06-872 | Anti-IRAK Antibody

06-872
200 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MIP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number06-872
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IRAK Antibody
      References
      Product Information
      FormatPurified
      Control
      • Positive Control Included: HeLa Nuclear Extract (12-309).
      PresentationProtein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
      Applications
      ApplicationDetect IRAK using this Anti-IRAK Antibody validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenPeptide (DRQGPEESDEFQS) corresponding to amino acids 700-712 of human IRAK
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes IRAK.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • IRAK1
      • IRAK
      • pelle
      • IRAK-1
      Purification MethodAmmonium sulfate precipitation and DEAE-cellulose chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P51617 # Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1.
      COFACTOR: Magnesium.
      SIZE: 712 amino acids; 76537 Da
      SUBUNIT: IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Interacts with IL1RL1.
      TISSUE SPECIFICITY: Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.
      PTM: Autophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
      SIMILARITY: SwissProt: P51617 ## Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily. & Contains 1 protein kinase domain.
      Molecular Weight80 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on HeLa nuclear extract
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IRAK (rabbit polyclonal IgG) - 2362876 2362876
      Anti-IRAK (rabbit polyclonal IgG) - 2270296 2270296
      Anti-IRAK (rabbit polyclonal IgG) -2504283 2504283
      Anti-IRAK - 17526 17526
      Anti-IRAK - 19703 19703
      Anti-IRAK - 21500 21500
      Anti-IRAK - 23104 23104
      Anti-IRAK - 24707 24707
      Anti-IRAK - 2535271 2535271
      Anti-IRAK - 26526 26526

      References

      Reference overviewApplicationPub Med ID
      Genetically modified "obligate" anaerobic Salmonella typhimurium as a therapeutic strategy for neuroblastoma.
      Guo, ZL; Yu, B; Ning, BT; Chan, S; Lin, QB; Li, JC; Huang, JD; Chan, GC
      Journal of hematology & oncology  8  99  2015

      Show Abstract
      26286454 26286454
      Rotavirus Non-Structural Protein-1 suppresses virus induced cellular apoptosis to facilitate viral growth by activating the cell-survival pathways during early stages of infection.
      Bagchi P, Dutta D, Chattopadhyay S, Mukherjee A, Halder UC, Sarkar S, Kobayashi N, Komoto S, Taniguchi K, Chawla-Sarkar M
      J Virol  2010

      Show Abstract
      20392855 20392855
      Cigarette smoke induces CXCL8 production by human neutrophils via activation of TLR9 receptor.
      Mortaz, E; Adcock, IM; Ito, K; Kraneveld, AD; Nijkamp, FP; Folkerts, G
      The European respiratory journal  36  1143-54  2010

      Show Abstract
      19840968 19840968
      Selective control of type I IFN induction by the Rac activator DOCK2 during TLR-mediated plasmacytoid dendritic cell activation.
      Gotoh, K; Tanaka, Y; Nishikimi, A; Nakamura, R; Yamada, H; Maeda, N; Ishikawa, T; Hoshino, K; Uruno, T; Cao, Q; Higashi, S; Kawaguchi, Y; Enjoji, M; Takayanagi, R; Kaisho, T; Yoshikai, Y; Fukui, Y
      The Journal of experimental medicine  207  721-30  2010

      Show Abstract
      Western Blotting20231379 20231379
      Candida albicans triggers activation of distinct signaling pathways to establish a proinflammatory gene expression program in primary human endothelial cells.
      Verena Müller, Dorothee Viemann, Marc Schmidt, Nicole Endres, Stephan Ludwig, Martin Leverkus, Johannes Roth, Matthias Goebeler
      Journal of immunology (Baltimore, Md. : 1950)  179  8435-45  2007

      Show Abstract
      18056390 18056390
      Neutrophil signaling pathways activated by bacterial DNA stimulation.
      María E Alvarez, Juan I Fuxman Bass, Jorge R Geffner, Paula X Fernández Calotti, Mónica Costas, Omar A Coso, Romina Gamberale, Mónica E Vermeulen, Gabriela Salamone, Diego Martinez, Tamara Tanos, Analía S Trevani
      Journal of immunology (Baltimore, Md. : 1950)  177  4037-46  2006

      Show Abstract
      16951367 16951367
      Regulation of IL-1 receptor-associated kinases by lipopolysaccharide
      Hu, J., et al
      J Immunol, 168:3910-4 (2002)  2002

      Immunoblotting (Western)11937546 11937546
      Characterization of interleukin-1 receptor-associated kinase in normal and endotoxin-tolerant cells
      Li, L., et al
      J Biol Chem, 275:23340-5 (2000)  2000

      Immunoprecipitation10811644 10811644
      IRAK: a kinase associated with the interleukin-1 receptor.
      Cao, Z, et al.
      Science, 271: 1128-31 (1996)  1996

      Show Abstract
      8599092 8599092
      Developmental and tissue-specific expression of mouse pelle-like protein kinase.
      Trofimova, M, et al.
      J. Biol. Chem., 271: 17609-12 (1996)  1996

      Show Abstract
      8663605 8663605

      Brochure

      Title
      Pathways and Biomarkers of Toll-like Receptor (TLR) Signaling

      Related Products & Applications

      Included Positive Control

      Catalogue Number Description  
      12-309 HeLa Nuclear Extract Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies