05-504 | Anti-IQGAP1 Antibody, clone AF4

05-504
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-504
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IQGAP1 Antibody, clone AF4
      References
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%
      Applications
      ApplicationDetect IQGAP1 using this Anti-IQGAP1 Antibody, clone AF4 validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenA Glu-Glu tagged C-terminal fragment corresponding to amino acids 863-1657 of human IQGAP1, purified from baculovirus infected insect cells
      Cloneclone AF4
      HostMouse
      SpecificityIQGAP1, not IQGAP2
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.
      Gene Symbol
      • IQGAP1
      • HUMORFA01
      • SAR1
      • KIAA0051
      • p195
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P46940 # Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth.
      SIZE: 1657 amino acids; 189252 Da
      SUBUNIT: Interacts with CDC42, both in its GTP-bound state and in its nucleotide-free state. Also interacts with RAC1, but not with RHOA.
      SUBCELLULAR LOCATION: Cell membrane.
      TISSUE SPECIFICITY: Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.
      DOMAIN: SwissProt: P46940 Regions C1 and C2 can either interact with nucleotide-free CDC42, or interact together, depending on the phosphorylation state of Ser-1443. When Ser-1443 is not phosphorylated, C1 and C2 interact, which prevents binding of nucleotide-free CDC42 and promotes binding of GTP-bound CDC42. Phosphorylation of Ser-1443 prevents interaction between C1 and C2, which opens the structure of the C-terminus and allows binding and sequestration of nucleotide-free CDC42 on both C1 and C2.
      PTM: Phosphorylation of Ser-1443 by PKC prevents interaction between C1 and C2, allowing binding of nucleotide-free CDC42. Ser- 1443 phosphorylation enhances the ability to promote neurite outgrowth.
      SIMILARITY: Contains 1 CH (calponin-homology) domain. & Contains 4 IQ domains. & Contains 1 Ras-GAP domain. & Contains 1 WW domain.
      Molecular Weight185kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HepG2 RIPA cell lysate
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IQGAP1, clone A/F4 (mouse monoclonal IgG) 2897070
      Anti-IQGAP1, clone AF4 (mouse monoclonal IgG) 2943752
      Anti-IQGAP1, clone AF4 (mouse monoclonal IgG) - 2032851 2032851
      Anti-IQGAP1, clone AF4 (mouse monoclonal IgG) - 2275614 2275614
      Anti-IQGAP1, clone AF4 (mouse monoclonal IgG) - JBC1926728 JBC1926728
      Anti-IQGAP1, clone AF4 - 18884 18884
      Anti-IQGAP1, clone AF4 - 2495651 2495651
      Anti-IQGAP1, clone AF4 - 26167 26167
      Anti-IQGAP1, clone AF4 - 32541 32541
      Anti-IQGAP1, clone AF4 - DAM1493337 DAM1493337

      References

      Reference overviewPub Med ID
      IQGAP1 involvement in MTOC and granule polarization in NK-cell cytotoxicity.
      Namita Kanwar,John A Wilkins
      European journal of immunology  41  2011

      Show Abstract
      21681737 21681737
      The zinc- and calcium-binding S100B interacts and co-localizes with IQGAP1 during dynamic rearrangement of cell membranes.
      Mbele, GO; Deloulme, JC; Gentil, BJ; Delphin, C; Ferro, M; Garin, J; Takahashi, M; Baudier, J
      The Journal of biological chemistry  277  49998-50007  2002

      Show Abstract
      12377780 12377780
      Cdc42, Rac1, and their effector IQGAP1 as molecular switches for cadherin-mediated cell-cell adhesion.
      Kuroda, S, et al.
      Biochem. Biophys. Res. Commun., 262: 1-6 (1999)  1999

      Show Abstract
      10448058 10448058
      Binding of myosin essential light chain to the cytoskeleton-associated protein IQGAP1.
      Weissbach, L, et al.
      Biochem. Biophys. Res. Commun., 251: 269-76 (1998)  1998

      Show Abstract
      9790945 9790945
      IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a potential effector for cdc42Hs.
      Hart, M J, et al.
      EMBO J., 15: 2997-3005 (1996)  1996

      Show Abstract
      8670801 8670801

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies