-cellulose chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01344 # The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
      SIZE: 180 amino acids; 20140 Da
      SIMILARITY: SwissProt: P01344 ## Belongs to the insulin family.
      Molecular Weight~7 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting on HeLa acid extracted nuclear preps (positive) and recombinant Histone H3 (negative).

      Western Blotting Analysis:
      1:5,000 – 1:10,000 dilution of this antibody was used to detect trimethylated-Histone H3 (Lys4) in HeLa nuclear acid extracts.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size500 µg
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-Histone H3 (Unmodified Lys4), clone CMA301 -26811972681197
      Anti-IGF-II, clone S1F23068488
      Anti-IGF-II, clone S1F2 - 23877492387749
      Anti-IGF-II, clone S1F2 - 06090396760609039676
      Anti-IGF-II, clone S1F2 - 1619616196
      Anti-IGF-II, clone S1F2 - 1686316863
      Anti-IGF-II, clone S1F2 - 1823418234
      Anti-IGF-II, clone S1F2 - 1895618956
      Anti-IGF-II, clone S1F2 - 19595571959557
      Anti-IGF-II, clone S1F2 - 1966219662


      Reference overviewApplicationPub Med ID
      Maternal protein restriction regulates IGF2 system in placental labyrinth.
      Gao, H; Sathishkumar, KR; Yallampalli, U; Balakrishnan, M; Li, X; Wu, G; Yallampalli, C
      Frontiers in bioscience (Elite edition)  4  1434-50  2012

      Show Abstract
      Western Blotting22201967 22201967
      Molecular analysis of non-small cell lung cancer identifies subsets with different sensitivity to insulin-like growth factor I receptor inhibition.
      Gualberto, A; Dolled-Filhart, M; Gustavson, M; Christiansen, J; Wang, YF; Hixon, ML; Reynolds, J; McDonald, S; Ang, A; Rimm, DL; Langer, CJ; Blakely, J; Garland, L; Paz-Ares, LG; Karp, DD; Lee, AV
      Clinical cancer research : an official journal of the American Association for Cancer Research  16  4654-65  2010

      Show Abstract
      20670944 20670944
      Altered nephrogenesis due to maternal diabetes is associated with increased expression of IGF-II/mannose-6-phosphate receptor in the fetal kidney
      Amri, K., et al
      Diabetes, 50:1069-75 (2001)  2001

      Immunoblotting (Western)11334410 11334410
      Pediatric adrenocortical tumors: molecular events leading to insulin-like growth factor II gene overexpression.
      Wilkin, F, et al.
      J. Clin. Endocrinol. Metab., 85: 2048-56 (2000)  2000

      Show Abstract
      Immunohistochemistry (Tissue)10843195 10843195
      Parathyroid hormone-(1-34) enhances aggrecan synthesis via an insulin-like growth factor-I pathway
      Harvey, A. K., et al
      J Biol Chem, 274:23249-55 (1999)  1999

      Immunoblotting (Western)10438499 10438499
      Post-translational processing of the insulin-like growth factor-2 precursor. Analysis of O-glycosylation and endoproteolysis
      Duguay, S. J., et al
      J Biol Chem, 273:18443-51 (1998)  1998

      Immunofluorescence9660813 9660813
      A putative sub-10-kDa basement membrane activity required for lung alveolar formation in vitro.
      M L Matter, G W Laurie
      The American journal of physiology  271  L489-94  1996

      Show Abstract
      8843800 8843800
      Combined magnetic fields increase insulin-like growth factor-II in TE-85 human osteosarcoma bone cell cultures.
      R J Fitzsimmons, J T Ryaby, S Mohan, F P Magee, D J Baylink
      Endocrinology  136  3100-6  1995

      Show Abstract
      7789337 7789337
      Identification of a family of insulin-like growth factor II secreted by cultured rat epithelial-like cell line 18,54-SF: application of a monoclonal antibody
      Tanaka, H, et al
      Endocrinology, 124:870-7 (1989)  1989

      2463906 2463906
      Immunocytochemical demonstration of IGF-II-like immunoreactivity in human paraganglioma of the craniocervical region
      Suzuki, T, et al
      Virchows Archiv A, Pathological anatomy and histopathology, 414:515-21 (1989)  1989

      2499109 2499109

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