05-536 | Anti-IKKα Antibody, clone 14A231

05-536
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, MkWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-536
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IKKα Antibody, clone 14A231
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: Jurkat cell lysate (12-303)
      PresentationProtein G Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationAnti-IKKα Antibody, clone 14A231 is an antibody against IKKα for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenHis-tagged, full length human IKKα
      Clone14A231
      HostMouse
      SpecificityRecognizes IKKα.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      • Monkey
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
      Gene Symbol
      • CHUK
      • NFKBIKA
      • IKKA
      • IKBKA
      • IkBKA
      • TCF16
      • IKK1
      • IKK-A
      • IKK-alpha
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O15111 # Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B- regulated promoters during inflammatory responses triggered by cytokines.
      SIZE: 745 amino acids; 84654 Da
      SUBUNIT: Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP (By similarity). Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. A weak interaction with TRAF2 cannot be excluded. Part of a complex composed of NCOA2, NCOA3, IKKB, IKBKG and CREBBP. Interacts with NALP2. May interact with MAVS/IPS1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Shuttles between the cytoplasm and the nucleus.
      TISSUE SPECIFICITY: Widely expressed.
      PTM: Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
      SIMILARITY: SwissProt: O15111 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight85 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from Jurkat cells
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IKK#945;, clone 14A231 (mouse monoclonal IgG1) - DAM1823359 DAM1823359
      Anti-IKKa, clone 14A231 (mouse monoclonal IgG1) - 1947738 1947738
      Anti-IKKa, clone 14A231 (mouse monoclonal IgG1) - 2088650 2088650
      Anti-IKKa, clone 14A231 - DAM1817626 DAM1817626
      Anti-IKKa, clone 14A231 - JBC1872185 JBC1872185
      Anti-IKKa, clone 14A231 - JBC1900499 JBC1900499
      Anti-IKKalpha;, clone 14A231 - 19060 19060
      Anti-IKKalpha;, clone 14A231 - 20587 20587
      Anti-IKKalpha;, clone 14A231 - 21301 21301
      Anti-IKKalpha;, clone 14A231 - 23545 23545

      References

      Reference overviewApplicationPub Med ID
      IKK is a therapeutic target in KRAS-Induced lung cancer with disrupted p53 activity.
      Bassères, DS; Ebbs, A; Cogswell, PC; Baldwin, AS
      Genes & cancer  5  41-55  2014

      Show Abstract
      Western Blotting24955217 24955217
      NF-κB inducing kinase, NIK mediates cigarette smoke/TNFα-induced histone acetylation and inflammation through differential activation of IKKs.
      Chung, S; Sundar, IK; Hwang, JW; Yull, FE; Blackwell, TS; Kinnula, VL; Bulger, M; Yao, H; Rahman, I
      PloS one  6  e23488  2011

      Show Abstract
      21887257 21887257
      NF-κB essential modulator (NEMO) interaction with linear and lys-63 ubiquitin chains contributes to NF-κB activation.
      Hadian, K; Griesbach, RA; Dornauer, S; Wanger, TM; Nagel, D; Metlitzky, M; Beisker, W; Schmidt-Supprian, M; Krappmann, D
      The Journal of biological chemistry  286  26107-17  2011

      Show Abstract
      21622571 21622571
      RelB is differentially regulated by IkappaB Kinase-alpha in B cells and mouse lung by cigarette smoke.
      Yang, SR; Yao, H; Rajendrasozhan, S; Chung, S; Edirisinghe, I; Valvo, S; Fromm, G; McCabe, MJ; Sime, PJ; Phipps, RP; Li, JD; Bulger, M; Rahman, I
      American journal of respiratory cell and molecular biology  40  147-58  2009

      Show Abstract
      18688039 18688039
      IKK alpha causes chromatin modification on pro-inflammatory genes by cigarette smoke in mouse lung.
      Yang, SR; Valvo, S; Yao, H; Kode, A; Rajendrasozhan, S; Edirisinghe, I; Caito, S; Adenuga, D; Henry, R; Fromm, G; Maggirwar, S; Li, JD; Bulger, M; Rahman, I
      American journal of respiratory cell and molecular biology  38  689-98  2008

      Show Abstract
      18239189 18239189
      Essential role for epidermal growth factor receptor in glutamate receptor signaling to NF-kappaB.
      Sitcheran, R; Comb, WC; Cogswell, PC; Baldwin, AS
      Molecular and cellular biology  28  5061-70  2008

      Show Abstract
      Western Blotting18541671 18541671
      IkappaB kinase: beginning, not the end.
      Verma, I M and Stevenson, J
      Proc. Natl. Acad. Sci. U.S.A., 94: 11758-60 (1997)  1997

      9342307 9342307

      Brochure

      Title
      Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling

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      Included Positive Control

      Catalogue Number Description  
      12-303 Jurkat Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies