05-1106 | Anti-IGF-IR Antibody, β-subunit, clone 1-2

05-1106
100 µg  
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      Overview

      Replacement Information
      Replacement Information05-1106 is a recommended replacement for MAB1123

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWB, IH(P)MAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue Number05-1106
      ReplacesMAB1123
      DescriptionAnti-IGF-IR Antibody, β-subunit, clone 1-2
      Background InformationIGF-I and -II signal through the IGF-I Receptor, which is homologous to the Insulin Receptor. The high-affinity IGF-II Receptor does not play a direct role in signaling, but regulates the concentration of free IGF-II. The IGFs are involved in skeletal growth, and are essential for prevention of apoptosis. Serum levels of free IGFs are kept low by the action of IGF binding proteins (IGFBPs), which sequester the IGFs. Overexpression of IGFBPs may induce apoptosis, presumably by reduction of free IGF; IGFBP levels are also altered in some cancers. The IGF-I Receptor is not as mitogenic as some other growth factor receptors, but its ability to activate the PI3 Kinase pathway, through the Insulin Receptor Substrate (IRS) proteins, is critical for mediating cell survival.
      References
      Product Information
      FormatAffinity Purified
      Control
      • C2C12 Cell Lysate
      PresentationPurified mouse monoclonal IgG2b in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.
      Applications
      ApplicationAnti-IGF-IR Antibody, β-subunit, clone 1-2 is an antibody against IGF-IR for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry(paraffin): Representative images from a previous lot.
      Optimal Staining of IGF Monoclonal Antibody: Placenta

      Western Blot: A previous lot of this antibody was used at 1-2 µg/mL for 2 hours at room temperature.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA 15-mer peptide corresponding to a.a. 1323-1337 (Tyr-RKNERALPLPQSSTC) from the C-terminal of β-subunit of human IGF-1R.
      EpitopeBetween a.a. 1323-1337 of IGF-1R
      Clone1-2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes the β-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 1323-1337 of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the α- and β-subunits, which are disulfide-linked in a β-α-α-β configuration in the mature receptor. The α-subunit is completely extracellular, while the β-subunit spans membrane.
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteMouse and rat. Expected to react with human based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq]

      Gene Symbol
      • IGF1R
      • MGC142170
      • MGC18216
      • IGFIR
      • JTK13
      • MGC142172
      • CD221
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade.
      CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
      ENZYME REGULATION: Autophosphorylation activates the kinase activity.
      SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Expressed in a variety of tissues.
      PTM: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
      PTM: Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
      DISEASE: Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
      SIMILARITY: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
      SIMILARITY: Contains 3 fibronectin type-III domains.
      SIMILARITY: Contains 1 protein kinase domain.
      Molecular WeightRecognizes the beta-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R; ~140 kDa)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on C2C12 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected IGF-1 Receptor beta on 10 μg of C2C12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IGF-1R, -subunit, clone 1-2 - 1978007 1978007
      Anti-IGF-1R, -subunit, clone 1-2 - 2001253 2001253
      Anti-IGF-1R, -subunit, clone 1-2 - 2070000 2070000
      Anti-IGF-1R, -subunit, clone 1-2 - 2154090 2154090
      Anti-IGF-1R, -subunit, clone 1-2 - 2297319 2297319
      Anti-IGF-1R, -subunit, clone 1-2 - NG1576252 NG1576252
      Anti-IGF-1R, -subunit, clone 1-2 - NG1576253 NG1576253
      Anti-IGF-1R, -subunit, clone 1-2 - NG1640808 NG1640808
      Anti-IGF-1R, -subunit, clone 1-2 - NG1800808 NG1800808
      Anti-IGF-1R, -subunit, clone 1-2 - NG1853757 NG1853757

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies