05-535 | Anti-IKKβ Antibody, clone 10AG2

05-535
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-535
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IKKβ Antibody, clone 10AG2
      Alternate Names
      • I-kappa-B kinase 2
      • I-kappa-B-kinase beta
      • Nuclear factor NF-kappa-B inhibitor kinase beta
      • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta
      • inhibitor of nuclear factor kappa B kinase beta subunit
      Background InformationIKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1.
      References
      Product Information
      FormatPurified
      Control
      • Jurkat cell lysate and human cervical carcinoma.
        Included Positive Antigen Control: Catalog # 12-303, Jurkat cell extract. Use 20 µg per lane for minigels.
      PresentationPurified mouse monoclonal IgG1 in buffer containing PBS containing 0.05% BSA, with 0.05% sodium azide. Frozen solution.
      Applications
      ApplicationAnti-IKKβ Antibody, clone 10AG2 detects level of IKKβ & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenHis-tagged, full length human IKKβ/IKK2. Clone: 10AG2.
      Clone10AG2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes IKKβ/IKK2, Mr 87 kDa.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteProven to react with human, mouse and rat. Other species not tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryNFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
      Gene Symbol
      • IKBKB
      • IKK-beta
      • NFKBIKB
      • IKK2
      • FLJ40509
      • IKKB
      • MGC131801
      • IKK-B
      • IkBKB
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O14920 # Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3 (By similarity).
      SIZE: 756 amino acids; 86564 Da
      SUBUNIT: Preferentially found as a heterodimer with IKK-alpha but also as a homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. The tripartite complex can also bind to MEKK1, MAP3K14/NIK, IKAP and IKB-alpha-p65-p50 complex. Phosphorylated IKB-alpha is further released from the complex. Found in a complex composed of NCOA2, NCOA3, IKKA, IKBKG and CREBBP. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with Yersinia yopJ.
      SUBCELLULAR LOCATION: Cytoplasm.
      TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
      PTM: Upon cytokine stimulation, phosphorylated on Ser-177 and Ser- 181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. & Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.
      SIMILARITY: SwissProt: O14920 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight87 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by western blot on human Jurkat RIPA cell lysate, human A431, HeLa nuclear extract, mouse 3T3/A31 or rat PC-12 RIPA cell lysates.

      Western Blot Analysis:
      0.5-2 µg/mL of this lot detected IKK/IKK2 in human HeLa cell lysate. A previous lot was tested in human Jurkat RIPA cell lysate, A431, extract, mouse 3T3/A31 and rat PC-12 RIPA cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IKK#946;, clone 10AG2 - DAM1749919 DAM1749919
      Anti-IKK#946;, clone 10AG2 - 2137999 2137999
      Anti-IKK, clone 10AG2 - 2064509 2064509
      Anti-IKK, clone 10AG2 - 2203116 2203116
      Anti-IKK, clone 10AG2 - 2290926 2290926
      Anti-IKK, clone 10AG2 - DAM1442517 DAM1442517
      Anti-IKK, clone 10AG2 - DAM1734752 DAM1734752
      Anti-IKK, clone 10AG2 - DAM1774677 DAM1774677
      Anti-IKK, clone 10AG2 - JBC1872234 JBC1872234
      Anti-IKKBeta, clone 10AG2 - DAM1543628 DAM1543628

      References

      Reference overviewApplicationSpeciesPub Med ID
      IKK is a therapeutic target in KRAS-Induced lung cancer with disrupted p53 activity.
      Bassères, DS; Ebbs, A; Cogswell, PC; Baldwin, AS
      Genes & cancer  5  41-55  2014

      Show Abstract
      Western Blotting24955217 24955217
      Intestinal tumorigenesis initiated by dedifferentiation and acquisition of stem-cell-like properties.
      Schwitalla, S; Fingerle, AA; Cammareri, P; Nebelsiek, T; Göktuna, SI; Ziegler, PK; Canli, O; Heijmans, J; Huels, DJ; Moreaux, G; Rupec, RA; Gerhard, M; Schmid, R; Barker, N; Clevers, H; Lang, R; Neumann, J; Kirchner, T; Taketo, MM; van den Brink, GR; Sansom, OJ; Arkan, MC; Greten, FR
      Cell  152  25-38  2013

      Show Abstract
      23273993 23273993
      Transcription of Tnfaip3 is regulated by NF-κB and p38 via C/EBPβ in activated macrophages.
      Lai, TY; Wu, SD; Tsai, MH; Chuang, EY; Chuang, LL; Hsu, LC; Lai, LC
      PloS one  8  e73153  2013

      Show Abstract
      Western BlottingMouse24023826 24023826
      IκB kinase β is required for activation of NF-κB and AP-1 in CD3/CD28-stimulated primary CD4(+) T cells.
      Lupino, E; Ramondetti, C; Piccinini, M
      Journal of immunology (Baltimore, Md. : 1950)  188  2545-55  2012

      Show Abstract
      22331067 22331067
      zVAD-induced necroptosis in L929 cells depends on autocrine production of TNFα mediated by the PKC-MAPKs-AP-1 pathway.
      Wu, YT; Tan, HL; Huang, Q; Sun, XJ; Zhu, X; Shen, HM
      Cell death and differentiation  18  26-37  2011

      Show Abstract
      20539307 20539307
      Crystal structure of inhibitor of κB kinase β.
      Xu, G; Lo, YC; Li, Q; Napolitano, G; Wu, X; Jiang, X; Dreano, M; Karin, M; Wu, H
      Nature  472  325-30  2011

      Show Abstract
      Western Blotting21423167 21423167
      NF-κB essential modulator (NEMO) interaction with linear and lys-63 ubiquitin chains contributes to NF-κB activation.
      Hadian, K; Griesbach, RA; Dornauer, S; Wanger, TM; Nagel, D; Metlitzky, M; Beisker, W; Schmidt-Supprian, M; Krappmann, D
      The Journal of biological chemistry  286  26107-17  2011

      Show Abstract
      21622571 21622571
      Requirement of the NF-kappaB subunit p65/RelA for K-Ras-induced lung tumorigenesis.
      Bassères, DS; Ebbs, A; Levantini, E; Baldwin, AS
      Cancer research  70  3537-46  2010

      Show Abstract
      20406971 20406971
      LPS induces KH-type splicing regulatory protein-dependent processing of microRNA-155 precursors in macrophages.
      Tina Ruggiero, Michele Trabucchi, Francesca De Santa, Simona Zupo, Brian D Harfe, Michael T McManus, M Geoff Rosenfeld, Paola Briata, Roberto Gherzi
      The FASEB journal : official publication of the Federation of American Societies for Experimental Biology  23  2898-908  2009

      Show Abstract
      Immunoblotting (Western)Mouse19423639 19423639
      Inactivation of NF-kappaB p50 leads to insulin sensitization in liver through post-translational inhibition of p70S6K.
      Zhanguo Gao,Jun Yin,Jin Zhang,Qing He,Owen P McGuinness,Jianping Ye
      The Journal of biological chemistry  284  2009

      Show Abstract Full Text Article
      19433583 19433583

      Brochure

      Title
      Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies