06-248 | Anti-IRS1 Antibody

06-248
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number06-248
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-IRS1 Antibody
      Background InformationInsulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Included Positive Antigen Control: Catalog # 12-305, 3T3/A31 Cell Lysate. Use 20 µg per lane for minigels.
      PresentationPurified Rabbit IgG in buffer containing 0.2 M Trisglycine, pH 7.4, 0.15 M NaCl, 0.1 mM EDTA, 5 mg/mL BSA, and 0.05% sodium azide.
      Applications
      ApplicationAnti-IRS1 Antibody is an antibody against IRS1 for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Biological Information
      ImmunogenCarboxy-terminal 14 amino acid peptide ([C]YASINFQKQPEDRQ) of rat liver IRS1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes IRS1, Mr 160-185kDa..
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IRS1
      • IRS-1
      • HIRS-1
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).
      SIZE: 1242 amino acids; 131591 Da
      SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.
      PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].
      SIMILARITY: SwissProt: P35568 ## Contains 1 IRS-type PTB domain. & Contains 1 PH domain.
      Molecular Weight165 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on NIH3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Anti-IRS1 2465193
      Anti-IRS1 - 2387826 2387826
      Anti-IRS1 - 2430423 2430423
      Anti-IRS1 - DAM1791335 DAM1791335
      Anti-IRS1 - 16503 16503
      Anti-IRS1 - 16945 16945
      Anti-IRS1 - 17612 17612
      Anti-IRS1 - 17966 17966
      Anti-IRS1 - 18621 18621
      Anti-IRS1 - 19407 19407

      References

      Reference overviewApplicationSpeciesPub Med ID
      Endoplasmic reticulum stress impairs insulin receptor signaling in the brains of obese rats.
      Liang, L; Chen, J; Zhan, L; Lu, X; Sun, X; Sui, H; Zheng, L; Xiang, H; Zhang, F
      PloS one  10  e0126384  2015

      Show Abstract
      25978724 25978724
      Role of protein farnesylation in burn-induced metabolic derangements and insulin resistance in mouse skeletal muscle.
      Nakazawa, H; Yamada, M; Tanaka, T; Kramer, J; Yu, YM; Fischman, AJ; Martyn, JA; Tompkins, RG; Kaneki, M
      PloS one  10  e0116633  2015

      Show Abstract
      Western Blotting25594415 25594415
      Class III PI3K regulates organismal glucose homeostasis by providing negative feedback on hepatic insulin signalling.
      Nemazanyy, I; Montagnac, G; Russell, RC; Morzyglod, L; Burnol, AF; Guan, KL; Pende, M; Panasyuk, G
      Nature communications  6  8283  2015

      Show Abstract
      26387534 26387534
      Diet-induced obesity in mice reduces placental efficiency and inhibits placental mTOR signaling.
      Lager, S; Samulesson, AM; Taylor, PD; Poston, L; Powell, TL; Jansson, T
      Physiological reports  2  e00242  2014

      Show Abstract
      Western Blotting24744907 24744907
      Oxidative stress and altered lipid homeostasis in the programming of offspring fatty liver by maternal obesity.
      Alfaradhi, MZ; Fernandez-Twinn, DS; Martin-Gronert, MS; Musial, B; Fowden, A; Ozanne, SE
      American journal of physiology. Regulatory, integrative and comparative physiology  307  R26-34  2014

      Show Abstract
      Western BlottingMouse24789994 24789994
      PTEN is a protein tyrosine phosphatase for IRS1.
      Shi, Y; Wang, J; Chandarlapaty, S; Cross, J; Thompson, C; Rosen, N; Jiang, X
      Nature structural & molecular biology  21  522-7  2014

      Show Abstract
      24814346 24814346
      In vivo siRNA delivery of Keap1 modulates death and survival signaling pathways and attenuates concanavalin-A-induced acute liver injury in mice.
      González-Rodríguez, Á; Reibert, B; Amann, T; Constien, R; Rondinone, CM; Valverde, ÁM
      Disease models & mechanisms  7  1093-100  2014

      Show Abstract
      24997191 24997191
      Essential role of protein-tyrosine phosphatase 1B in the modulation of insulin signaling by acetaminophen in hepatocytes.
      Mobasher, MA; de Toro-Martín, J; González-Rodríguez, Á; Ramos, S; Letzig, LG; James, LP; Muntané, J; Álvarez, C; Valverde, ÁM
      The Journal of biological chemistry  289  29406-19  2014

      Show Abstract
      25204659 25204659
      Inhibition of Cullin-RING E3 ubiquitin ligase 7 by simian virus 40 large T antigen.
      Hartmann, T; Xu, X; Kronast, M; Muehlich, S; Meyer, K; Zimmermann, W; Hurwitz, J; Pan, ZQ; Engelhardt, S; Sarikas, A
      Proceedings of the National Academy of Sciences of the United States of America  111  3371-6  2014

      Show Abstract
      Immunofluorescence24550499 24550499
      Variant NKX3.1 and Serum IGF-1: Investigation of Interaction in Prostate Cancer.
      Muhlbradt, E; Ma, J; Severi, G; Ortner, E; Hayes, V; Hoang, HN; Stampfer, M; Giles, G; Pollak, M; Gelmann, EP
      Genes & cancer  4  535-45  2013

      Show Abstract
      24386513 24386513

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      Included Positive Control

      Catalogue Number Description  
      12-305 3T3/A31 Cell Lysate Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies