CBL479 | Anti-Integrin β3 Antibody, clone PM6/13

CBL479
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IP, WB, IHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberCBL479
      ReplacesMAB1381
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β3 Antibody, clone PM6/13
      Alternate Names
      • CD61
      References
      Product Information
      FormatPurified
      PresentationThe monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Applications
      ApplicationThis Anti-Integrin β3 Antibody, clone PM6/13 is validated for use in FC, IP, WB, IH, IH(P) for the detection of Integrin β3.
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesFACS: As a marker for megakaryoblastic/cytic leukaemias and idiopathic thrombocythemia





      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenHuman platelet plasma membrane
      ClonePM6/13
      HostMouse
      SpecificityThe antibody is specific for the 110 kDa platelet glycoprotein GP IIIa and inhibits platelet aggregation and activation induced by thrombin and collagen. The platelet glycoprotein is the integrin beta3 chain of the vitronectin receptor and GPIIb/IIIa. The antigen is present on platelets and megakaryocytes.

      Antigen distribution:

      Platelets > 98%

      Megakaryocytes > 98%

      Granulocytes 0%

      Monocytes 0%

      LGL cells 0%

      B cells 0%

      T cells 0%

      Peripheral blood lymphocytes 0%

      FUSION PARTNER: SP2/0 myeloma cell line
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
      Gene Symbol
      • ITGB3
      • GP3A
      • CD61
      • GPIIIa
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05106 # Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 788 amino acids; 87058 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
      PTM: Phosphorylated on tyrosine residues in response to thrombin- induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. SHC binding also occurs when Tyr-785 alone is phosphorylated.
      DISEASE: SwissProt: P05106 # Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.
      SIMILARITY: SwissProt: P05106 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      SDS

      Title

      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-HUMAN CD61 2487697
      MOUSE ANTI-HUMAN CD61 MONOCLONAL ANTIBODY - 2427483 2427483
      MOUSE ANTI-HUMAN CD61 - 2154070 2154070
      MOUSE ANTI-HUMAN CD61 MONOCLONAL ANTIBODY - 2106896 2106896
      MOUSE ANTI-HUMAN CD61 MONOCLONAL ANTIBODY - 2327720 2327720

      References

      Reference overviewSpeciesPub Med ID
      A novel family of RGD-containing disintegrins (Tablysin-15) from the salivary gland of the horsefly Tabanus yao targets αIIbβ3 or αVβ3 and inhibits platelet aggregation and angiogenesis.
      Ma, D; Xu, X; An, S; Liu, H; Yang, X; Andersen, JF; Wang, Y; Tokumasu, F; Ribeiro, JM; Francischetti, IM; Lai, R
      Thrombosis and haemostasis  105  1032-45  2011

      Show Abstract
      21475772 21475772
      Role of the beta-subunit arginine/lysine finger in integrin heterodimer formation and function.
      Vineet Gupta,José Luis Alonso,Takashi Sugimori,Makram Essafi,Makram Issafi,Jiang-Ping Xiong,M Amin Arnaout
      Journal of immunology (Baltimore, Md. : 1950)  180  2008

      Show Abstract Full Text Article
      18209068 18209068
      Pharmacology of the novel antiangiogenic peptide ATN-161 (Ac-PHSCN-NH2): observation of a U-shaped dose-response curve in several preclinical models of angiogenesis and tumor growth.
      Doñate, F; Parry, GC; Shaked, Y; Hensley, H; Guan, X; Beck, I; Tel-Tsur, Z; Plunkett, ML; Manuia, M; Shaw, DE; Kerbel, RS; Mazar, AP
      Clinical cancer research : an official journal of the American Association for Cancer Research  14  2137-44  2008

      Show Abstract
      18381955 18381955
      FAK-independent alphavbeta3 integrin-EGFR complexes rescue from anoikis matrix-defective fibroblasts.
      Zoppi, Nicoletta, et al.
      Biochim. Biophys. Acta, 1783: 1177-88 (2008)  2008

      Human18405669 18405669
      Loss of dipeptidyl peptidase IV immunostaining discriminates malignant melanomas from deep penetrating nevi.
      Roesch, A; Wittschier, S; Becker, B; Landthaler, M; Vogt, T
      Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc  19  1378-85  2006

      Show Abstract
      16829852 16829852
      On the mechanism of thrombin-induced angiogenesis: involvement of alphavbeta3-integrin.
      Tsopanoglou, Nikos E, et al.
      Am. J. Physiol., Cell Physiol., 283: C1501-10 (2002)  2002

      Show Abstract
      12372811 12372811

      Data Sheet

      Title
      Anti-Integrin beta3, clone PM6/13 - Data Sheet

      Related Products & Applications

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      Alternative Packsize

      Catalogue Number Description  
      CBL479-K Anti-Integrin Β3, Clone Pm6/13 (1mg) Show Pricing & Availability

      Alternative Format

      Catalogue Number Description  
      CBL479F Anti-Integrin β3 Antibody, FITC conjugated, clone PM 6/13 Show Pricing & Availability
      CBL479P Anti-Integrin β3 Antibody, clone PM 6/13, Phycoerythrin conjugated Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies